267 related articles for article (PubMed ID: 16971398)
1. Improving solubility and refolding efficiency of human V(H)s by a novel mutational approach.
Tanha J; Nguyen TD; Ng A; Ryan S; Ni F; Mackenzie R
Protein Eng Des Sel; 2006 Nov; 19(11):503-9. PubMed ID: 16971398
[TBL] [Abstract][Full Text] [Related]
2. Aggregation-resistant VHs selected by in vitro evolution tend to have disulfide-bonded loops and acidic isoelectric points.
Arbabi-Ghahroudi M; To R; Gaudette N; Hirama T; Ding W; MacKenzie R; Tanha J
Protein Eng Des Sel; 2009 Feb; 22(2):59-66. PubMed ID: 19033278
[TBL] [Abstract][Full Text] [Related]
3. The influence of the framework core residues on the biophysical properties of immunoglobulin heavy chain variable domains.
Honegger A; Malebranche AD; Röthlisberger D; Plückthun A
Protein Eng Des Sel; 2009 Mar; 22(3):121-34. PubMed ID: 19136675
[TBL] [Abstract][Full Text] [Related]
4. Crystal structure of HEL4, a soluble, refoldable human V(H) single domain with a germ-line scaffold.
Jespers L; Schon O; James LC; Veprintsev D; Winter G
J Mol Biol; 2004 Apr; 337(4):893-903. PubMed ID: 15033359
[TBL] [Abstract][Full Text] [Related]
5. The importance of framework residues H6, H7 and H10 in antibody heavy chains: experimental evidence for a new structural subclassification of antibody V(H) domains.
Jung S; Spinelli S; Schimmele B; Honegger A; Pugliese L; Cambillau C; Plückthun A
J Mol Biol; 2001 Jun; 309(3):701-16. PubMed ID: 11397090
[TBL] [Abstract][Full Text] [Related]
6. Structure-based improvement of the biophysical properties of immunoglobulin VH domains with a generalizable approach.
Ewert S; Honegger A; Plückthun A
Biochemistry; 2003 Feb; 42(6):1517-28. PubMed ID: 12578364
[TBL] [Abstract][Full Text] [Related]
7. Molecular dynamics simulations highlight mobile regions in proteins: A novel suggestion for converting a murine V(H) domain into a more tractable species.
Voordijk S; Hansson T; Hilvert D; van Gunsteren WF
J Mol Biol; 2000 Jul; 300(4):963-73. PubMed ID: 10891281
[TBL] [Abstract][Full Text] [Related]
8. Affinity maturation of a V(H)H by mutational hotspot randomization.
Yau KY; Dubuc G; Li S; Hirama T; Mackenzie CR; Jermutus L; Hall JC; Tanha J
J Immunol Methods; 2005 Feb; 297(1-2):213-24. PubMed ID: 15777944
[TBL] [Abstract][Full Text] [Related]
9. Stabilization and humanization of a single-chain Fv antibody fragment specific for human lymphocyte antigen CD19 by designed point mutations and CDR-grafting onto a human framework.
Kügler M; Stein C; Schwenkert M; Saul D; Vockentanz L; Huber T; Wetzel SK; Scholz O; Plückthun A; Honegger A; Fey GH
Protein Eng Des Sel; 2009 Mar; 22(3):135-47. PubMed ID: 19188138
[TBL] [Abstract][Full Text] [Related]
10. Intrabody construction and expression III: engineering hyperstable V(H) domains.
Wirtz P; Steipe B
Protein Sci; 1999 Nov; 8(11):2245-50. PubMed ID: 10595527
[TBL] [Abstract][Full Text] [Related]
11. Antibody engineering reveals the important role of J segments in the production efficiency of llama single-domain antibodies in Saccharomyces cerevisiae.
Gorlani A; Hulsik DL; Adams H; Vriend G; Hermans P; Verrips T
Protein Eng Des Sel; 2012 Jan; 25(1):39-46. PubMed ID: 22143875
[TBL] [Abstract][Full Text] [Related]
12. Contributions of a highly conserved VH/VL hydrogen bonding interaction to scFv folding stability and refolding efficiency.
Tan PH; Sandmaier BM; Stayton PS
Biophys J; 1998 Sep; 75(3):1473-82. PubMed ID: 9726949
[TBL] [Abstract][Full Text] [Related]
13. Selection and expression of recombinant single domain antibodies from a hyper-immunized library against the hapten azoxystrobin.
Makvandi-Nejad S; Fjällman T; Arbabi-Ghahroudi M; MacKenzie CR; Hall JC
J Immunol Methods; 2011 Oct; 373(1-2):8-18. PubMed ID: 21777591
[TBL] [Abstract][Full Text] [Related]
14. Analysis of the horse V(H) repertoire and comparison with the human IGHV germline genes, and sheep, cattle and pig V(H) sequences.
Almagro JC; Martinez L; Smith SL; Alagon A; Estevez J; Paniagua J
Mol Immunol; 2006 Apr; 43(11):1836-45. PubMed ID: 16337682
[TBL] [Abstract][Full Text] [Related]
15. Disulfide linkage engineering for improving biophysical properties of human VH domains.
Kim DY; Kandalaft H; Ding W; Ryan S; van Faassen H; Hirama T; Foote SJ; MacKenzie R; Tanha J
Protein Eng Des Sel; 2012 Oct; 25(10):581-9. PubMed ID: 22942392
[TBL] [Abstract][Full Text] [Related]
16. Conserved amino acid networks involved in antibody variable domain interactions.
Wang N; Smith WF; Miller BR; Aivazian D; Lugovskoy AA; Reff ME; Glaser SM; Croner LJ; Demarest SJ
Proteins; 2009 Jul; 76(1):99-114. PubMed ID: 19089973
[TBL] [Abstract][Full Text] [Related]
17. Thermal unfolding of a llama antibody fragment: a two-state reversible process.
Pérez JM; Renisio JG; Prompers JJ; van Platerink CJ; Cambillau C; Darbon H; Frenken LG
Biochemistry; 2001 Jan; 40(1):74-83. PubMed ID: 11141058
[TBL] [Abstract][Full Text] [Related]
18. Expression and production of llama variable heavy-chain antibody fragments (V(HH)s) by Aspergillus awamori.
Joosten V; Gouka RJ; van den Hondel CA; Verrips CT; Lokman BC
Appl Microbiol Biotechnol; 2005 Jan; 66(4):384-92. PubMed ID: 15378291
[TBL] [Abstract][Full Text] [Related]
19. Construction of a large phage-displayed human antibody domain library with a scaffold based on a newly identified highly soluble, stable heavy chain variable domain.
Chen W; Zhu Z; Feng Y; Xiao X; Dimitrov DS
J Mol Biol; 2008 Oct; 382(3):779-89. PubMed ID: 18687338
[TBL] [Abstract][Full Text] [Related]
20. Disulfide bond introduction for general stabilization of immunoglobulin heavy-chain variable domains.
Saerens D; Conrath K; Govaert J; Muyldermans S
J Mol Biol; 2008 Mar; 377(2):478-88. PubMed ID: 18262543
[TBL] [Abstract][Full Text] [Related]
[Next] [New Search]
