BIOMARKERS

Molecular Biopsy of Human Tumors

- a resource for Precision Medicine *

153 related articles for article (PubMed ID: 22923414)

  • 1. Iterative combinatorial mutagenesis as an effective strategy for generation of deacetoxycephalosporin C synthase with improved activity toward penicillin G.
    Ji J; Fan K; Tian X; Zhang X; Zhang Y; Yang K
    Appl Environ Microbiol; 2012 Nov; 78(21):7809-12. PubMed ID: 22923414
    [TBL] [Abstract][Full Text] [Related]  

  • 2. Directed evolution of Streptomyces clavuligerus deacetoxycephalosporin C synthase for enhancement of penicillin G expansion.
    Wei CL; Yang YB; Deng CH; Liu WC; Hsu JS; Lin YC; Liaw SH; Tsai YC
    Appl Environ Microbiol; 2005 Dec; 71(12):8873-80. PubMed ID: 16332884
    [TBL] [Abstract][Full Text] [Related]  

  • 3. New strategy of site-directed mutagenesis identifies new sites to improve Streptomyces clavuligerus deacetoxycephalosporin C synthase activity toward penicillin G.
    Ji J; Tian X; Fan K; Yang K
    Appl Microbiol Biotechnol; 2012 Mar; 93(6):2395-401. PubMed ID: 21909691
    [TBL] [Abstract][Full Text] [Related]  

  • 4. The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S. clavuligerus.
    Lee HJ; Lloyd MD; Harlos K; Schofield CJ
    Biochem Biophys Res Commun; 2000 Jan; 267(1):445-8. PubMed ID: 10623639
    [TBL] [Abstract][Full Text] [Related]  

  • 5. Relevant double mutations in bioengineered Streptomyces clavuligerus deacetoxycephalosporin C synthase result in higher binding specificities which improve penicillin bioconversion.
    Goo KS; Chua CS; Sim TS
    Appl Environ Microbiol; 2008 Feb; 74(4):1167-75. PubMed ID: 18083859
    [TBL] [Abstract][Full Text] [Related]  

  • 6. Mutation of N304 to leucine in Streptomyces clavuligerus deacetoxycephalosporin C synthase creates an enzyme with increased penicillin analogue conversion.
    Chin HS; Sim J; Sim TS
    Biochem Biophys Res Commun; 2001 Sep; 287(2):507-13. PubMed ID: 11554757
    [TBL] [Abstract][Full Text] [Related]  

  • 7. A complete library of amino acid alterations at R306 in Streptomyces clavuligerus deacetoxycephalosporin C synthase demonstrates its structural role in the ring-expansion activity.
    Sim Goo K; Song Chua C; Sim TS
    Proteins; 2008 Feb; 70(3):739-47. PubMed ID: 17729280
    [TBL] [Abstract][Full Text] [Related]  

  • 8. C-terminus modification of Streptomyces clavuligerus deacetoxycephalosporin C synthase improves catalysis with an expanded substrate specificity.
    Chin HS; Sim TS
    Biochem Biophys Res Commun; 2002 Jul; 295(1):55-61. PubMed ID: 12083766
    [TBL] [Abstract][Full Text] [Related]  

  • 9. Mutational evidence supporting the involvement of tripartite residues His183, Asp185, and His243 in Streptomyces clavuligerus deacetoxycephalosporin C synthase for catalysis.
    Sim J; Sim TS
    Biosci Biotechnol Biochem; 2000 Apr; 64(4):828-32. PubMed ID: 10830499
    [TBL] [Abstract][Full Text] [Related]  

  • 10. A complete library of amino acid alterations at N304 in Streptomyces clavuligerus deacetoxycephalosporin C synthase elucidates the basis for enhanced penicillin analogue conversion.
    Chin HS; Goo KS; Sim TS
    Appl Environ Microbiol; 2004 Jan; 70(1):607-9. PubMed ID: 14711695
    [TBL] [Abstract][Full Text] [Related]  

  • 11. Family shuffling of expandase genes to enhance substrate specificity for penicillin G.
    Hsu JS; Yang YB; Deng CH; Wei CL; Liaw SH; Tsai YC
    Appl Environ Microbiol; 2004 Oct; 70(10):6257-63. PubMed ID: 15466573
    [TBL] [Abstract][Full Text] [Related]  

  • 12. Directed evolution and rational approaches to improving Streptomyces clavuligerus deacetoxycephalosporin C synthase for cephalosporin production.
    Goo KS; Chua CS; Sim TS
    J Ind Microbiol Biotechnol; 2009 May; 36(5):619-33. PubMed ID: 19277744
    [TBL] [Abstract][Full Text] [Related]  

  • 13. Engineering Streptomyces clavuligerus deacetoxycephalosporin C synthase for optimal ring expansion activity toward penicillin G.
    Wei CL; Yang YB; Wang WC; Liu WC; Hsu JS; Tsai YC
    Appl Environ Microbiol; 2003 Apr; 69(4):2306-12. PubMed ID: 12676714
    [TBL] [Abstract][Full Text] [Related]  

  • 14. Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin G.
    Gao Q; Piret JM; Adrio JL; Demain AL
    J Ind Microbiol Biotechnol; 2003 Mar; 30(3):190-4. PubMed ID: 12715257
    [TBL] [Abstract][Full Text] [Related]  

  • 15. Probing the penicillin sidechain selectivity of recombinant deacetoxycephalosporin C synthase.
    Dubus A; Lloyd MD; Lee HJ; Schofield CJ; Baldwin JE; Frere JM
    Cell Mol Life Sci; 2001 May; 58(5-6):835-43. PubMed ID: 11437242
    [TBL] [Abstract][Full Text] [Related]  

  • 16. Elucidation of conditions allowing conversion of penicillin G and other penicillins to deacetoxycephalosporins by resting cells and extracts of Streptomyces clavuligerus NP1.
    Cho H; Adrio JL; Luengo JM; Wolfe S; Ocran S; Hintermann G; Piret JM; Demain AL
    Proc Natl Acad Sci U S A; 1998 Sep; 95(20):11544-8. PubMed ID: 9751702
    [TBL] [Abstract][Full Text] [Related]  

  • 17. Active site mutations of recombinant deacetoxycephalosporin C synthase.
    Lee HJ; Schofield CJ; Lloyd MD
    Biochem Biophys Res Commun; 2002 Mar; 292(1):66-70. PubMed ID: 11890672
    [TBL] [Abstract][Full Text] [Related]  

  • 18. Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase.
    Lloyd MD; Lipscomb SJ; Hewitson KS; Hensgens CM; Baldwin JE; Schofield CJ
    J Biol Chem; 2004 Apr; 279(15):15420-6. PubMed ID: 14734549
    [TBL] [Abstract][Full Text] [Related]  

  • 19. Whole-Cell Biotransformation of Penicillin G by a Three-Enzyme Co-expression System with Engineered Deacetoxycephalosporin C Synthase.
    Song S; Jiang Y; Chen R; Su W; Liang W; Yang D; Li J; Zhang W; Gao S; Yuan B; Qu G; Sun Z
    Chembiochem; 2022 Jun; 23(11):e202200179. PubMed ID: 35384232
    [TBL] [Abstract][Full Text] [Related]  

  • 20. Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS).
    Lee HJ; Lloyd MD; Harlos K; Clifton IJ; Baldwin JE; Schofield CJ
    J Mol Biol; 2001 May; 308(5):937-48. PubMed ID: 11352583
    [TBL] [Abstract][Full Text] [Related]  

    [Next]    [New Search]
    of 8.