144 related articles for article (PubMed ID: 2499352)
1. Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutants.
Geiger R; Rüter T; Alves J; Fliess A; Wolfes H; Pingoud V; Urbanke C; Maass G; Pingoud A; Düsterhöft A
Biochemistry; 1989 Mar; 28(6):2667-77. PubMed ID: 2499352
[TBL] [Abstract][Full Text] [Related]
2. Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymatic activity, not, however, the preference of this restriction endonuclease for cleavage within the site-GAATTC-.
Alves J; Rüter T; Geiger R; Fliess A; Maass G; Pingoud A
Biochemistry; 1989 Mar; 28(6):2678-84. PubMed ID: 2659077
[TBL] [Abstract][Full Text] [Related]
3. Probing the function of individual amino acid residues in the DNA binding site of the EcoRI restriction endonuclease by analysing the toxicity of genetically engineered mutants.
Oelgeschläger T; Geiger R; Rüter T; Alves J; Fliess A; Pingoud A
Gene; 1990 Apr; 89(1):19-27. PubMed ID: 2142667
[TBL] [Abstract][Full Text] [Related]
4. Clustering of null mutations in the EcoRI endonuclease.
Yanofsky SD; Love R; McClarin JA; Rosenberg JM; Boyer HW; Greene PJ
Proteins; 1987; 2(4):273-82. PubMed ID: 2834717
[TBL] [Abstract][Full Text] [Related]
5. Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition.
Heitman J; Model P
EMBO J; 1990 Oct; 9(10):3369-78. PubMed ID: 2209548
[TBL] [Abstract][Full Text] [Related]
6. Mutational analysis of the function of Gln115 in the EcoRI restriction endonuclease, a critical amino acid for recognition of the inner thymidine residue in the sequence -GAATTC- and for coupling specific DNA binding to catalysis.
Jeltsch A; Alves J; Oelgeschläger T; Wolfes H; Maass G; Pingoud A
J Mol Biol; 1993 Jan; 229(1):221-34. PubMed ID: 8421302
[TBL] [Abstract][Full Text] [Related]
7. Asn141 is essential for DNA recognition by EcoRI restriction endonuclease.
Fritz A; Küster W; Alves J
FEBS Lett; 1998 Oct; 438(1-2):66-70. PubMed ID: 9821960
[TBL] [Abstract][Full Text] [Related]
8. Combinatorial mutagenesis of three major groove-contacting residues of EcoRI: single and double amino acid replacements retaining methyltransferase-sensitive activities.
Osuna J; Flores H; Soberón X
Gene; 1991 Sep; 106(1):7-12. PubMed ID: 1937043
[TBL] [Abstract][Full Text] [Related]
9. Glu-111 is required for activation of the DNA cleavage center of EcoRI endonuclease.
King K; Benkovic SJ; Modrich P
J Biol Chem; 1989 Jul; 264(20):11807-15. PubMed ID: 2745417
[TBL] [Abstract][Full Text] [Related]
10. Determinants of EcoRI endonuclease sequence discrimination.
Needels MC; Fried SR; Love R; Rosenberg JM; Boyer HW; Greene PJ
Proc Natl Acad Sci U S A; 1989 May; 86(10):3579-83. PubMed ID: 2657723
[TBL] [Abstract][Full Text] [Related]
11. Thermodynamic and kinetic basis for the relaxed DNA sequence specificity of "promiscuous" mutant EcoRI endonucleases.
Sapienza PJ; Dela Torre CA; McCoy WH; Jana SV; Jen-Jacobson L
J Mol Biol; 2005 Apr; 348(2):307-24. PubMed ID: 15811370
[TBL] [Abstract][Full Text] [Related]
12. Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI.
Wolfes H; Alves J; Fliess A; Geiger R; Pingoud A
Nucleic Acids Res; 1986 Nov; 14(22):9063-80. PubMed ID: 3024128
[TBL] [Abstract][Full Text] [Related]
13. Temperature-sensitive mutants of the EcoRI endonuclease.
Muir RS; Flores H; Zinder ND; Model P; Soberon X; Heitman J
J Mol Biol; 1997 Dec; 274(5):722-37. PubMed ID: 9405154
[TBL] [Abstract][Full Text] [Related]
14. Asp-59 is not important for the catalytic activity of the restriction endonuclease EcoRI.
Grabowski G; Maass G; Alves J
FEBS Lett; 1996 Feb; 381(1-2):106-10. PubMed ID: 8641414
[TBL] [Abstract][Full Text] [Related]
15. Saturation mutagenesis of His114 of EcoRI reveals relaxed-specificity mutants.
Flores H; Osuna J; Heitman J; Soberón X
Gene; 1995 May; 157(1-2):295-301. PubMed ID: 7607511
[TBL] [Abstract][Full Text] [Related]
16. Structural and thermodynamic basis for enhanced DNA binding by a promiscuous mutant EcoRI endonuclease.
Sapienza PJ; Rosenberg JM; Jen-Jacobson L
Structure; 2007 Nov; 15(11):1368-82. PubMed ID: 17997963
[TBL] [Abstract][Full Text] [Related]
17. Structure-based redesign of the catalytic/metal binding site of Cfr10I restriction endonuclease reveals importance of spatial rather than sequence conservation of active centre residues.
Skirgaila R; Grazulis S; Bozic D; Huber R; Siksnys V
J Mol Biol; 1998 Jun; 279(2):473-81. PubMed ID: 9642051
[TBL] [Abstract][Full Text] [Related]
18. Pausing of the restriction endonuclease EcoRI during linear diffusion on DNA.
Jeltsch A; Alves J; Wolfes H; Maass G; Pingoud A
Biochemistry; 1994 Aug; 33(34):10215-9. PubMed ID: 8068662
[TBL] [Abstract][Full Text] [Related]
19. Substrate recognition by the EcoRI endonuclease.
Heitman J; Model P
Proteins; 1990; 7(2):185-97. PubMed ID: 2139225
[TBL] [Abstract][Full Text] [Related]
20. How the EcoRI endonuclease recognizes and cleaves DNA.
Heitman J
Bioessays; 1992 Jul; 14(7):445-54. PubMed ID: 1445286
[TBL] [Abstract][Full Text] [Related]
[Next] [New Search]
