These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


BIOMARKERS

Molecular Biopsy of Human Tumors

- a resource for Precision Medicine *

186 related articles for article (PubMed ID: 22694812)

  • 21. High-level secretion of native recombinant human calreticulin in yeast.
    Čiplys E; Žitkus E; Gold LI; Daubriac J; Pavlides SC; Højrup P; Houen G; Wang WA; Michalak M; Slibinskas R
    Microb Cell Fact; 2015 Oct; 14():165. PubMed ID: 26471510
    [TBL] [Abstract][Full Text] [Related]  

  • 22. High-throughput plasmid construction using homologous recombination in yeast: its mechanisms and application to protein production for X-ray crystallography.
    Mizutani K
    Biosci Biotechnol Biochem; 2015; 79(1):1-10. PubMed ID: 25603812
    [TBL] [Abstract][Full Text] [Related]  

  • 23. Screening for high-yielding Pichia pastoris clones: the production of G protein-coupled receptors as a case study.
    Singh S; Gras A; Fiez-Vandal C; Martinez M; Wagner R; Byrne B
    Methods Mol Biol; 2012; 866():65-73. PubMed ID: 22454115
    [TBL] [Abstract][Full Text] [Related]  

  • 24. DCyFIR: a high-throughput CRISPR platform for multiplexed G protein-coupled receptor profiling and ligand discovery.
    Kapolka NJ; Taghon GJ; Rowe JB; Morgan WM; Enten JF; Lambert NA; Isom DG
    Proc Natl Acad Sci U S A; 2020 Jun; 117(23):13117-13126. PubMed ID: 32434907
    [TBL] [Abstract][Full Text] [Related]  

  • 25. Screening for high-yielding Saccharomyces cerevisiae clones: using a green fluorescent protein fusion strategy in the production of membrane proteins.
    Drew D; Kim H
    Methods Mol Biol; 2012; 866():75-86. PubMed ID: 22454116
    [TBL] [Abstract][Full Text] [Related]  

  • 26. Dot-blot immunodetection as a versatile and high-throughput assay to evaluate recombinant GPCRs produced in the yeast Pichia pastoris.
    Zeder-Lutz G; Cherouati N; Reinhart C; Pattus F; Wagner R
    Protein Expr Purif; 2006 Nov; 50(1):118-27. PubMed ID: 16843005
    [TBL] [Abstract][Full Text] [Related]  

  • 27. Production of human caseinomacropeptide in recombinant Saccharomyces cerevisiae and Pichia pastoris.
    Kim YJ; Oh YK; Kang W; Lee EY; Park S
    J Ind Microbiol Biotechnol; 2005 Sep; 32(9):402-8. PubMed ID: 16078069
    [TBL] [Abstract][Full Text] [Related]  

  • 28. Expression of GPCRs in Pichia pastoris for structural studies.
    Krettler C; Reinhart C; Bevans CG
    Methods Enzymol; 2013; 520():1-29. PubMed ID: 23332693
    [TBL] [Abstract][Full Text] [Related]  

  • 29. Expression and Purification of Human Membrane Progestin Receptor α (mPRα).
    Hossain MB; Oshima T; Hirose S; Wang J; Tokumoto T
    PLoS One; 2015; 10(9):e0138739. PubMed ID: 26398701
    [TBL] [Abstract][Full Text] [Related]  

  • 30. Improved identification of agonist-mediated Gα(i)-specific human G-protein-coupled receptor signaling in yeast cells by flow cytometry.
    Ishii J; Moriguchi M; Hara KY; Shibasaki S; Fukuda H; Kondo A
    Anal Biochem; 2012 Jul; 426(2):129-33. PubMed ID: 22504120
    [TBL] [Abstract][Full Text] [Related]  

  • 31. Recovery of the histamine H
    Watanabe A; Nakajima A; Shiroishi M
    Sci Rep; 2023 Sep; 13(1):16127. PubMed ID: 37752220
    [TBL] [Abstract][Full Text] [Related]  

  • 32. Yeast-based fluorescence reporter assay of G protein-coupled receptor signalling for flow cytometric screening: FAR1-disruption recovers loss of episomal plasmid caused by signalling in yeast.
    Ishii J; Tanaka T; Matsumura S; Tatematsu K; Kuroda S; Ogino C; Fukuda H; Kondo A
    J Biochem; 2008 May; 143(5):667-74. PubMed ID: 18281298
    [TBL] [Abstract][Full Text] [Related]  

  • 33. Universal platform for the generation of thermostabilized GPCRs that crystallize in LCP.
    Schöppe J; Ehrenmann J; Waltenspühl Y; Plückthun A
    Nat Protoc; 2022 Mar; 17(3):698-726. PubMed ID: 35140409
    [TBL] [Abstract][Full Text] [Related]  

  • 34. Characterization of 16 human G protein-coupled receptors expressed in baculovirus-infected insect cells.
    Akermoun M; Koglin M; Zvalova-Iooss D; Folschweiller N; Dowell SJ; Gearing KL
    Protein Expr Purif; 2005 Nov; 44(1):65-74. PubMed ID: 15951199
    [TBL] [Abstract][Full Text] [Related]  

  • 35. G protein-coupled receptors expressed and studied in yeast. The adenosine receptor as a prime example.
    Wang X; van Westen GJP; Heitman LH; IJzerman AP
    Biochem Pharmacol; 2021 May; 187():114370. PubMed ID: 33338473
    [TBL] [Abstract][Full Text] [Related]  

  • 36. Establishing a versatile fermentation and purification procedure for human proteins expressed in the yeasts Saccharomyces cerevisiae and Pichia pastoris for structural genomics.
    Prinz B; Schultchen J; Rydzewski R; Holz C; Boettner M; Stahl U; Lang C
    J Struct Funct Genomics; 2004; 5(1-2):29-44. PubMed ID: 15263841
    [TBL] [Abstract][Full Text] [Related]  

  • 37. High yield purification of full-length functional hERG K+ channels produced in Saccharomyces cerevisiae.
    Molbaek K; Scharff-Poulsen P; Helix-Nielsen C; Klaerke DA; Pedersen PA
    Microb Cell Fact; 2015 Feb; 14():15. PubMed ID: 25656388
    [TBL] [Abstract][Full Text] [Related]  

  • 38. Heterologous GPCR expression: a bottleneck to obtaining crystal structures.
    McCusker EC; Bane SE; O'Malley MA; Robinson AS
    Biotechnol Prog; 2007; 23(3):540-7. PubMed ID: 17397185
    [TBL] [Abstract][Full Text] [Related]  

  • 39. Production, purification, and characterization of human scFv antibodies expressed in Saccharomyces cerevisiae, Pichia pastoris, and Escherichia coli.
    Miller KD; Weaver-Feldhaus J; Gray SA; Siegel RW; Feldhaus MJ
    Protein Expr Purif; 2005 Aug; 42(2):255-67. PubMed ID: 15946857
    [TBL] [Abstract][Full Text] [Related]  

  • 40. Tuning Vector Stability and Integration Frequency Elevates Functional GPCR Production and Homogeneity in Saccharomyces cerevisiae.
    Yoo JI; O'Malley MA
    ACS Synth Biol; 2018 Jul; 7(7):1763-1772. PubMed ID: 29871481
    [TBL] [Abstract][Full Text] [Related]  

    [Previous]   [Next]    [New Search]
    of 10.