These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
4. Efficient generation of recessive traits in diploid sake yeast by targeted gene disruption and loss of heterozygosity. Kotaka A; Sahara H; Kondo A; Ueda M; Hata Y Appl Microbiol Biotechnol; 2009 Feb; 82(2):387-95. PubMed ID: 19137286 [TBL] [Abstract][Full Text] [Related]
5. Study Essential Gene Functions by Plasmid Shuffling. Fan L; Xiao W Methods Mol Biol; 2021; 2196():53-62. PubMed ID: 32889712 [TBL] [Abstract][Full Text] [Related]
6. An improved integration replacement/disruption method for mutagenesis of yeast essential genes. Tohe A; Oguchi T Genes Genet Syst; 2000 Feb; 75(1):33-9. PubMed ID: 10846619 [TBL] [Abstract][Full Text] [Related]
7. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Boeke JD; Trueheart J; Natsoulis G; Fink GR Methods Enzymol; 1987; 154():164-75. PubMed ID: 3323810 [TBL] [Abstract][Full Text] [Related]
8. Systematic exploration of essential yeast gene function with temperature-sensitive mutants. Li Z; Vizeacoumar FJ; Bahr S; Li J; Warringer J; Vizeacoumar FS; Min R; Vandersluis B; Bellay J; Devit M; Fleming JA; Stephens A; Haase J; Lin ZY; Baryshnikova A; Lu H; Yan Z; Jin K; Barker S; Datti A; Giaever G; Nislow C; Bulawa C; Myers CL; Costanzo M; Gingras AC; Zhang Z; Blomberg A; Bloom K; Andrews B; Boone C Nat Biotechnol; 2011 Apr; 29(4):361-7. PubMed ID: 21441928 [TBL] [Abstract][Full Text] [Related]
9. Marker-disruptive gene integration and URA3 recycling for multiple gene manipulation in Saccharomyces cerevisiae. Kaneko S; Tanaka T; Noda H; Fukuda H; Akada R; Kondo A Appl Microbiol Biotechnol; 2009 Jun; 83(4):783-9. PubMed ID: 19455322 [TBL] [Abstract][Full Text] [Related]
10. Allele-specific gene targeting in Candida albicans results from heterology between alleles. Yesland K; Fonzi WA Microbiology (Reading); 2000 Sep; 146 ( Pt 9)():2097-2104. PubMed ID: 10974097 [TBL] [Abstract][Full Text] [Related]
11. Two Drosophila melanogaster mutations block successive steps of de novo purine synthesis. Henikoff S; Nash D; Hards R; Bleskan J; Woolford JF; Naguib F; Patterson D Proc Natl Acad Sci U S A; 1986 Jun; 83(11):3919-23. PubMed ID: 3086869 [TBL] [Abstract][Full Text] [Related]
12. A novel method for the cloning of chromosomal mutations in a single step: isolation of two mutant alleles of envZ, an osmoregulatory gene from Escherichia coli. Comeau DE; Inouye M Mol Gen Genet; 1988 Jul; 213(1):166-9. PubMed ID: 2851704 [TBL] [Abstract][Full Text] [Related]
14. A two-step method for the introduction of single or multiple defined point mutations into the genome of Saccharomyces cerevisiae. Toulmay A; Schneiter R Yeast; 2006 Aug; 23(11):825-31. PubMed ID: 16921548 [TBL] [Abstract][Full Text] [Related]
16. An Updated Collection of Sequence Barcoded Temperature-Sensitive Alleles of Yeast Essential Genes. Kofoed M; Milbury KL; Chiang JH; Sinha S; Ben-Aroya S; Giaever G; Nislow C; Hieter P; Stirling PC G3 (Bethesda); 2015 Jul; 5(9):1879-87. PubMed ID: 26175450 [TBL] [Abstract][Full Text] [Related]
17. The SAT1 flipper, an optimized tool for gene disruption in Candida albicans. Reuss O; Vik A; Kolter R; Morschhäuser J Gene; 2004 Oct; 341():119-27. PubMed ID: 15474295 [TBL] [Abstract][Full Text] [Related]
18. [Development of a genetic transformation system for Candida tropicalis based on a reusable selection marker of URA3 gene]. Xiang Z; Chen X; Zhang L; Shen W; Fan Y; Lu M Yi Chuan; 2014 Oct; 36(10):1053-61. PubMed ID: 25406254 [TBL] [Abstract][Full Text] [Related]
19. A double counter-selection system for the study of null alleles of essential genes in Escherichia coli. Murphy CK; Stewart EJ; Beckwith J Gene; 1995 Mar; 155(1):1-7. PubMed ID: 7698651 [TBL] [Abstract][Full Text] [Related]