BIOMARKERS

Molecular Biopsy of Human Tumors

- a resource for Precision Medicine *

339 related articles for article (PubMed ID: 24910412)

  • 1. Efficient refolding and immobilization of PMMA-tag-fused single-chain Fv antibodies for sensitive immunological detection on a PMMA plate.
    Kumada Y; Ishikawa Y; Fujiwara Y; Takeda R; Miyamoto R; Niwa D; Momose S; Kang B; Kishimoto M
    J Immunol Methods; 2014 Sep; 411():1-10. PubMed ID: 24910412
    [TBL] [Abstract][Full Text] [Related]  

  • 2. Efficient preparation and site-directed immobilization of VHH antibodies by genetic fusion of poly(methylmethacrylate)-binding peptide (PMMA-Tag).
    Kumada Y; Kang B; Yamakawa K; Kishimoto M; Horiuchi J
    Biotechnol Prog; 2015; 31(6):1563-70. PubMed ID: 26399196
    [TBL] [Abstract][Full Text] [Related]  

  • 3. Improved lectin ELISA for glycosylation analysis of biomarkers using PS-tag-fused single-chain Fv.
    Kumada Y; Ohigashi Y; Emori Y; Imamura K; Omura Y; Kishimoto M
    J Immunol Methods; 2012 Nov; 385(1-2):15-22. PubMed ID: 22884622
    [TBL] [Abstract][Full Text] [Related]  

  • 4. Direct immobilization of functional single-chain variable fragment antibodies (scFvs) onto a polystyrene plate by genetic fusion of a polystyrene-binding peptide (PS-tag).
    Kumada Y; Hamasaki K; Shiritani Y; Nakagawa A; Kuroki D; Ohse T; Choi DH; Katakura Y; Kishimoto M
    Anal Bioanal Chem; 2009 Oct; 395(3):759-65. PubMed ID: 19680637
    [TBL] [Abstract][Full Text] [Related]  

  • 5. Novel solid-phase refolding method for preparation of scFv-immobilized polystyrene plates with high-antigen-binding activity.
    Kumada Y; Shiritani Y; Hamasaki K; Nakagawa A; Sasaki E; Kishimoto M
    Anal Bioanal Chem; 2010 Oct; 398(3):1295-303. PubMed ID: 20661728
    [TBL] [Abstract][Full Text] [Related]  

  • 6. Design and site-directed immobilization of single-chain Fv antibody to polystyrene latex beads via material-binding peptides and application to latex turbidimetric assay.
    Kumada Y; Miyamura Y; Tanibata R; Takahashi K; Ogasawara S; Gondaira F; Horiuchi JI
    J Biosci Bioeng; 2021 Jan; 131(1):84-89. PubMed ID: 33023860
    [TBL] [Abstract][Full Text] [Related]  

  • 7. Immobilization and functional reconstitution of antibody Fab fragment by solid-phase refolding.
    Kumada Y; Hamasaki K; Nakagawa A; Sasaki E; Shirai T; Okumura M; Inoue M; Kishimoto M
    J Immunol Methods; 2013 Dec; 400-401():70-7. PubMed ID: 24177435
    [TBL] [Abstract][Full Text] [Related]  

  • 8. Efficient immobilization of a ligand antibody with high antigen-binding activity by use of a polystyrene-binding peptide and an intelligent microtiter plate.
    Kumada Y; Hamasaki K; Shiritani Y; Ohse T; Kishimoto M
    J Biotechnol; 2009 Jun; 142(2):135-41. PubMed ID: 19501265
    [TBL] [Abstract][Full Text] [Related]  

  • 9. Preparation of scFv-immobilized quartz crystal microbalance sensor by PS-tag-mediated solid-phase refolding.
    Kumada Y; Sasaki E; Kishimoto M
    J Biosci Bioeng; 2011 Apr; 111(4):459-64. PubMed ID: 21215695
    [TBL] [Abstract][Full Text] [Related]  

  • 10. Screening of PC and PMMA-binding peptides for site-specific immobilization of proteins.
    Kumada Y; Murata S; Ishikawa Y; Nakatsuka K; Kishimoto M
    J Biotechnol; 2012 Aug; 160(3-4):222-8. PubMed ID: 22426519
    [TBL] [Abstract][Full Text] [Related]  

  • 11. Development of a one-step ELISA method using an affinity peptide tag specific to a hydrophilic polystyrene surface.
    Kumada Y; Katoh S; Imanaka H; Imamura K; Nakanishi K
    J Biotechnol; 2007 Jan; 127(2):288-99. PubMed ID: 16950537
    [TBL] [Abstract][Full Text] [Related]  

  • 12. Cloning, expression and efficient refolding of carbohydrate-peptide mimicry recognizing single chain antibody 2D10.
    Tapryal S; Krishnan L; Batra JK; Kaur KJ; Salunke DM
    Protein Expr Purif; 2010 Aug; 72(2):162-8. PubMed ID: 20363331
    [TBL] [Abstract][Full Text] [Related]  

  • 13. A combined strategy improves the solubility of aggregation-prone single-chain variable fragment antibodies.
    Sun W; Xie J; Lin H; Mi S; Li Z; Hua F; Hu Z
    Protein Expr Purif; 2012 May; 83(1):21-9. PubMed ID: 22387083
    [TBL] [Abstract][Full Text] [Related]  

  • 14. Expression and purification of a novel therapeutic single-chain variable fragment antibody against BNP from inclusion bodies of Escherichia coli.
    Bu D; Zhou Y; Tang J; Jing F; Zhang W
    Protein Expr Purif; 2013 Dec; 92(2):203-7. PubMed ID: 24128692
    [TBL] [Abstract][Full Text] [Related]  

  • 15. High-throughput, high-level production of PS-tag-fused single-chain Fvs by microplate-based culture.
    Kumada Y; Takase Y; Sasaki E; Kishimoto M
    J Biosci Bioeng; 2011 May; 111(5):569-73. PubMed ID: 21324737
    [TBL] [Abstract][Full Text] [Related]  

  • 16. [Evaluation of three in-vitro refolding methods for human-derived anti-CTLA4 scFv expressed in E. coli].
    Huang Q; Chen L; Zeng L; Wan L; Li S; Lu X; Cheng J
    Sheng Wu Yi Xue Gong Cheng Xue Za Zhi; 2006 Apr; 23(2):388-91. PubMed ID: 16706373
    [TBL] [Abstract][Full Text] [Related]  

  • 17. Characterization of polystyrene-binding peptides (PS-tags) for site-specific immobilization of proteins.
    Kumada Y; Kuroki D; Yasui H; Ohse T; Kishimoto M
    J Biosci Bioeng; 2010 Jun; 109(6):583-7. PubMed ID: 20471598
    [TBL] [Abstract][Full Text] [Related]  

  • 18. Generation of AcGFP fusion with single-chain Fv selected from a phage display library constructed from mice hyperimmunized against 5-methyl 2'-deoxycytidine.
    Ohshima M; Inoue K; Hayashi H; Tsuji D; Mizugaki M; Itoh K
    Protein Eng Des Sel; 2010 Nov; 23(11):881-8. PubMed ID: 20876190
    [TBL] [Abstract][Full Text] [Related]  

  • 19. Well-oriented ZZ-PS-tag with high Fc-binding onto polystyrene surface for controlled immobilization of capture antibodies.
    Tang JB; Sun XF; Yang HM; Zhang BG; Li ZJ; Lin ZJ; Gao ZQ
    Anal Chim Acta; 2013 May; 776():74-8. PubMed ID: 23601284
    [TBL] [Abstract][Full Text] [Related]  

  • 20. Development of a biotin mimic tagged ScFv antibody against western equine encephalitis virus: bacterial expression and refolding.
    Das D; Kriangkum J; Nagata LP; Fulton RE; Suresh MR
    J Virol Methods; 2004 May; 117(2):169-77. PubMed ID: 15041214
    [TBL] [Abstract][Full Text] [Related]  

    [Next]    [New Search]
    of 17.