130 related articles for article (PubMed ID: 12586400)
1. Deacetoxycephalosporin C synthase isozymes exhibit diverse catalytic activity and substrate specificity.
Chin HS; Sim J; Seah KI; Sim TS
FEMS Microbiol Lett; 2003 Jan; 218(2):251-7. PubMed ID: 12586400
[TBL] [Abstract][Full Text] [Related]
2. Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase.
Lloyd MD; Lipscomb SJ; Hewitson KS; Hensgens CM; Baldwin JE; Schofield CJ
J Biol Chem; 2004 Apr; 279(15):15420-6. PubMed ID: 14734549
[TBL] [Abstract][Full Text] [Related]
3. Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase.
Kovacevic S; Weigel BJ; Tobin MB; Ingolia TD; Miller JR
J Bacteriol; 1989 Feb; 171(2):754-60. PubMed ID: 2644235
[TBL] [Abstract][Full Text] [Related]
4. C-terminus modification of Streptomyces clavuligerus deacetoxycephalosporin C synthase improves catalysis with an expanded substrate specificity.
Chin HS; Sim TS
Biochem Biophys Res Commun; 2002 Jul; 295(1):55-61. PubMed ID: 12083766
[TBL] [Abstract][Full Text] [Related]
5. Conformational flexibility of the C terminus with implications for substrate binding and catalysis revealed in a new crystal form of deacetoxycephalosporin C synthase.
Oster LM; van Scheltinga AC; Valegård K; Hose AM; Dubus A; Hajdu J; Andersson I
J Mol Biol; 2004 Oct; 343(1):157-71. PubMed ID: 15381427
[TBL] [Abstract][Full Text] [Related]
6. Mutation of N304 to leucine in Streptomyces clavuligerus deacetoxycephalosporin C synthase creates an enzyme with increased penicillin analogue conversion.
Chin HS; Sim J; Sim TS
Biochem Biophys Res Commun; 2001 Sep; 287(2):507-13. PubMed ID: 11554757
[TBL] [Abstract][Full Text] [Related]
7. A complete library of amino acid alterations at R306 in Streptomyces clavuligerus deacetoxycephalosporin C synthase demonstrates its structural role in the ring-expansion activity.
Sim Goo K; Song Chua C; Sim TS
Proteins; 2008 Feb; 70(3):739-47. PubMed ID: 17729280
[TBL] [Abstract][Full Text] [Related]
8. Contrasting fates for 6-alpha-methylpenicillin N upon oxidation by deacetoxycephalosporin C synthase (DAOCS) and deacetoxy/deacetylcephalosporin C synthase (DAOC/DACS).
Hamilton CS; Yasuhara A; Baldwin JE; Lloyd MD; Rutledge PJ
Bioorg Med Chem Lett; 2001 Sep; 11(18):2511-4. PubMed ID: 11549458
[TBL] [Abstract][Full Text] [Related]
9. Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS).
Lee HJ; Lloyd MD; Harlos K; Clifton IJ; Baldwin JE; Schofield CJ
J Mol Biol; 2001 May; 308(5):937-48. PubMed ID: 11352583
[TBL] [Abstract][Full Text] [Related]
10. Mutational evidence supporting the involvement of tripartite residues His183, Asp185, and His243 in Streptomyces clavuligerus deacetoxycephalosporin C synthase for catalysis.
Sim J; Sim TS
Biosci Biotechnol Biochem; 2000 Apr; 64(4):828-32. PubMed ID: 10830499
[TBL] [Abstract][Full Text] [Related]
11. Probing the penicillin sidechain selectivity of recombinant deacetoxycephalosporin C synthase.
Dubus A; Lloyd MD; Lee HJ; Schofield CJ; Baldwin JE; Frere JM
Cell Mol Life Sci; 2001 May; 58(5-6):835-43. PubMed ID: 11437242
[TBL] [Abstract][Full Text] [Related]
12. The role of arginine residues in substrate binding and catalysis by deacetoxycephalosporin C synthase.
Lipscomb SJ; Lee HJ; Mukherji M; Baldwin JE; Schofield CJ; Lloyd MD
Eur J Biochem; 2002 Jun; 269(11):2735-9. PubMed ID: 12047382
[TBL] [Abstract][Full Text] [Related]
13. A complete library of amino acid alterations at N304 in Streptomyces clavuligerus deacetoxycephalosporin C synthase elucidates the basis for enhanced penicillin analogue conversion.
Chin HS; Goo KS; Sim TS
Appl Environ Microbiol; 2004 Jan; 70(1):607-9. PubMed ID: 14711695
[TBL] [Abstract][Full Text] [Related]
14. Relevant double mutations in bioengineered Streptomyces clavuligerus deacetoxycephalosporin C synthase result in higher binding specificities which improve penicillin bioconversion.
Goo KS; Chua CS; Sim TS
Appl Environ Microbiol; 2008 Feb; 74(4):1167-75. PubMed ID: 18083859
[TBL] [Abstract][Full Text] [Related]
15. Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin G.
Gao Q; Piret JM; Adrio JL; Demain AL
J Ind Microbiol Biotechnol; 2003 Mar; 30(3):190-4. PubMed ID: 12715257
[TBL] [Abstract][Full Text] [Related]
16. Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus.
Dotzlaf JE; Yeh WK
J Biol Chem; 1989 Jun; 264(17):10219-27. PubMed ID: 2656705
[TBL] [Abstract][Full Text] [Related]
17. The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S. clavuligerus.
Lee HJ; Lloyd MD; Harlos K; Schofield CJ
Biochem Biophys Res Commun; 2000 Jan; 267(1):445-8. PubMed ID: 10623639
[TBL] [Abstract][Full Text] [Related]
18. Family shuffling of expandase genes to enhance substrate specificity for penicillin G.
Hsu JS; Yang YB; Deng CH; Wei CL; Liaw SH; Tsai YC
Appl Environ Microbiol; 2004 Oct; 70(10):6257-63. PubMed ID: 15466573
[TBL] [Abstract][Full Text] [Related]
19. Oxygen derepresses deacetoxycephalosporin C synthase and increases the conversion of penicillin N to cephamycin C in Streptomyces clavuligerus.
Rollins MJ; Jensen SE; Wolfe S; Westlake DW
Enzyme Microb Technol; 1990 Jan; 12(1):40-5. PubMed ID: 1366380
[TBL] [Abstract][Full Text] [Related]
20. Extracellular production of biologically active deacetoxycephalosporin C synthase from Streptomyces clavuligerus in Pichia pastoris.
Adrio JL; Velasco J; Soler G; Rodriguez-Saiz M; Barredo JL; Moreno MA
Biotechnol Bioeng; 2001 Nov; 75(4):485-91. PubMed ID: 11668449
[TBL] [Abstract][Full Text] [Related]
[Next] [New Search]
