These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


BIOMARKERS

Molecular Biopsy of Human Tumors

- a resource for Precision Medicine *

147 related articles for article (PubMed ID: 16054701)

  • 1. Coupled analysis of bacterial transformants and ligation mixture by duplex PCR enables detection of fatal instability of a nascent recombinant plasmid.
    Al-Allaf FA; Tolmachov O; Themis M; Coutelle C
    J Biochem Biophys Methods; 2005 Aug; 64(2):142-6. PubMed ID: 16054701
    [TBL] [Abstract][Full Text] [Related]  

  • 2. A PCR-after-ligation method for cloning of multiple DNA inserts.
    An Y; Wu W; Lv A
    Anal Biochem; 2010 Jul; 402(2):203-5. PubMed ID: 20363207
    [TBL] [Abstract][Full Text] [Related]  

  • 3. Identification and characterization of IS1 transposition in plasmid amplification mutants of E. coli clones producing DNA vaccines.
    Prather KL; Edmonds MC; Herod JW
    Appl Microbiol Biotechnol; 2006 Dec; 73(4):815-26. PubMed ID: 16941177
    [TBL] [Abstract][Full Text] [Related]  

  • 4. Restoration of gene function by homologous recombination: from PCR to gene expression in one step.
    Yosef I; Bloushtain N; Shapira M; Qimron U
    Appl Environ Microbiol; 2004 Dec; 70(12):7156-60. PubMed ID: 15574912
    [TBL] [Abstract][Full Text] [Related]  

  • 5. A method for generating sticky-end PCR products which facilitates unidirectional cloning and the one-step assembly of complex DNA constructs.
    Walker A; Taylor J; Rowe D; Summers D
    Plasmid; 2008 May; 59(3):155-62. PubMed ID: 18395798
    [TBL] [Abstract][Full Text] [Related]  

  • 6. An improved method for fast, robust, and seamless integration of DNA fragments into multiple plasmids.
    Benoit RM; Wilhelm RN; Scherer-Becker D; Ostermeier C
    Protein Expr Purif; 2006 Jan; 45(1):66-71. PubMed ID: 16289702
    [TBL] [Abstract][Full Text] [Related]  

  • 7. An efficient plasmid vector for expression cloning of large numbers of PCR fragments in Escherichia coli.
    Reisinger C; Kern A; Fesko K; Schwab H
    Appl Microbiol Biotechnol; 2007 Nov; 77(1):241-4. PubMed ID: 17786428
    [TBL] [Abstract][Full Text] [Related]  

  • 8. The "Frankenplasmid" lab: an investigative exercise for teaching recombinant DNA methods.
    Dean DM; Wilder JA
    Biochem Mol Biol Educ; 2011; 39(5):367-74. PubMed ID: 21948510
    [TBL] [Abstract][Full Text] [Related]  

  • 9. SCALEs: multiscale analysis of library enrichment.
    Lynch MD; Warnecke T; Gill RT
    Nat Methods; 2007 Jan; 4(1):87-93. PubMed ID: 17099705
    [TBL] [Abstract][Full Text] [Related]  

  • 10. An optimized recipe for cloning of the polymerase chain reaction-amplified DNA inserts into plasmid vectors.
    Topcu Z
    Acta Biochim Pol; 2000; 47(3):841-6. PubMed ID: 11310983
    [TBL] [Abstract][Full Text] [Related]  

  • 11. A noncommercial polymerase chain reaction-based method to approach one hundred percent recombinant clone selection efficiency.
    Shareef MM; Dancea HC; Gross JL; Myers TT; Griggs WW; Ahmed MM; Sheldon DG
    Anal Biochem; 2008 Nov; 382(1):75-6. PubMed ID: 18674510
    [TBL] [Abstract][Full Text] [Related]  

  • 12. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC.
    Li MZ; Elledge SJ
    Nat Methods; 2007 Mar; 4(3):251-6. PubMed ID: 17293868
    [TBL] [Abstract][Full Text] [Related]  

  • 13. DNA cloning by homologous recombination in Escherichia coli.
    Zhang Y; Muyrers JP; Testa G; Stewart AF
    Nat Biotechnol; 2000 Dec; 18(12):1314-7. PubMed ID: 11101815
    [TBL] [Abstract][Full Text] [Related]  

  • 14. Direct cloning of the unknown flanking DNA fragments from a large insert without restriction mapping.
    Song BL; Qi W; Li BL
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai); 2002 May; 34(3):365-8. PubMed ID: 12019453
    [TBL] [Abstract][Full Text] [Related]  

  • 15. Cost effectiveness of colony lysis and colony PCR methods for screening of recombinant Escherichia coli colonies--a comparative study.
    Azhahianambi P; Ghosh S; Ashok Kumar C; Suryanarayana VV
    Indian J Exp Biol; 2008 Oct; 46(10):731-5. PubMed ID: 19024172
    [TBL] [Abstract][Full Text] [Related]  

  • 16. Rapid one-step characterization of recombinant vectors by direct analysis of transformed Escherichia coli colonies.
    Sandhu GS; Precup JW; Kline BC
    Biotechniques; 1989; 7(7):689-90. PubMed ID: 2517212
    [TBL] [Abstract][Full Text] [Related]  

  • 17. Short description of an alternative simplified method for screening recombinant clones within the "AdEasy-System" by Duplex-PCR.
    Antolovic D; Koch M; Bohlmann I; Kienle P; Büchler M; Weitz J
    BMC Biotechnol; 2005 Jan; 5():1. PubMed ID: 15642126
    [TBL] [Abstract][Full Text] [Related]  

  • 18. Electrotransformation of Thermoanaerobacter ethanolicus JW200.
    Peng H; Fu B; Mao Z; Shao W
    Biotechnol Lett; 2006 Dec; 28(23):1913-7. PubMed ID: 16988780
    [TBL] [Abstract][Full Text] [Related]  

  • 19. From Gateway to MultiSite Gateway in one recombination event.
    Magnani E; Bartling L; Hake S
    BMC Mol Biol; 2006 Dec; 7():46. PubMed ID: 17150110
    [TBL] [Abstract][Full Text] [Related]  

  • 20. Development of an antibiotic-free plasmid selection system based on glycine auxotrophy for recombinant protein overproduction in Escherichia coli.
    Vidal L; Pinsach J; Striedner G; Caminal G; Ferrer P
    J Biotechnol; 2008 Mar; 134(1-2):127-36. PubMed ID: 18308411
    [TBL] [Abstract][Full Text] [Related]  

    [Next]    [New Search]
    of 8.