These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
3. Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus. Skowron PM; Vitkute J; Ramanauskaite D; Mitkaite G; Jezewska-Frackowiak J; Zebrowska J; Zylicz-Stachula A; Lubys A BMC Mol Biol; 2013 Aug; 14():17. PubMed ID: 23919831 [TBL] [Abstract][Full Text] [Related]
4. A new Thermus sp. class-IIS enzyme sub-family: isolation of a 'twin' endonuclease TspDTI with a novel specificity 5'-ATGAA(N(11/9))-3', related to TspGWI, TaqII and Tth111II. Skowron PM; Majewski J; Zylicz-Stachula A; Rutkowska SM; Jaworowska I; Harasimowicz-Słowińska RI Nucleic Acids Res; 2003 Jul; 31(14):e74. PubMed ID: 12853651 [TBL] [Abstract][Full Text] [Related]
6. The third restriction-modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities. Skowron PM; Anton BP; Czajkowska E; Zebrowska J; Sulecka E; Krefft D; Jezewska-Frackowiak J; Zolnierkiewicz O; Witkowska M; Morgan RD; Wilson GG; Fomenkov A; Roberts RJ; Zylicz-Stachula A Nucleic Acids Res; 2017 Sep; 45(15):9005-9018. PubMed ID: 28911108 [TBL] [Abstract][Full Text] [Related]
7. Two-stage gene assembly/cloning of a member of the TspDTI subfamily of bifunctional restriction endonucleases, TthHB27I. Krefft D; Zylicz-Stachula A; Mulkiewicz E; Papkov A; Jezewska-Frackowiak J; Skowron PM J Biotechnol; 2015 Jan; 194():67-80. PubMed ID: 25486633 [TBL] [Abstract][Full Text] [Related]
8. Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family. Zylicz-Stachula A; Bujnicki JM; Skowron PM BMC Mol Biol; 2009 May; 10():52. PubMed ID: 19480701 [TBL] [Abstract][Full Text] [Related]
10. A new genomic tool, ultra-frequently cleaving TaqII/sinefungin endonuclease with a combined 2.9-bp recognition site, applied to the construction of horse DNA libraries. Zylicz-Stachula A; Zolnierkiewicz O; Jasiecki J; Skowron PM BMC Genomics; 2013 Jun; 14():370. PubMed ID: 23724933 [TBL] [Abstract][Full Text] [Related]
11. Novel parameter describing restriction endonucleases: Secondary-Cognate-Specificity and chemical stimulation of TsoI leading to substrate specificity change. Zebrowska J; Jezewska-Frackowiak J; Wieczerzak E; Kasprzykowski F; Zylicz-Stachula A; Skowron PM Appl Microbiol Biotechnol; 2019 Apr; 103(8):3439-3451. PubMed ID: 30879089 [TBL] [Abstract][Full Text] [Related]
12. Engineering TaqII bifunctional endonuclease DNA recognition fidelity: the effect of a single amino acid substitution within the methyltransferase catalytic site. Zylicz-Stachula A; Zebrowska J; Czajkowska E; Wrese W; Sulecka E; Skowron PM Mol Biol Rep; 2016 Apr; 43(4):269-82. PubMed ID: 26886214 [TBL] [Abstract][Full Text] [Related]
15. A putative Type IIS restriction endonuclease GeoICI from Geobacillus sp.--A robust, thermostable alternative to mezophilic prototype BbvI. Zebrowska J; Zolnierkiewicz O; Skowron MA; Zylicz-Stachula A; Jezewska-Frackowiak J; Skowron PM J Biosci; 2016 Mar; 41(1):27-38. PubMed ID: 26949085 [TBL] [Abstract][Full Text] [Related]
16. A second type II restriction endonuclease from Thermus aquaticus with an unusual sequence specificity. Barker D; Hoff M; Oliphant A; White R Nucleic Acids Res; 1984 Jul; 12(14):5567-81. PubMed ID: 6087291 [TBL] [Abstract][Full Text] [Related]
17. Randomized DNA libraries construction tool: a new 3-bp 'frequent cutter' TthHB27I/sinefungin endonuclease with chemically-induced specificity. Krefft D; Papkov A; Prusinowski M; Zylicz-Stachula A; Skowron PM BMC Genomics; 2018 May; 19(1):361. PubMed ID: 29751745 [TBL] [Abstract][Full Text] [Related]
18. Novel subtype of type IIs restriction enzymes. BfiI endonuclease exhibits similarities to the EDTA-resistant nuclease Nuc of Salmonella typhimurium. Sapranauskas R; Sasnauskas G; Lagunavicius A; Vilkaitis G; Lubys A; Siksnys V J Biol Chem; 2000 Oct; 275(40):30878-85. PubMed ID: 10880511 [TBL] [Abstract][Full Text] [Related]
19. Creation of a type IIS restriction endonuclease with a long recognition sequence. Lippow SM; Aha PM; Parker MH; Blake WJ; Baynes BM; Lipovsek D Nucleic Acids Res; 2009 May; 37(9):3061-73. PubMed ID: 19304757 [TBL] [Abstract][Full Text] [Related]
20. Two new thermostable type II restriction endonucleases from Thermus aquaticus: TatI and TauI, which recognize the novel nucleotide sequences 5'-W (downward arrow)GTACW-3' and 5'-GCSG (downward arrow)C-3' respectively. Vitkute J; Maneliene Z; Janulaitis A FEMS Microbiol Lett; 2001 Nov; 204(2):253-7. PubMed ID: 11731131 [TBL] [Abstract][Full Text] [Related] [Next] [New Search]