These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
3. Circular polymerase extension cloning of complex gene libraries and pathways. Quan J; Tian J PLoS One; 2009 Jul; 4(7):e6441. PubMed ID: 19649325 [TBL] [Abstract][Full Text] [Related]
4. Combination of error-prone PCR (epPCR) and Circular Polymerase Extension Cloning (CPEC) for improving the coverage of random mutagenesis libraries. Ossa-Hernández N; Marins LF; Almeida DV Sci Rep; 2024 Jul; 14(1):15874. PubMed ID: 38982265 [TBL] [Abstract][Full Text] [Related]
5. Polishing the craft of genetic diversity creation in directed evolution. Tee KL; Wong TS Biotechnol Adv; 2013 Dec; 31(8):1707-21. PubMed ID: 24012599 [TBL] [Abstract][Full Text] [Related]
6. MEGAWHOP cloning: a method of creating random mutagenesis libraries via megaprimer PCR of whole plasmids. Miyazaki K Methods Enzymol; 2011; 498():399-406. PubMed ID: 21601687 [TBL] [Abstract][Full Text] [Related]
7. Generation of families of construct variants using golden gate shuffling. Engler C; Marillonnet S Methods Mol Biol; 2011; 729():167-81. PubMed ID: 21365490 [TBL] [Abstract][Full Text] [Related]
8. Standardized cloning vectors for protein production and generation of large gene libraries in Escherichia coli. Rohweder B; Semmelmann F; Endres C; Sterner R Biotechniques; 2018 Jan; 64(1):24-26. PubMed ID: 29384074 [TBL] [Abstract][Full Text] [Related]
9. RF cloning: a restriction-free method for inserting target genes into plasmids. van den Ent F; Löwe J J Biochem Biophys Methods; 2006 Apr; 67(1):67-74. PubMed ID: 16480772 [TBL] [Abstract][Full Text] [Related]
10. Restriction-ligation-free (RLF) cloning: a high-throughput cloning method by in vivo homologous recombination of PCR products. Wang Y; Liu Y; Chen J; Tang MJ; Zhang SL; Wei LN; Li CH; Wei DB Genet Mol Res; 2015 Oct; 14(4):12306-15. PubMed ID: 26505379 [TBL] [Abstract][Full Text] [Related]
11. An optimized recipe for cloning of the polymerase chain reaction-amplified DNA inserts into plasmid vectors. Topcu Z Acta Biochim Pol; 2000; 47(3):841-6. PubMed ID: 11310983 [TBL] [Abstract][Full Text] [Related]
12. Ligation-independent cloning of PCR products (LIC-PCR). Aslanidis C; de Jong PJ Nucleic Acids Res; 1990 Oct; 18(20):6069-74. PubMed ID: 2235490 [TBL] [Abstract][Full Text] [Related]
13. Optimised cDNA size selection and cloning procedure for the construction of representative plasmid cDNA libraries. Kieffer BL Gene; 1991 Dec; 109(1):115-9. PubMed ID: 1756970 [TBL] [Abstract][Full Text] [Related]
14. Cloning in Plasmid Vectors: Blunt-End Cloning. Green MR; Sambrook J Cold Spring Harb Protoc; 2020 Nov; 2020(11):. PubMed ID: 33139501 [TBL] [Abstract][Full Text] [Related]
15. The plasmid vectors, pBS2ndd and pBS3ndd, for versatile cloning with low background in Escherichia coli. Rotchanapreeda T; Ngonsawan W; Klomtun M; Somana J World J Microbiol Biotechnol; 2018 Jun; 34(6):85. PubMed ID: 29876748 [TBL] [Abstract][Full Text] [Related]
16. Optimal cloning of PCR fragments by homologous recombination in Escherichia coli. Jacobus AP; Gross J PLoS One; 2015; 10(3):e0119221. PubMed ID: 25774528 [TBL] [Abstract][Full Text] [Related]
17. Linear plasmid vector for cloning of repetitive or unstable sequences in Escherichia coli. Godiska R; Mead D; Dhodda V; Wu C; Hochstein R; Karsi A; Usdin K; Entezam A; Ravin N Nucleic Acids Res; 2010 Apr; 38(6):e88. PubMed ID: 20040575 [TBL] [Abstract][Full Text] [Related]
18. Rapid restriction enzyme-free cloning of PCR products: a high-throughput method applicable for library construction. Chaudhary VK; Shrivastava N; Verma V; Das S; Kaur C; Grover P; Gupta A PLoS One; 2014; 9(10):e111538. PubMed ID: 25360695 [TBL] [Abstract][Full Text] [Related]
19. MegaGate: A toxin-less gateway molecular cloning tool. Kramme C; Plesa AM; Wang HH; Wolf B; Smela MP; Guo X; Kohman RE; Chatterjee P; Church GM STAR Protoc; 2021 Dec; 2(4):100907. PubMed ID: 34746865 [TBL] [Abstract][Full Text] [Related]
20. pKILLIN: a versatile positive-selection cloning vector based on the toxicity of Killin in Escherichia coli. Ma Z; Luo D; Huang A; Xu Y; Wang Y; Wei Y; Liang P Gene; 2014 Jul; 544(2):228-35. PubMed ID: 24768186 [TBL] [Abstract][Full Text] [Related] [Next] [New Search]