121 related articles for article (PubMed ID: 12969565)
1. A general method allowing the design of oligonucleotide primers to amplify the variable regions from immunoglobulin cDNA.
Essono S; Frobert Y; Grassi J; Créminon C; Boquet D
J Immunol Methods; 2003 Aug; 279(1-2):251-66. PubMed ID: 12969565
[TBL] [Abstract][Full Text] [Related]
2. [A novel method of amplifying the heavy-chain variable regions from immunoglobulin cDNAs].
Wang Y; Chen W; Li X; Cheng B
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi; 2006 Dec; 23(6):1308-13, 1319. PubMed ID: 17228732
[TBL] [Abstract][Full Text] [Related]
3. Optimization of primers for cloning libraries of mouse immunoglobulin genes using the polymerase chain reaction.
Kettleborough CA; Saldanha J; Ansell KH; Bendig MM
Eur J Immunol; 1993 Jan; 23(1):206-11. PubMed ID: 8419173
[TBL] [Abstract][Full Text] [Related]
4. Molecular cloning and sequence analysis of heavy- and light-chain variable region genes of anti-CD71 monoclonal antibody.
Wang ZY; Shen T; Zhang AH; Bi L; Jin YL; Sun KF; Yu MS; Shi LR
Di Yi Jun Yi Da Xue Xue Bao; 2002 May; 22(5):409-11. PubMed ID: 12390698
[TBL] [Abstract][Full Text] [Related]
5. Universal PCR amplification of mouse immunoglobulin gene variable regions: the design of degenerate primers and an assessment of the effect of DNA polymerase 3' to 5' exonuclease activity.
Wang Z; Raifu M; Howard M; Smith L; Hansen D; Goldsby R; Ratner D
J Immunol Methods; 2000 Jan; 233(1-2):167-77. PubMed ID: 10648866
[TBL] [Abstract][Full Text] [Related]
6. Degenerate primer design to clone the human repertoire of immunoglobulin heavy chain variable regions.
Sun Y; Liu HY; Mu L; Luo EJ
World J Microbiol Biotechnol; 2012 Jan; 28(1):381-6. PubMed ID: 22806814
[TBL] [Abstract][Full Text] [Related]
7. Nested polymerase chain reaction amplification and sequencing analysis of the light-chain and heavy-chain variable regions in the influenza A H1N1 virus hemagglutinin monoclonal antibody gene.
Li HJ; Guo CY; Sun JY; Sun LJ; Zhao PH; Hu L; Li Y; Hu J
Genet Mol Res; 2014 Jun; 13(2):4372-9. PubMed ID: 25036343
[TBL] [Abstract][Full Text] [Related]
8. [Amplification, cloning and sequence analysis of the variable region genes of monoclonal antibody against human bladder carcinoma].
Xiao S; Yu L; Gu Z
Zhonghua Wai Ke Za Zhi; 1996 Jan; 34(1):13-5. PubMed ID: 9275679
[TBL] [Abstract][Full Text] [Related]
9. A simplified workflow for monoclonal antibody sequencing.
Meyer L; López T; Espinosa R; Arias CF; Vollmers C; DuBois RM
PLoS One; 2019; 14(6):e0218717. PubMed ID: 31233538
[TBL] [Abstract][Full Text] [Related]
10. Degenerated primer design to amplify the heavy chain variable region from immunoglobulin cDNA.
Wang Y; Chen W; Li X; Cheng B
BMC Bioinformatics; 2006 Dec; 7 Suppl 4(Suppl 4):S9. PubMed ID: 17217527
[TBL] [Abstract][Full Text] [Related]
11. Cloning of cDNAs encoding the variable domains of antibody KC4G3 and construction of a chimeric antibody.
Couto JR; Blank EW; Peterson JA; Ceriani RL
Hybridoma; 1993 Aug; 12(4):485-9. PubMed ID: 8244420
[TBL] [Abstract][Full Text] [Related]
12. Systematic design and testing of nested (RT-)PCR primers for specific amplification of mouse rearranged/expressed immunoglobulin variable region genes from small number of B cells.
Rohatgi S; Ganju P; Sehgal D
J Immunol Methods; 2008 Dec; 339(2):205-19. PubMed ID: 18926828
[TBL] [Abstract][Full Text] [Related]
13. Cloning immunoglobulin variable domains for expression by the polymerase chain reaction.
Orlandi R; Güssow DH; Jones PT; Winter G
Proc Natl Acad Sci U S A; 1989 May; 86(10):3833-7. PubMed ID: 2726754
[TBL] [Abstract][Full Text] [Related]
14. An extended primer set for PCR amplification of murine kappa variable regions.
Leung SO; Dion AS; Pellegrini MC; Goldenberg DM; Hansen HJ
Biotechniques; 1993 Aug; 15(2):286-92. PubMed ID: 8373597
[TBL] [Abstract][Full Text] [Related]
15. Bacterial single-chain antibody fragments, specific for carcinoembryonic antigen.
Ayala M; Dueñas M; Santos A; Vázquez J; Menéndez A; Silva A; Gavilondo JV
Biotechniques; 1992 Nov; 13(5):790-9. PubMed ID: 1418981
[TBL] [Abstract][Full Text] [Related]
16. Cloning of cDNAs encoding the variable domains of antibody BrE-3 and construction of a chimeric antibody.
Couto JR; Blank EW; Peterson JA; Ceriani RL
Hybridoma; 1993 Feb; 12(1):15-23. PubMed ID: 8454302
[TBL] [Abstract][Full Text] [Related]
17. Novel vectors for the expression of antibody molecules using variable regions generated by polymerase chain reaction.
Coloma MJ; Hastings A; Wims LA; Morrison SL
J Immunol Methods; 1992 Jul; 152(1):89-104. PubMed ID: 1640112
[TBL] [Abstract][Full Text] [Related]
18. Cloning and sequence analysis of cDNAs encoding the heavy and light chain variable regions of a human monoclonal antibody with specificity for hepatitis B surface antigen.
Ryu CJ; Chung HK; Hong HJ
Biochim Biophys Acta; 1998 Apr; 1380(2):151-5. PubMed ID: 9565674
[TBL] [Abstract][Full Text] [Related]
19. Cloning and sequencing of immunoglobulin variable-region genes using degenerate oligodeoxyribonucleotides and polymerase chain reaction.
LeBoeuf RD; Galin FS; Hollinger SK; Peiper SC; Blalock JE
Gene; 1989 Oct; 82(2):371-7. PubMed ID: 2583527
[TBL] [Abstract][Full Text] [Related]
20. Universal cloning and direct sequencing of rearranged antibody V genes using C region primers, biotin-captured cDNA and one-side PCR.
Heinrichs A; Milstein C; Gherardi E
J Immunol Methods; 1995 Jan; 178(2):241-51. PubMed ID: 7836786
[TBL] [Abstract][Full Text] [Related]
[Next] [New Search]
