BIOMARKERS

Molecular Biopsy of Human Tumors

- a resource for Precision Medicine *

360 related articles for article (PubMed ID: 11545587)

  • 1. Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies.
    Bach H; Mazor Y; Shaky S; Shoham-Lev A; Berdichevsky Y; Gutnick DL; Benhar I
    J Mol Biol; 2001 Sep; 312(1):79-93. PubMed ID: 11545587
    [TBL] [Abstract][Full Text] [Related]  

  • 2. Thioredoxin fusions increase folding of single chain Fv antibodies in the cytoplasm of Escherichia coli: evidence that chaperone activity is the prime effect of thioredoxin.
    Jurado P; de Lorenzo V; Fernández LA
    J Mol Biol; 2006 Mar; 357(1):49-61. PubMed ID: 16427080
    [TBL] [Abstract][Full Text] [Related]  

  • 3. A universal strategy for stable intracellular antibodies.
    Shaki-Loewenstein S; Zfania R; Hyland S; Wels WS; Benhar I
    J Immunol Methods; 2005 Aug; 303(1-2):19-39. PubMed ID: 16045924
    [TBL] [Abstract][Full Text] [Related]  

  • 4. Improved expression characteristics of single-chain Fv fragments when fused downstream of the Escherichia coli maltose-binding protein or upstream of a single immunoglobulin-constant domain.
    Hayhurst A
    Protein Expr Purif; 2000 Feb; 18(1):1-10. PubMed ID: 10648163
    [TBL] [Abstract][Full Text] [Related]  

  • 5. Production of a soluble and active MBP-scFv fusion: favorable effect of the leaky tolR strain.
    Chames P; Fieschi J; Baty D
    FEBS Lett; 1997 Mar; 405(2):224-8. PubMed ID: 9089295
    [TBL] [Abstract][Full Text] [Related]  

  • 6. Production of stabilized scFv antibody fragments in the E. coli bacterial cytoplasm.
    Vaks L; Benhar I
    Methods Mol Biol; 2014; 1060():171-84. PubMed ID: 24037842
    [TBL] [Abstract][Full Text] [Related]  

  • 7. Order of fusions between bacterial and mammalian proteins can determine solubility in Escherichia coli.
    Sachdev D; Chirgwin JM
    Biochem Biophys Res Commun; 1998 Mar; 244(3):933-7. PubMed ID: 9535771
    [TBL] [Abstract][Full Text] [Related]  

  • 8. Production of Stabilized Antibody Fragments in the E. coli Bacterial Cytoplasm and in Transiently Transfected Mammalian Cells.
    Birnboim-Perach R; Grinberg Y; Vaks L; Nahary L; Benhar I
    Methods Mol Biol; 2019; 1904():455-480. PubMed ID: 30539486
    [TBL] [Abstract][Full Text] [Related]  

  • 9. Single amino acid substitutions on the surface of Escherichia coli maltose-binding protein can have a profound impact on the solubility of fusion proteins.
    Fox JD; Kapust RB; Waugh DS
    Protein Sci; 2001 Mar; 10(3):622-30. PubMed ID: 11344330
    [TBL] [Abstract][Full Text] [Related]  

  • 10. Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin.
    Sachdev D; Chirgwin JM
    Protein Expr Purif; 1998 Feb; 12(1):122-32. PubMed ID: 9473466
    [TBL] [Abstract][Full Text] [Related]  

  • 11. Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli.
    Jurado P; Ritz D; Beckwith J; de Lorenzo V; Fernández LA
    J Mol Biol; 2002 Jun; 320(1):1-10. PubMed ID: 12079330
    [TBL] [Abstract][Full Text] [Related]  

  • 12. Functional expression of single-chain Fv antibody in the cytoplasm of Escherichia coli by thioredoxin fusion and co-expression of molecular chaperones.
    Sonoda H; Kumada Y; Katsuda T; Yamaji H
    Protein Expr Purif; 2010 Apr; 70(2):248-53. PubMed ID: 19913620
    [TBL] [Abstract][Full Text] [Related]  

  • 13. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused.
    Kapust RB; Waugh DS
    Protein Sci; 1999 Aug; 8(8):1668-74. PubMed ID: 10452611
    [TBL] [Abstract][Full Text] [Related]  

  • 14. Activity of protein MalE (maltose-binding protein) fused to cytoplasmic and periplasmic regions of an Escherichia coli inner membrane protein.
    Dassa E; Lambert P
    Res Microbiol; 1997 Jun; 148(5):389-95. PubMed ID: 9765817
    [TBL] [Abstract][Full Text] [Related]  

  • 15. Functional dissection of a cell-division inhibitor, SulA, of Escherichia coli and its negative regulation by Lon.
    Higashitani A; Ishii Y; Kato Y; Koriuchi K
    Mol Gen Genet; 1997 Apr; 254(4):351-7. PubMed ID: 9180687
    [TBL] [Abstract][Full Text] [Related]  

  • 16. Functional expression of single-chain variable fragment antibody against c-Met in the cytoplasm of Escherichia coli.
    Heo MA; Kim SH; Kim SY; Kim YJ; Chung J; Oh MK; Lee SG
    Protein Expr Purif; 2006 May; 47(1):203-9. PubMed ID: 16414274
    [TBL] [Abstract][Full Text] [Related]  

  • 17. Vectors to facilitate the creation of translational fusions to the maltose-binding protein of Escherichia coli.
    Aitken R; Gilchrist J; Sinclair MC
    Gene; 1994 Jun; 144(1):69-73. PubMed ID: 8026760
    [TBL] [Abstract][Full Text] [Related]  

  • 18. Overexpression of bacterio-opsin in Escherichia coli as a water-soluble fusion to maltose binding protein: efficient regeneration of the fusion protein and selective cleavage with trypsin.
    Chen GQ; Gouaux JE
    Protein Sci; 1996 Mar; 5(3):456-67. PubMed ID: 8868482
    [TBL] [Abstract][Full Text] [Related]  

  • 19. Engineering stable cytoplasmic intrabodies with designed specificity.
    Donini M; Morea V; Desiderio A; Pashkoulov D; Villani ME; Tramontano A; Benvenuto E
    J Mol Biol; 2003 Jul; 330(2):323-32. PubMed ID: 12823971
    [TBL] [Abstract][Full Text] [Related]  

  • 20. Characterization of two human flavin-containing monooxygenase (form 3) enzymes expressed in Escherichia coli as maltose binding protein fusions.
    Brunelle A; Bi YA; Lin J; Russell B; Luy L; Berkman C; Cashman J
    Drug Metab Dispos; 1997 Aug; 25(8):1001-7. PubMed ID: 9280409
    [TBL] [Abstract][Full Text] [Related]  

    [Next]    [New Search]
    of 18.