These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
2. Improved PCR-based gene synthesis method and its application to the Citrobacter freundii phytase gene codon modification. Gordeeva TL; Borschevskaya LN; Sineoky SP J Microbiol Methods; 2010 May; 81(2):147-52. PubMed ID: 20226218 [TBL] [Abstract][Full Text] [Related]
3. Gene synthesis method based on overlap extension PCR and DNAWorks program. Li G; Dong BX; Liu YH; Li CJ; Zhang LP Methods Mol Biol; 2013; 1073():9-17. PubMed ID: 23996435 [TBL] [Abstract][Full Text] [Related]
4. PCR-based accurate synthesis of long DNA sequences. Xiong AS; Yao QH; Peng RH; Duan H; Li X; Fan HQ; Cheng ZM; Li Y Nat Protoc; 2006; 1(2):791-7. PubMed ID: 17406309 [TBL] [Abstract][Full Text] [Related]
5. Overexpression of a synthetic gene encoding human alpha interferon in Escherichia coli. Neves FO; Ho PL; Raw I; Pereira CA; Moreira C; Nascimento AL Protein Expr Purif; 2004 Jun; 35(2):353-9. PubMed ID: 15135413 [TBL] [Abstract][Full Text] [Related]
10. A fluorescence selection method for accurate large-gene synthesis. Kim H; Han H; Shin D; Bang D Chembiochem; 2010 Nov; 11(17):2448-52. PubMed ID: 20981747 [TBL] [Abstract][Full Text] [Related]
11. Cost effectiveness of colony lysis and colony PCR methods for screening of recombinant Escherichia coli colonies--a comparative study. Azhahianambi P; Ghosh S; Ashok Kumar C; Suryanarayana VV Indian J Exp Biol; 2008 Oct; 46(10):731-5. PubMed ID: 19024172 [TBL] [Abstract][Full Text] [Related]
12. Gene synthesis by a LCR-based approach: high-level production of leptin-L54 using synthetic gene in Escherichia coli. Au LC; Yang FY; Yang WJ; Lo SH; Kao CF Biochem Biophys Res Commun; 1998 Jul; 248(1):200-3. PubMed ID: 9675111 [TBL] [Abstract][Full Text] [Related]
13. A simple, universal, efficient PCR-based gene synthesis method: sequential OE-PCR gene synthesis. Zhang P; Ding Y; Liao W; Chen Q; Zhang H; Qi P; He T; Wang J; Deng S; Pan T; Ren H; Pan W Gene; 2013 Jul; 524(2):347-54. PubMed ID: 23597923 [TBL] [Abstract][Full Text] [Related]
14. A PCR-mediated gene synthesis strategy involving the assembly of oligonucleotides representing only one of the strands. Jayaraman K; Puccini CJ Biotechniques; 1992 Mar; 12(3):392-8. PubMed ID: 1571150 [TBL] [Abstract][Full Text] [Related]
15. Efficient site-directed mutagenesis using an overlap extension-PCR method for expressing Mycoplasma hyopneumoniae genes in Escherichia coli. Simionatto S; Marchioro SB; Galli V; Luerce TD; Hartwig DD; Moreira AN; Dellagostin OA J Microbiol Methods; 2009 Oct; 79(1):101-5. PubMed ID: 19733599 [TBL] [Abstract][Full Text] [Related]
16. [PCR-based assembly of the DNA sequence coding for tetanus toxin C fragment]. Yang XQ; Wu JB; Jiang B; Zhao YG; Chen XQ Nan Fang Yi Ke Da Xue Xue Bao; 2008 Mar; 28(3):363-5. PubMed ID: 18359691 [TBL] [Abstract][Full Text] [Related]
17. A Novel Platform for High-Throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli. Sequeira AF; BrĂ¡s JLA; Fernandes VO; Guerreiro CIPD; Vincentelli R; Fontes CMGA Methods Mol Biol; 2017; 1620():113-128. PubMed ID: 28540703 [TBL] [Abstract][Full Text] [Related]
18. A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis. Peng RH; Xiong AS; Yao QH Appl Microbiol Biotechnol; 2006 Nov; 73(1):234-40. PubMed ID: 17021880 [TBL] [Abstract][Full Text] [Related]
19. Quikgene: a gene synthesis method integrated with ligation-free cloning. Mao Y; Lin J; Zhou A; Ji K; Downey JS; Chen R; Han A Anal Biochem; 2011 Aug; 415(1):21-6. PubMed ID: 21530481 [TBL] [Abstract][Full Text] [Related]
20. Polymerase chain reaction-mediated gene synthesis: synthesis of a gene coding for isozyme c of horseradish peroxidase. Jayaraman K; Fingar SA; Shah J; Fyles J Proc Natl Acad Sci U S A; 1991 May; 88(10):4084-8. PubMed ID: 1851991 [TBL] [Abstract][Full Text] [Related] [Next] [New Search]