BIOMARKERS

Molecular Biopsy of Human Tumors

- a resource for Precision Medicine *

219 related articles for article (PubMed ID: 20947674)

  • 1. Thermostabilization of an esterase by alignment-guided focussed directed evolution.
    Jochens H; Aerts D; Bornscheuer UT
    Protein Eng Des Sel; 2010 Dec; 23(12):903-9. PubMed ID: 20947674
    [TBL] [Abstract][Full Text] [Related]  

  • 2. Natural diversity to guide focused directed evolution.
    Jochens H; Bornscheuer UT
    Chembiochem; 2010 Sep; 11(13):1861-6. PubMed ID: 20680978
    [TBL] [Abstract][Full Text] [Related]  

  • 3. Knowledge-guided laboratory evolution of protein thermolability.
    Reetz MT; Soni P; Fernández L
    Biotechnol Bioeng; 2009 Apr; 102(6):1712-7. PubMed ID: 19072845
    [TBL] [Abstract][Full Text] [Related]  

  • 4. Increased enantioselectivity by engineering bottleneck mutants in an esterase from Pseudomonas fluorescens.
    Schliessmann A; Hidalgo A; Berenguer J; Bornscheuer UT
    Chembiochem; 2009 Dec; 10(18):2920-3. PubMed ID: 19847842
    [TBL] [Abstract][Full Text] [Related]  

  • 5. Thermostability enhancement of the Pseudomonas fluorescens esterase I by in vivo folding selection in Thermus thermophilus.
    Mate DM; Rivera NR; Sanchez-Freire E; Ayala JA; Berenguer J; Hidalgo A
    Biotechnol Bioeng; 2020 Jan; 117(1):30-38. PubMed ID: 31529702
    [TBL] [Abstract][Full Text] [Related]  

  • 6. Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations.
    Park S; Morley KL; Horsman GP; Holmquist M; Hult K; Kazlauskas RJ
    Chem Biol; 2005 Jan; 12(1):45-54. PubMed ID: 15664514
    [TBL] [Abstract][Full Text] [Related]  

  • 7. [Cloning, expression and characterization of a novel esterase from marine sediment microbial metagenomic library].
    Xu S; Hu Y; Yuan A; Zhu B
    Wei Sheng Wu Xue Bao; 2010 Jul; 50(7):891-6. PubMed ID: 20815235
    [TBL] [Abstract][Full Text] [Related]  

  • 8. Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase towards methyl 3bromo-2-methylpropanoate and ethyl 3phenylbutyrate.
    Horsman GP; Liu AM; Henke E; Bornscheuer UT; Kazlauskas RJ
    Chemistry; 2003 May; 9(9):1933-9. PubMed ID: 12740839
    [TBL] [Abstract][Full Text] [Related]  

  • 9. Directed evolution of an esterase from Pseudomonas fluorescens yields a mutant with excellent enantioselectivity and activity for the kinetic resolution of a chiral building block.
    Schmidt M; Hasenpusch D; Kähler M; Kirchner U; Wiggenhorn K; Langel W; Bornscheuer UT
    Chembiochem; 2006 May; 7(5):805-9. PubMed ID: 16575940
    [TBL] [Abstract][Full Text] [Related]  

  • 10. Directed evolution of an esterase from Pseudomonas fluorescens. Random mutagenesis by error-prone PCR or a mutator strain and identification of mutants showing enhanced enantioselectivity by a resorufin-based fluorescence assay.
    Henke E; Bornscheuer UT
    Biol Chem; 1999; 380(7-8):1029-33. PubMed ID: 10494857
    [TBL] [Abstract][Full Text] [Related]  

  • 11. Use of 'small but smart' libraries to enhance the enantioselectivity of an esterase from Bacillus stearothermophilus towards tetrahydrofuran-3-yl acetate.
    Nobili A; Gall MG; Pavlidis IV; Thompson ML; Schmidt M; Bornscheuer UT
    FEBS J; 2013 Jul; 280(13):3084-93. PubMed ID: 23331978
    [TBL] [Abstract][Full Text] [Related]  

  • 12. Biochemical characterization and mutational improvement of a thermophilic esterase from Sulfolobus solfataricus P2.
    Shang YS; Zhang XE; Wang XD; Guo YC; Zhang ZP; Zhou YF
    Biotechnol Lett; 2010 Aug; 32(8):1151-7. PubMed ID: 20386955
    [TBL] [Abstract][Full Text] [Related]  

  • 13. Directed evolution study of temperature adaptation in a psychrophilic enzyme.
    Miyazaki K; Wintrode PL; Grayling RA; Rubingh DN; Arnold FH
    J Mol Biol; 2000 Apr; 297(4):1015-26. PubMed ID: 10736234
    [TBL] [Abstract][Full Text] [Related]  

  • 14. Stability and activity improvement of cephalosporin esterase EstB from Burkholderia gladioli by directed evolution and structural interpretation of muteins.
    Valinger G; Hermann M; Wagner UG; Schwab H
    J Biotechnol; 2007 Mar; 129(1):98-108. PubMed ID: 17137667
    [TBL] [Abstract][Full Text] [Related]  

  • 15. Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability.
    Reetz MT; Carballeira JD; Vogel A
    Angew Chem Int Ed Engl; 2006 Nov; 45(46):7745-51. PubMed ID: 17075931
    [No Abstract]   [Full Text] [Related]  

  • 16. Screening and characterization of a novel esterase from a metagenomic library.
    Kim YJ; Choi GS; Kim SB; Yoon GS; Kim YS; Ryu YW
    Protein Expr Purif; 2006 Feb; 45(2):315-23. PubMed ID: 16061395
    [TBL] [Abstract][Full Text] [Related]  

  • 17. Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes.
    Reetz MT; Carballeira JD
    Nat Protoc; 2007; 2(4):891-903. PubMed ID: 17446890
    [TBL] [Abstract][Full Text] [Related]  

  • 18. A new cloning system using a mutant esterase containing MCS as an indicator for gene cloning.
    Cheong DE; Park SY; Shin HJ; Kim GJ
    J Microbiol Methods; 2009 Jun; 77(3):302-7. PubMed ID: 19341770
    [TBL] [Abstract][Full Text] [Related]  

  • 19. Improved thermostability of esterase from Aspergillus fumigatus by site-directed mutagenesis.
    Zhang S; Wu G; Feng S; Liu Z
    Enzyme Microb Technol; 2014 Oct; 64-65():11-6. PubMed ID: 25152411
    [TBL] [Abstract][Full Text] [Related]  

  • 20. Directed evolution of lipases and esterases.
    Schmidt M; Baumann M; Henke E; Konarzycka-Bessler M; Bornscheuer UT
    Methods Enzymol; 2004; 388():199-207. PubMed ID: 15289073
    [No Abstract]   [Full Text] [Related]  

    [Next]    [New Search]
    of 11.