74 related articles for article (PubMed ID: 21815085)
1. Scarless chromosomal gene knockout methods.
Sung BH; Lee JH; Kim SC
Methods Mol Biol; 2011; 765():43-54. PubMed ID: 21815085
[TBL] [Abstract][Full Text] [Related]
2. Rapid and efficient construction of markerless deletions in the Escherichia coli genome.
Yu BJ; Kang KH; Lee JH; Sung BH; Kim MS; Kim SC
Nucleic Acids Res; 2008 Aug; 36(14):e84. PubMed ID: 18567910
[TBL] [Abstract][Full Text] [Related]
3. Targeted chromosomal gene knockout using PCR fragments.
Murphy KC
Methods Mol Biol; 2011; 765():27-42. PubMed ID: 21815084
[TBL] [Abstract][Full Text] [Related]
4. Rapid one-step inactivation of single or multiple genes in Escherichia coli.
Song CW; Lee SY
Biotechnol J; 2013 Jul; 8(7):776-84. PubMed ID: 23653342
[TBL] [Abstract][Full Text] [Related]
5. Scarless engineering of the Escherichia coli genome.
Fehér T; Karcagi I; Gyorfy Z; Umenhoffer K; Csörgo B; Pósfai G
Methods Mol Biol; 2008; 416():251-9. PubMed ID: 18392972
[TBL] [Abstract][Full Text] [Related]
6. [Gene knockout and knockin on the Escherichia coli lac operon loci using pBR322-red system].
Chen W; Yu M; Li SH; Wang MG; Zhou JG
Sheng Wu Gong Cheng Xue Bao; 2005 Mar; 21(2):192-7. PubMed ID: 16013474
[TBL] [Abstract][Full Text] [Related]
7. Marker-free chromosomal expression of foreign and native genes in Escherichia coli.
Chiang CJ; Chen PT; Chen SY; Chao YP
Methods Mol Biol; 2011; 765():113-23. PubMed ID: 21815090
[TBL] [Abstract][Full Text] [Related]
8. Introduction of conditional lethal amber mutations in Escherichia coli.
Herring CD
Methods Mol Biol; 2008; 416():323-34. PubMed ID: 18392977
[TBL] [Abstract][Full Text] [Related]
9. Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome.
Kato J; Hashimoto M
Methods Mol Biol; 2008; 416():279-93. PubMed ID: 18392974
[TBL] [Abstract][Full Text] [Related]
10. An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda Red.
Fukiya S; Mizoguchi H; Mori H
FEMS Microbiol Lett; 2004 May; 234(2):325-31. PubMed ID: 15135540
[TBL] [Abstract][Full Text] [Related]
11. Replicon-free and markerless methods for genomic insertion of DNAs in phage attachment sites and controlled expression of chromosomal genes in Escherichia coli.
Chiang CJ; Chen PT; Chao YP
Biotechnol Bioeng; 2008 Dec; 101(5):985-95. PubMed ID: 18553504
[TBL] [Abstract][Full Text] [Related]
12. Construction of targeted mycobacterial mutants by homologous recombination.
Kendall SL; Frita R
Methods Mol Biol; 2009; 465():297-310. PubMed ID: 20560068
[TBL] [Abstract][Full Text] [Related]
13. High-efficiency scarless genetic modification in Escherichia coli by using lambda red recombination and I-SceI cleavage.
Yang J; Sun B; Huang H; Jiang Y; Diao L; Chen B; Xu C; Wang X; Liu J; Jiang W; Yang S
Appl Environ Microbiol; 2014 Jul; 80(13):3826-34. PubMed ID: 24747889
[TBL] [Abstract][Full Text] [Related]
14. [Tuning of expression level of the genes of interest located in the bacterial chromosome].
Katashkina ZhI; Skorokhodova AIu; Zimenkov DV; Gulevich AIu; Minaeva NI; Doroshenko VG; Biriukova IV; Mashko SV
Mol Biol (Mosk); 2005; 39(5):823-31. PubMed ID: 16240716
[TBL] [Abstract][Full Text] [Related]
15. One step construction of Agrobacterium-Recombination-ready-plasmids (OSCAR), an efficient and robust tool for ATMT based gene deletion construction in fungi.
Paz Z; García-Pedrajas MD; Andrews DL; Klosterman SJ; Baeza-Montañez L; Gold SE
Fungal Genet Biol; 2011 Jul; 48(7):677-84. PubMed ID: 21362493
[TBL] [Abstract][Full Text] [Related]
16. [Markerless DNA deletion based on Red recombination and in vivo I-Sec I endonuclease cleavage in Escherichia coli chromosome].
Zhu M; Yu J; Zhou C; Fang H
Sheng Wu Gong Cheng Xue Bao; 2016 Jan; 32(1):114-26. PubMed ID: 27363204
[TBL] [Abstract][Full Text] [Related]
17. Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress.
Jensen SI; Lennen RM; Herrgård MJ; Nielsen AT
Sci Rep; 2015 Dec; 5():17874. PubMed ID: 26643270
[TBL] [Abstract][Full Text] [Related]
18. Xer-cise in Helicobacter pylori: one-step transformation for the construction of markerless gene deletions.
Debowski AW; Gauntlett JC; Li H; Liao T; Sehnal M; Nilsson HO; Marshall BJ; Benghezal M
Helicobacter; 2012 Dec; 17(6):435-43. PubMed ID: 23066820
[TBL] [Abstract][Full Text] [Related]
19. Multiple large segment deletion method for Corynebacterium glutamicum.
Suzuki N; Nonaka H; Tsuge Y; Okayama S; Inui M; Yukawa H
Appl Microbiol Biotechnol; 2005 Nov; 69(2):151-61. PubMed ID: 15843930
[TBL] [Abstract][Full Text] [Related]
20. New tool for metabolic pathway engineering in Escherichia coli: one-step method to modulate expression of chromosomal genes.
Meynial-Salles I; Cervin MA; Soucaille P
Appl Environ Microbiol; 2005 Apr; 71(4):2140-4. PubMed ID: 15812048
[TBL] [Abstract][Full Text] [Related]
[Next] [New Search]