These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


BIOMARKERS

Molecular Biopsy of Human Tumors

- a resource for Precision Medicine *

203 related articles for article (PubMed ID: 17595310)

  • 1. Efficient site-directed saturation mutagenesis using degenerate oligonucleotides.
    Steffens DL; Williams JG
    J Biomol Tech; 2007 Jul; 18(3):147-9. PubMed ID: 17595310
    [TBL] [Abstract][Full Text] [Related]  

  • 2. Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit.
    Hogrefe HH; Cline J; Youngblood GL; Allen RM
    Biotechniques; 2002 Nov; 33(5):1158-60, 1162, 1164-5. PubMed ID: 12449398
    [TBL] [Abstract][Full Text] [Related]  

  • 3. A rapid, efficient, and economical inverse polymerase chain reaction-based method for generating a site saturation mutant library.
    Jain PC; Varadarajan R
    Anal Biochem; 2014 Mar; 449():90-8. PubMed ID: 24333246
    [TBL] [Abstract][Full Text] [Related]  

  • 4. Construction of "small-intelligent" focused mutagenesis libraries using well-designed combinatorial degenerate primers.
    Tang L; Gao H; Zhu X; Wang X; Zhou M; Jiang R
    Biotechniques; 2012 Mar; 52(3):149-58. PubMed ID: 22401547
    [TBL] [Abstract][Full Text] [Related]  

  • 5. An efficient approach for site-directed mutagenesis using central overlapping primers.
    Wang H; Zhou N; Ding F; Li Z; Chen R; Han A; Liu R
    Anal Biochem; 2011 Nov; 418(2):304-6. PubMed ID: 21803022
    [TBL] [Abstract][Full Text] [Related]  

  • 6. Thermostable DNA ligase-mediated PCR production of circular plasmid (PPCP) and its application in directed evolution via in situ error-prone PCR.
    Le Y; Chen H; Zagursky R; Wu JH; Shao W
    DNA Res; 2013 Aug; 20(4):375-82. PubMed ID: 23633530
    [TBL] [Abstract][Full Text] [Related]  

  • 7. Whole plasmid mutagenic PCR for directed protein evolution.
    Matsumura I; Rowe LA
    Biomol Eng; 2005 Jun; 22(1-3):73-9. PubMed ID: 15857786
    [TBL] [Abstract][Full Text] [Related]  

  • 8. A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method.
    Tseng WC; Lin JW; Wei TY; Fang TY
    Anal Biochem; 2008 Apr; 375(2):376-8. PubMed ID: 18198125
    [TBL] [Abstract][Full Text] [Related]  

  • 9. Rapid sequence scanning mutagenesis using in silico oligo design and the Megaprimer PCR of whole plasmid method (MegaWHOP).
    Krauss U; Jaeger KE; Eggert T
    Methods Mol Biol; 2010; 634():127-35. PubMed ID: 20676980
    [TBL] [Abstract][Full Text] [Related]  

  • 10. Mutation Maker, An Open Source Oligo Design Platform for Protein Engineering.
    Hiraga K; Mejzlik P; Marcisin M; Vostrosablin N; Gromek A; Arnold J; Wiewiora S; Svarba R; Prihoda D; Clarova K; Klempir O; Navratil J; Tupa O; Vazquez-Otero A; Walas MW; Holy L; Spale M; Kotowski J; Dzamba D; Temesi G; Russell JH; Marshall NM; Murphy GS; Bitton DA
    ACS Synth Biol; 2021 Feb; 10(2):357-370. PubMed ID: 33433999
    [TBL] [Abstract][Full Text] [Related]  

  • 11. Site-directed mutagenesis.
    Bachman J
    Methods Enzymol; 2013; 529():241-8. PubMed ID: 24011050
    [TBL] [Abstract][Full Text] [Related]  

  • 12. An efficient one-step site-directed and site-saturation mutagenesis protocol.
    Zheng L; Baumann U; Reymond JL
    Nucleic Acids Res; 2004 Aug; 32(14):e115. PubMed ID: 15304544
    [TBL] [Abstract][Full Text] [Related]  

  • 13. Oligonucleotide design and optimized protocol for site-directed mutagenesis.
    Piechocki MP; Hines RN
    Biotechniques; 1994 Apr; 16(4):702-7. PubMed ID: 8024793
    [TBL] [Abstract][Full Text] [Related]  

  • 14. Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates.
    Sanchis J; Fernández L; Carballeira JD; Drone J; Gumulya Y; Höbenreich H; Kahakeaw D; Kille S; Lohmer R; Peyralans JJ; Podtetenieff J; Prasad S; Soni P; Taglieber A; Wu S; Zilly FE; Reetz MT
    Appl Microbiol Biotechnol; 2008 Nov; 81(2):387-97. PubMed ID: 18820909
    [TBL] [Abstract][Full Text] [Related]  

  • 15. New insights into the QuikChange™ process guide the use of Phusion DNA polymerase for site-directed mutagenesis.
    Xia Y; Chu W; Qi Q; Xun L
    Nucleic Acids Res; 2015 Jan; 43(2):e12. PubMed ID: 25399421
    [TBL] [Abstract][Full Text] [Related]  

  • 16. Mutagenesis and functional selection protocols for directed evolution of proteins in E. coli.
    Troll C; Alexander D; Allen J; Marquette J; Camps M
    J Vis Exp; 2011 Mar; (49):. PubMed ID: 21445044
    [TBL] [Abstract][Full Text] [Related]  

  • 17. A method for multi-site-directed mutagenesis based on homologous recombination.
    Liang X; Peng L; Li K; Peterson T; Katzen F
    Anal Biochem; 2012 Aug; 427(1):99-101. PubMed ID: 22579595
    [TBL] [Abstract][Full Text] [Related]  

  • 18. Fine-tuning enzyme activity through saturation mutagenesis.
    Hogrefe HH
    Methods Mol Biol; 2010; 634():271-83. PubMed ID: 20676991
    [TBL] [Abstract][Full Text] [Related]  

  • 19. Targeted amplification of mutant strands for efficient site-directed mutagenesis and mutant screening.
    Young L; Dong Q
    Methods Mol Biol; 2010; 634():147-55. PubMed ID: 20676982
    [TBL] [Abstract][Full Text] [Related]  

  • 20. DNA gap repair in Escherichia coli for multiplex site-directed mutagenesis.
    Lyozin GT; Brunelli L
    FASEB J; 2020 May; 34(5):6351-6368. PubMed ID: 32167210
    [TBL] [Abstract][Full Text] [Related]  

    [Next]    [New Search]
    of 11.