BIOMARKERS

Molecular Biopsy of Human Tumors

- a resource for Precision Medicine *

174 related articles for article (PubMed ID: 15466573)

  • 1. Family shuffling of expandase genes to enhance substrate specificity for penicillin G.
    Hsu JS; Yang YB; Deng CH; Wei CL; Liaw SH; Tsai YC
    Appl Environ Microbiol; 2004 Oct; 70(10):6257-63. PubMed ID: 15466573
    [TBL] [Abstract][Full Text] [Related]  

  • 2. New strategy of site-directed mutagenesis identifies new sites to improve Streptomyces clavuligerus deacetoxycephalosporin C synthase activity toward penicillin G.
    Ji J; Tian X; Fan K; Yang K
    Appl Microbiol Biotechnol; 2012 Mar; 93(6):2395-401. PubMed ID: 21909691
    [TBL] [Abstract][Full Text] [Related]  

  • 3. A complete library of amino acid alterations at R306 in Streptomyces clavuligerus deacetoxycephalosporin C synthase demonstrates its structural role in the ring-expansion activity.
    Sim Goo K; Song Chua C; Sim TS
    Proteins; 2008 Feb; 70(3):739-47. PubMed ID: 17729280
    [TBL] [Abstract][Full Text] [Related]  

  • 4. Directed evolution of Streptomyces clavuligerus deacetoxycephalosporin C synthase for enhancement of penicillin G expansion.
    Wei CL; Yang YB; Deng CH; Liu WC; Hsu JS; Lin YC; Liaw SH; Tsai YC
    Appl Environ Microbiol; 2005 Dec; 71(12):8873-80. PubMed ID: 16332884
    [TBL] [Abstract][Full Text] [Related]  

  • 5. The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S. clavuligerus.
    Lee HJ; Lloyd MD; Harlos K; Schofield CJ
    Biochem Biophys Res Commun; 2000 Jan; 267(1):445-8. PubMed ID: 10623639
    [TBL] [Abstract][Full Text] [Related]  

  • 6. Iterative combinatorial mutagenesis as an effective strategy for generation of deacetoxycephalosporin C synthase with improved activity toward penicillin G.
    Ji J; Fan K; Tian X; Zhang X; Zhang Y; Yang K
    Appl Environ Microbiol; 2012 Nov; 78(21):7809-12. PubMed ID: 22923414
    [TBL] [Abstract][Full Text] [Related]  

  • 7. Mutation of N304 to leucine in Streptomyces clavuligerus deacetoxycephalosporin C synthase creates an enzyme with increased penicillin analogue conversion.
    Chin HS; Sim J; Sim TS
    Biochem Biophys Res Commun; 2001 Sep; 287(2):507-13. PubMed ID: 11554757
    [TBL] [Abstract][Full Text] [Related]  

  • 8. Cloning and expression of a hybrid Streptomyces clavuligerus cefE gene in Penicillium chrysogenum.
    Cantwell CA; Beckmann RJ; Dotzlaf JE; Fisher DL; Skatrud PL; Yeh WK; Queener SW
    Curr Genet; 1990 Mar; 17(3):213-21. PubMed ID: 2111228
    [TBL] [Abstract][Full Text] [Related]  

  • 9. Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase.
    Kovacevic S; Weigel BJ; Tobin MB; Ingolia TD; Miller JR
    J Bacteriol; 1989 Feb; 171(2):754-60. PubMed ID: 2644235
    [TBL] [Abstract][Full Text] [Related]  

  • 10. The invariant F283 and its strategic position in the hydrophobic cleft of Streptomyces jumonjinensis isopenicillin N synthase active site are functionally important.
    Wong E; Sim J; Sim TS
    Biochem Biophys Res Commun; 2001 May; 283(3):621-6. PubMed ID: 11341769
    [TBL] [Abstract][Full Text] [Related]  

  • 11. The beta-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus.
    Kovacevic S; Tobin MB; Miller JR
    J Bacteriol; 1990 Jul; 172(7):3952-8. PubMed ID: 1694525
    [TBL] [Abstract][Full Text] [Related]  

  • 12. Relevant double mutations in bioengineered Streptomyces clavuligerus deacetoxycephalosporin C synthase result in higher binding specificities which improve penicillin bioconversion.
    Goo KS; Chua CS; Sim TS
    Appl Environ Microbiol; 2008 Feb; 74(4):1167-75. PubMed ID: 18083859
    [TBL] [Abstract][Full Text] [Related]  

  • 13. Deacetoxycephalosporin C synthase isozymes exhibit diverse catalytic activity and substrate specificity.
    Chin HS; Sim J; Seah KI; Sim TS
    FEMS Microbiol Lett; 2003 Jan; 218(2):251-7. PubMed ID: 12586400
    [TBL] [Abstract][Full Text] [Related]  

  • 14. Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin G.
    Gao Q; Piret JM; Adrio JL; Demain AL
    J Ind Microbiol Biotechnol; 2003 Mar; 30(3):190-4. PubMed ID: 12715257
    [TBL] [Abstract][Full Text] [Related]  

  • 15. Directed evolution and rational approaches to improving Streptomyces clavuligerus deacetoxycephalosporin C synthase for cephalosporin production.
    Goo KS; Chua CS; Sim TS
    J Ind Microbiol Biotechnol; 2009 May; 36(5):619-33. PubMed ID: 19277744
    [TBL] [Abstract][Full Text] [Related]  

  • 16. Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus.
    Dotzlaf JE; Yeh WK
    J Biol Chem; 1989 Jun; 264(17):10219-27. PubMed ID: 2656705
    [TBL] [Abstract][Full Text] [Related]  

  • 17. Continuous cultivations of a Penicillium chrysogenum strain expressing the expandase gene from Streptomyces clavuligerus: Kinetics of adipoyl-7-aminodeacetoxycephalosporanic acid and byproduct formations.
    Robin J; Bruheim P; Nielsen ML; Noorman H; Nielsen J
    Biotechnol Bioeng; 2003 Aug; 83(3):353-60. PubMed ID: 12783491
    [TBL] [Abstract][Full Text] [Related]  

  • 18. Conformational flexibility of the C terminus with implications for substrate binding and catalysis revealed in a new crystal form of deacetoxycephalosporin C synthase.
    Oster LM; van Scheltinga AC; Valegård K; Hose AM; Dubus A; Hajdu J; Andersson I
    J Mol Biol; 2004 Oct; 343(1):157-71. PubMed ID: 15381427
    [TBL] [Abstract][Full Text] [Related]  

  • 19. [Evaluation of penicillin expandase mutants and complex substrate inhibition characteristics at high concentrations of penicillin G].
    Wu L; Fan K; Ji J; Yang K
    Sheng Wu Gong Cheng Xue Bao; 2015 Dec; 31(12):1690-9. PubMed ID: 27093832
    [TBL] [Abstract][Full Text] [Related]  

  • 20. The structural basis of cephalosporin formation in a mononuclear ferrous enzyme.
    Valegård K; Terwisscha van Scheltinga AC; Dubus A; Ranghino G; Oster LM; Hajdu J; Andersson I
    Nat Struct Mol Biol; 2004 Jan; 11(1):95-101. PubMed ID: 14718929
    [TBL] [Abstract][Full Text] [Related]  

    [Next]    [New Search]
    of 9.