281 related articles for article (PubMed ID: 15479237)
21. Preparation of multiple site-specific mutant proteins for NMR studies by PCR-directed cell-free protein synthesis.
Ozawa K; Qi R
Methods Mol Biol; 2014; 1118():169-87. PubMed ID: 24395416
[TBL] [Abstract][Full Text] [Related]
22. Amino acid-selective isotope labeling of proteins for nuclear magnetic resonance study: proteins secreted by Brevibacillus choshinensis.
Tanio M; Tanaka R; Tanaka T; Kohno T
Anal Biochem; 2009 Mar; 386(2):156-60. PubMed ID: 19161967
[TBL] [Abstract][Full Text] [Related]
23. A novel method for selective isotope labeling of bacterially expressed proteins.
Lee KM; Androphy EJ; Baleja JD
J Biomol NMR; 1995 Jan; 5(1):93-6. PubMed ID: 7881274
[TBL] [Abstract][Full Text] [Related]
24. Escherichia coli strain for thermoinducible T7 RNA polymerase-driven expression.
Fedorova ND; Peredelchuk MY; Kirpichnikov MP; Bennett GN
Gene; 1996 Oct; 177(1-2):267-8. PubMed ID: 8921879
[TBL] [Abstract][Full Text] [Related]
25. Overproduction of the toxic protein, bovine pancreatic DNaseI, in Escherichia coli using a tightly controlled T7-promoter-based vector.
Doherty AJ; Connolly BA; Worrall AF
Gene; 1993 Dec; 136(1-2):337-40. PubMed ID: 8294027
[TBL] [Abstract][Full Text] [Related]
26. Selective labeling and unlabeling strategies in protein solid-state NMR spectroscopy.
Lacabanne D; Meier BH; Böckmann A
J Biomol NMR; 2018 Jul; 71(3):141-150. PubMed ID: 29197975
[TBL] [Abstract][Full Text] [Related]
27. Fast structure-based assignment of 15N HSQC spectra of selectively 15N-labeled paramagnetic proteins.
Pintacuda G; Keniry MA; Huber T; Park AY; Dixon NE; Otting G
J Am Chem Soc; 2004 Mar; 126(9):2963-70. PubMed ID: 14995214
[TBL] [Abstract][Full Text] [Related]
28. Cell-free membrane protein expression for solid-state NMR.
Abdine A; Park KH; Warschawski DE
Methods Mol Biol; 2012; 831():85-109. PubMed ID: 22167670
[TBL] [Abstract][Full Text] [Related]
29. In vivo incorporation of unnatural amino acids to probe structure, dynamics, and ligand binding in a large protein by nuclear magnetic resonance spectroscopy.
Cellitti SE; Jones DH; Lagpacan L; Hao X; Zhang Q; Hu H; Brittain SM; Brinker A; Caldwell J; Bursulaya B; Spraggon G; Brock A; Ryu Y; Uno T; Schultz PG; Geierstanger BH
J Am Chem Soc; 2008 Jul; 130(29):9268-81. PubMed ID: 18576636
[TBL] [Abstract][Full Text] [Related]
30. Escherichia coli cell-free protein synthesis and isotope labeling of mammalian proteins.
Terada T; Yokoyama S
Methods Enzymol; 2015; 565():311-45. PubMed ID: 26577737
[TBL] [Abstract][Full Text] [Related]
31. Efficient production of isotopically labeled proteins by cell-free synthesis: a practical protocol.
Torizawa T; Shimizu M; Taoka M; Miyano H; Kainosho M
J Biomol NMR; 2004 Nov; 30(3):311-25. PubMed ID: 15754057
[TBL] [Abstract][Full Text] [Related]
32. Stringent regulation and high-level expression of heterologous genes in Escherichia coli using T7 system controllable by the araBAD promoter.
Chao YP; Chiang CJ; Hung WB
Biotechnol Prog; 2002; 18(2):394-400. PubMed ID: 11934312
[TBL] [Abstract][Full Text] [Related]
33. Multiple-site labeling of proteins with unnatural amino acids.
Loscha KV; Herlt AJ; Qi R; Huber T; Ozawa K; Otting G
Angew Chem Int Ed Engl; 2012 Feb; 51(9):2243-6. PubMed ID: 22298420
[No Abstract] [Full Text] [Related]
34. Increasing PCR fragment stability and protein yields in a cell-free system with genetically modified Escherichia coli extracts.
Michel-Reydellet N; Woodrow K; Swartz J
J Mol Microbiol Biotechnol; 2005; 9(1):26-34. PubMed ID: 16254443
[TBL] [Abstract][Full Text] [Related]
35. Cell-free protein synthesis in an autoinduction system for NMR studies of protein-protein interactions.
Ozawa K; Jergic S; Crowther JA; Thompson PR; Wijffels G; Otting G; Dixon NA
J Biomol NMR; 2005 Jul; 32(3):235-41. PubMed ID: 16132823
[TBL] [Abstract][Full Text] [Related]
36. Site-specific incorporation of unnatural amino acids into proteins by cell-free protein synthesis.
Ozawa K; Loh CT
Methods Mol Biol; 2014; 1118():189-203. PubMed ID: 24395417
[TBL] [Abstract][Full Text] [Related]
37. Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O.
Su XC; Loh CT; Qi R; Otting G
J Biomol NMR; 2011 May; 50(1):35-42. PubMed ID: 21318579
[TBL] [Abstract][Full Text] [Related]
38. [Problems of genetic enzymology].
Kiselev LL; Engelhardt VA
Mol Biol (Mosk); 1977; 11(6):1292-1303. PubMed ID: 88007
[No Abstract] [Full Text] [Related]
39. NMR analysis of in vitro-synthesized proteins without purification: a high-throughput approach.
Guignard L; Ozawa K; Pursglove SE; Otting G; Dixon NE
FEBS Lett; 2002 Jul; 524(1-3):159-62. PubMed ID: 12135760
[TBL] [Abstract][Full Text] [Related]
40. A coupled in vitro transcription-translation system for the exclusive synthesis of polypeptides expressed from the T7 promoter.
Nevin DE; Pratt JM
FEBS Lett; 1991 Oct; 291(2):259-63. PubMed ID: 1936272
[TBL] [Abstract][Full Text] [Related]
[Previous] [Next] [New Search]