247 related articles for article (PubMed ID: 20816743)
1. A modified plasmid vector pCMV-3Tag-LIC for rapid, reliable, ligation-independent cloning of polymerase chain reaction products.
Du R; Li S; Zhang X
Anal Biochem; 2011 Jan; 408(2):357-9. PubMed ID: 20816743
[TBL] [Abstract][Full Text] [Related]
2. Preparation of long sticky ends for universal ligation-independent cloning: sequential T4 DNA polymerase treatments.
Tachibana A; Tohiguchi K; Ueno T; Setogawa Y; Harada A; Tanabe T
J Biosci Bioeng; 2009 Jun; 107(6):668-9. PubMed ID: 19447347
[TBL] [Abstract][Full Text] [Related]
3. A ligation-independent cloning method using nicking DNA endonuclease.
Yang J; Zhang Z; Zhang XA; Luo Q
Biotechniques; 2010 Nov; 49(5):817-21. PubMed ID: 21091446
[TBL] [Abstract][Full Text] [Related]
4. An efficient method for blunt-end ligation of PCR products.
Liu ZG; Schwartz LM
Biotechniques; 1992 Jan; 12(1):28, 30. PubMed ID: 1734919
[TBL] [Abstract][Full Text] [Related]
5. [A novel T-cloning vector system].
Köseoğlu O; Kocagöz T
Mikrobiyol Bul; 2004 Jul; 38(3):239-43. PubMed ID: 15490843
[TBL] [Abstract][Full Text] [Related]
6. An optimized recipe for cloning of the polymerase chain reaction-amplified DNA inserts into plasmid vectors.
Topcu Z
Acta Biochim Pol; 2000; 47(3):841-6. PubMed ID: 11310983
[TBL] [Abstract][Full Text] [Related]
7. A simple method to construct T-vectors using XcmI cassettes amplified by nonspecific PCR.
Jo C; Jo SA
Plasmid; 2001 Jan; 45(1):37-40. PubMed ID: 11319930
[TBL] [Abstract][Full Text] [Related]
8. A PCR-after-ligation method for cloning of multiple DNA inserts.
An Y; Wu W; Lv A
Anal Biochem; 2010 Jul; 402(2):203-5. PubMed ID: 20363207
[TBL] [Abstract][Full Text] [Related]
9. Minimal length requirement of the single-stranded tails for ligation-independent cloning (LIC) of PCR products.
Aslanidis C; de Jong PJ; Schmitz G
PCR Methods Appl; 1994 Dec; 4(3):172-7. PubMed ID: 7580902
[TBL] [Abstract][Full Text] [Related]
10. Ligation-independent cloning of PCR products (LIC-PCR).
Aslanidis C; de Jong PJ
Nucleic Acids Res; 1990 Oct; 18(20):6069-74. PubMed ID: 2235490
[TBL] [Abstract][Full Text] [Related]
11. Hierarchical ligation-independent assembly of PCR fragments.
Schmid-Burgk JL; Xie Z; Benenson Y
Methods Mol Biol; 2014; 1116():49-58. PubMed ID: 24395356
[TBL] [Abstract][Full Text] [Related]
12. A QuikChange-like method to realize efficient blunt-ended DNA directional cloning and site-directed mutagenesis simultaneously.
An Y; Lv A; Wu W
Biochem Biophys Res Commun; 2010 Jun; 397(2):136-9. PubMed ID: 20471367
[TBL] [Abstract][Full Text] [Related]
13. A vector for facile PCR product cloning and modification generating any desired 4-base 5' overhang: pRPM.
Cease KB; Lohff CJ
Biotechniques; 1993 Feb; 14(2):250-5. PubMed ID: 8431291
[TBL] [Abstract][Full Text] [Related]
14. Vector PCR.
Runnebaum IB; Syka P; Sukumar S
Biotechniques; 1991 Oct; 11(4):446-8, 450-2. PubMed ID: 1793574
[TBL] [Abstract][Full Text] [Related]
15. MEGAWHOP cloning: a method of creating random mutagenesis libraries via megaprimer PCR of whole plasmids.
Miyazaki K
Methods Enzymol; 2011; 498():399-406. PubMed ID: 21601687
[TBL] [Abstract][Full Text] [Related]
16. Creation of DNA overhangs by using modified DNA overhang cloning method.
Li W; Gao F
Appl Microbiol Biotechnol; 2007 Jun; 75(3):703-9. PubMed ID: 17308905
[TBL] [Abstract][Full Text] [Related]
17. Predictive mutagenesis of ligation-independent cloning (LIC) vectors for protein expression and site-specific chemical conjugation.
Vernet E; Sauer J; Andersen A; Jensen KJ; Voldborg B
Anal Biochem; 2011 Jul; 414(2):312-4. PubMed ID: 21414287
[TBL] [Abstract][Full Text] [Related]
18. Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors.
Haun RS; Serventi IM; Moss J
Biotechniques; 1992 Oct; 13(4):515-8. PubMed ID: 1362067
[No Abstract] [Full Text] [Related]
19. An efficient plasmid vector for expression cloning of large numbers of PCR fragments in Escherichia coli.
Reisinger C; Kern A; Fesko K; Schwab H
Appl Microbiol Biotechnol; 2007 Nov; 77(1):241-4. PubMed ID: 17786428
[TBL] [Abstract][Full Text] [Related]
20. Sticky PCR: A PCR-based protocol for targeted protein engineering.
Yamabhai M
Biotechnol J; 2009 Apr; 4(4):544-53. PubMed ID: 19370716
[TBL] [Abstract][Full Text] [Related]
[Next] [New Search]