328 related articles for article (PubMed ID: 23504429)
1. Construction and analysis of randomized protein-encoding libraries using error-prone PCR.
Hanson-Manful P; Patrick WM
Methods Mol Biol; 2013; 996():251-67. PubMed ID: 23504429
[TBL] [Abstract][Full Text] [Related]
2. Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations.
Zhao J; Kardashliev T; Joëlle Ruff A; Bocola M; Schwaneberg U
Biotechnol Bioeng; 2014 Dec; 111(12):2380-9. PubMed ID: 24904008
[TBL] [Abstract][Full Text] [Related]
3. Error-prone PCR and effective generation of gene variant libraries for directed evolution.
Copp JN; Hanson-Manful P; Ackerley DF; Patrick WM
Methods Mol Biol; 2014; 1179():3-22. PubMed ID: 25055767
[TBL] [Abstract][Full Text] [Related]
4. Polishing the craft of genetic diversity creation in directed evolution.
Tee KL; Wong TS
Biotechnol Adv; 2013 Dec; 31(8):1707-21. PubMed ID: 24012599
[TBL] [Abstract][Full Text] [Related]
5. Development and Use of a Novel Random Mutagenesis Method: In Situ Error-Prone PCR (is-epPCR).
Shao W; Ma K; Le Y; Wang H; Sha C
Methods Mol Biol; 2017; 1498():497-506. PubMed ID: 27709598
[TBL] [Abstract][Full Text] [Related]
6. Amino acid substitutions in random mutagenesis libraries: lessons from analyzing 3000 mutations.
Zhao J; Frauenkron-Machedjou VJ; Kardashliev T; Ruff AJ; Zhu L; Bocola M; Schwaneberg U
Appl Microbiol Biotechnol; 2017 Apr; 101(8):3177-3187. PubMed ID: 28050632
[TBL] [Abstract][Full Text] [Related]
7. Combinatorial engineering to enhance amylosucrase performance: construction, selection, and screening of variant libraries for increased activity.
van der Veen BA; Potocki-Véronèse G; Albenne C; Joucla G; Monsan P; Remaud-Simeon M
FEBS Lett; 2004 Feb; 560(1-3):91-7. PubMed ID: 14988004
[TBL] [Abstract][Full Text] [Related]
8. SeSaM-Tv-II generates a protein sequence space that is unobtainable by epPCR.
Mundhada H; Marienhagen J; Scacioc A; Schenk A; Roccatano D; Schwaneberg U
Chembiochem; 2011 Jul; 12(10):1595-601. PubMed ID: 21671328
[TBL] [Abstract][Full Text] [Related]
9. User-friendly algorithms for estimating completeness and diversity in randomized protein-encoding libraries.
Patrick WM; Firth AE; Blackburn JM
Protein Eng; 2003 Jun; 16(6):451-7. PubMed ID: 12874379
[TBL] [Abstract][Full Text] [Related]
10. Construction of diverse adeno-associated viral libraries for directed evolution of enhanced gene delivery vehicles.
Koerber JT; Maheshri N; Kaspar BK; Schaffer DV
Nat Protoc; 2006; 1(2):701-6. PubMed ID: 17406299
[TBL] [Abstract][Full Text] [Related]
11. Casting epPCR (cepPCR): A simple random mutagenesis method to generate high quality mutant libraries.
Yang J; Ruff AJ; Arlt M; Schwaneberg U
Biotechnol Bioeng; 2017 Sep; 114(9):1921-1927. PubMed ID: 28464223
[TBL] [Abstract][Full Text] [Related]
12. Random mutagenesis by error-prone PCR.
McCullum EO; Williams BA; Zhang J; Chaput JC
Methods Mol Biol; 2010; 634():103-9. PubMed ID: 20676978
[TBL] [Abstract][Full Text] [Related]
13. GLUE-IT and PEDEL-AA: new programmes for analyzing protein diversity in randomized libraries.
Firth AE; Patrick WM
Nucleic Acids Res; 2008 Jul; 36(Web Server issue):W281-5. PubMed ID: 18442989
[TBL] [Abstract][Full Text] [Related]
14. Fast and Flexible Synthesis of Combinatorial Libraries for Directed Evolution.
Sadler JC; Green L; Swainston N; Kell DB; Currin A
Methods Enzymol; 2018; 608():59-79. PubMed ID: 30173773
[TBL] [Abstract][Full Text] [Related]
15. The "Bringer" strategy: a very fast and highly efficient method for construction of mutant libraries by error-prone polymerase chain reaction of ring-closed plasmids.
Bichet A; Bureik M; Lenz N; Bernhardt R
Appl Biochem Biotechnol; 2004 May; 117(2):115-22. PubMed ID: 15159555
[TBL] [Abstract][Full Text] [Related]
16. Mutant library construction in directed molecular evolution: casting a wider net.
Wang TW; Zhu H; Ma XY; Zhang T; Ma YS; Wei DZ
Mol Biotechnol; 2006 Sep; 34(1):55-68. PubMed ID: 16943572
[TBL] [Abstract][Full Text] [Related]
17. Probabilistic methods in directed evolution: library size, mutation rate, and diversity.
Nov Y
Methods Mol Biol; 2014; 1179():261-78. PubMed ID: 25055784
[TBL] [Abstract][Full Text] [Related]
18. Strategies and computational tools for improving randomized protein libraries.
Patrick WM; Firth AE
Biomol Eng; 2005 Oct; 22(4):105-12. PubMed ID: 16095966
[TBL] [Abstract][Full Text] [Related]
19. Synthetic gene libraries: in search of the optimal diversity.
Ostermeier M
Trends Biotechnol; 2003 Jun; 21(6):244-7. PubMed ID: 12788541
[TBL] [Abstract][Full Text] [Related]
20. One-step combined focused epPCR and saturation mutagenesis for thermostability evolution of a new cold-active xylanase.
Acevedo JP; Reetz MT; Asenjo JA; Parra LP
Enzyme Microb Technol; 2017 May; 100():60-70. PubMed ID: 28284313
[TBL] [Abstract][Full Text] [Related]
[Next] [New Search]