226 related articles for article (PubMed ID: 19277744)
1. Directed evolution and rational approaches to improving Streptomyces clavuligerus deacetoxycephalosporin C synthase for cephalosporin production.
Goo KS; Chua CS; Sim TS
J Ind Microbiol Biotechnol; 2009 May; 36(5):619-33. PubMed ID: 19277744
[TBL] [Abstract][Full Text] [Related]
2. New strategy of site-directed mutagenesis identifies new sites to improve Streptomyces clavuligerus deacetoxycephalosporin C synthase activity toward penicillin G.
Ji J; Tian X; Fan K; Yang K
Appl Microbiol Biotechnol; 2012 Mar; 93(6):2395-401. PubMed ID: 21909691
[TBL] [Abstract][Full Text] [Related]
3. A complete library of amino acid alterations at R306 in Streptomyces clavuligerus deacetoxycephalosporin C synthase demonstrates its structural role in the ring-expansion activity.
Sim Goo K; Song Chua C; Sim TS
Proteins; 2008 Feb; 70(3):739-47. PubMed ID: 17729280
[TBL] [Abstract][Full Text] [Related]
4. Directed evolution of Streptomyces clavuligerus deacetoxycephalosporin C synthase for enhancement of penicillin G expansion.
Wei CL; Yang YB; Deng CH; Liu WC; Hsu JS; Lin YC; Liaw SH; Tsai YC
Appl Environ Microbiol; 2005 Dec; 71(12):8873-80. PubMed ID: 16332884
[TBL] [Abstract][Full Text] [Related]
5. Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase.
Lloyd MD; Lipscomb SJ; Hewitson KS; Hensgens CM; Baldwin JE; Schofield CJ
J Biol Chem; 2004 Apr; 279(15):15420-6. PubMed ID: 14734549
[TBL] [Abstract][Full Text] [Related]
6. Relevant double mutations in bioengineered Streptomyces clavuligerus deacetoxycephalosporin C synthase result in higher binding specificities which improve penicillin bioconversion.
Goo KS; Chua CS; Sim TS
Appl Environ Microbiol; 2008 Feb; 74(4):1167-75. PubMed ID: 18083859
[TBL] [Abstract][Full Text] [Related]
7. Conformational flexibility of the C terminus with implications for substrate binding and catalysis revealed in a new crystal form of deacetoxycephalosporin C synthase.
Oster LM; van Scheltinga AC; Valegård K; Hose AM; Dubus A; Hajdu J; Andersson I
J Mol Biol; 2004 Oct; 343(1):157-71. PubMed ID: 15381427
[TBL] [Abstract][Full Text] [Related]
8. Iterative combinatorial mutagenesis as an effective strategy for generation of deacetoxycephalosporin C synthase with improved activity toward penicillin G.
Ji J; Fan K; Tian X; Zhang X; Zhang Y; Yang K
Appl Environ Microbiol; 2012 Nov; 78(21):7809-12. PubMed ID: 22923414
[TBL] [Abstract][Full Text] [Related]
9. A complete library of amino acid alterations at N304 in Streptomyces clavuligerus deacetoxycephalosporin C synthase elucidates the basis for enhanced penicillin analogue conversion.
Chin HS; Goo KS; Sim TS
Appl Environ Microbiol; 2004 Jan; 70(1):607-9. PubMed ID: 14711695
[TBL] [Abstract][Full Text] [Related]
10. Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS).
Lee HJ; Lloyd MD; Harlos K; Clifton IJ; Baldwin JE; Schofield CJ
J Mol Biol; 2001 May; 308(5):937-48. PubMed ID: 11352583
[TBL] [Abstract][Full Text] [Related]
11. Family shuffling of expandase genes to enhance substrate specificity for penicillin G.
Hsu JS; Yang YB; Deng CH; Wei CL; Liaw SH; Tsai YC
Appl Environ Microbiol; 2004 Oct; 70(10):6257-63. PubMed ID: 15466573
[TBL] [Abstract][Full Text] [Related]
12. The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S. clavuligerus.
Lee HJ; Lloyd MD; Harlos K; Schofield CJ
Biochem Biophys Res Commun; 2000 Jan; 267(1):445-8. PubMed ID: 10623639
[TBL] [Abstract][Full Text] [Related]
13. Mutational evidence supporting the involvement of tripartite residues His183, Asp185, and His243 in Streptomyces clavuligerus deacetoxycephalosporin C synthase for catalysis.
Sim J; Sim TS
Biosci Biotechnol Biochem; 2000 Apr; 64(4):828-32. PubMed ID: 10830499
[TBL] [Abstract][Full Text] [Related]
14. Deacetoxycephalosporin C synthase isozymes exhibit diverse catalytic activity and substrate specificity.
Chin HS; Sim J; Seah KI; Sim TS
FEMS Microbiol Lett; 2003 Jan; 218(2):251-7. PubMed ID: 12586400
[TBL] [Abstract][Full Text] [Related]
15. Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase.
Kovacevic S; Weigel BJ; Tobin MB; Ingolia TD; Miller JR
J Bacteriol; 1989 Feb; 171(2):754-60. PubMed ID: 2644235
[TBL] [Abstract][Full Text] [Related]
16. C-terminus modification of Streptomyces clavuligerus deacetoxycephalosporin C synthase improves catalysis with an expanded substrate specificity.
Chin HS; Sim TS
Biochem Biophys Res Commun; 2002 Jul; 295(1):55-61. PubMed ID: 12083766
[TBL] [Abstract][Full Text] [Related]
17. Mutation of N304 to leucine in Streptomyces clavuligerus deacetoxycephalosporin C synthase creates an enzyme with increased penicillin analogue conversion.
Chin HS; Sim J; Sim TS
Biochem Biophys Res Commun; 2001 Sep; 287(2):507-13. PubMed ID: 11554757
[TBL] [Abstract][Full Text] [Related]
18. Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin G.
Gao Q; Piret JM; Adrio JL; Demain AL
J Ind Microbiol Biotechnol; 2003 Mar; 30(3):190-4. PubMed ID: 12715257
[TBL] [Abstract][Full Text] [Related]
19. The structural basis of cephalosporin formation in a mononuclear ferrous enzyme.
Valegård K; Terwisscha van Scheltinga AC; Dubus A; Ranghino G; Oster LM; Hajdu J; Andersson I
Nat Struct Mol Biol; 2004 Jan; 11(1):95-101. PubMed ID: 14718929
[TBL] [Abstract][Full Text] [Related]
20. The role of arginine residues in substrate binding and catalysis by deacetoxycephalosporin C synthase.
Lipscomb SJ; Lee HJ; Mukherji M; Baldwin JE; Schofield CJ; Lloyd MD
Eur J Biochem; 2002 Jun; 269(11):2735-9. PubMed ID: 12047382
[TBL] [Abstract][Full Text] [Related]
[Next] [New Search]