These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
3. Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification. Oeemig JS; Aranko AS; Djupsjöbacka J; Heinämäki K; Iwaï H FEBS Lett; 2009 May; 583(9):1451-6. PubMed ID: 19344715 [TBL] [Abstract][Full Text] [Related]
4. The NMR structure of the engineered halophilic DnaE intein for segmental isotopic labeling using conditional protein splicing. Heikkinen HA; Aranko AS; Iwaï H J Magn Reson; 2022 May; 338():107195. PubMed ID: 35398651 [TBL] [Abstract][Full Text] [Related]
5. Segmental Isotope Labeling of Insoluble Proteins for Solid-State NMR by Protein Trans-Splicing. Schubeis T; Nagaraj M; Ritter C Methods Mol Biol; 2017; 1495():147-160. PubMed ID: 27714615 [TBL] [Abstract][Full Text] [Related]
6. Conserved residues that modulate protein trans-splicing of Npu DnaE split intein. Wu Q; Gao Z; Wei Y; Ma G; Zheng Y; Dong Y; Liu Y Biochem J; 2014 Jul; 461(2):247-55. PubMed ID: 24758175 [TBL] [Abstract][Full Text] [Related]
7. A streamlined method for preparing split intein for NMR study. Lee YZ; Lee YT; Lin YJ; Chen YJ; Sue SC Protein Expr Purif; 2014 Jul; 99():106-12. PubMed ID: 24751877 [TBL] [Abstract][Full Text] [Related]
8. Traceless splicing enabled by substrate-induced activation of the Nostoc punctiforme Npu DnaE intein after mutation of a catalytic cysteine to serine. Cheriyan M; Chan SH; Perler F J Mol Biol; 2014 Dec; 426(24):4018-4029. PubMed ID: 25451033 [TBL] [Abstract][Full Text] [Related]
9. In vivo and in vitro protein ligation by naturally occurring and engineered split DnaE inteins. Aranko AS; Züger S; Buchinger E; Iwaï H PLoS One; 2009; 4(4):e5185. PubMed ID: 19365564 [TBL] [Abstract][Full Text] [Related]
10. Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. Iwai H; Züger S; Jin J; Tam PH FEBS Lett; 2006 Mar; 580(7):1853-8. PubMed ID: 16516207 [TBL] [Abstract][Full Text] [Related]
11. Segmental isotopic labeling of HIV-1 capsid protein assemblies for solid state NMR. Gupta S; Tycko R J Biomol NMR; 2018 Feb; 70(2):103-114. PubMed ID: 29464399 [TBL] [Abstract][Full Text] [Related]
12. Split intein mediated ultra-rapid purification of tagless protein (SIRP). Guan D; Ramirez M; Chen Z Biotechnol Bioeng; 2013 Sep; 110(9):2471-81. PubMed ID: 23568256 [TBL] [Abstract][Full Text] [Related]
13. Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein. Busche AE; Aranko AS; Talebzadeh-Farooji M; Bernhard F; Dötsch V; Iwaï H Angew Chem Int Ed Engl; 2009; 48(33):6128-31. PubMed ID: 19591176 [No Abstract] [Full Text] [Related]
14. Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies. Züger S; Iwai H Nat Biotechnol; 2005 Jun; 23(6):736-40. PubMed ID: 15908942 [TBL] [Abstract][Full Text] [Related]
15. Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro. Muona M; Aranko AS; Raulinaitis V; Iwaï H Nat Protoc; 2010 Mar; 5(3):574-87. PubMed ID: 20203672 [TBL] [Abstract][Full Text] [Related]
16. A Split-Intein-Based Method for the Efficient Production of Circularized Nanodiscs for Structural Studies of Membrane Proteins. Miehling J; Goricanec D; Hagn F Chembiochem; 2018 Sep; 19(18):1927-1933. PubMed ID: 29947468 [TBL] [Abstract][Full Text] [Related]
17. Faster protein splicing with the Nostoc punctiforme DnaE intein using non-native extein residues. Cheriyan M; Pedamallu CS; Tori K; Perler F J Biol Chem; 2013 Mar; 288(9):6202-11. PubMed ID: 23306197 [TBL] [Abstract][Full Text] [Related]
18. Functional characterization of a naturally occurring trans-splicing intein from Synechococcus elongatus in a mammalian cell system. Chen L; Zhang Y; Li G; Huang H; Zhou N Anal Biochem; 2010 Dec; 407(2):180-7. PubMed ID: 20727340 [TBL] [Abstract][Full Text] [Related]
19. Engineering split intein DnaE from Nostoc punctiforme for rapid protein purification. Ramirez M; Valdes N; Guan D; Chen Z Protein Eng Des Sel; 2013 Mar; 26(3):215-23. PubMed ID: 23223807 [TBL] [Abstract][Full Text] [Related]
20. Segmental isotopic labeling of proteins for nuclear magnetic resonance. Liu D; Xu R; Cowburn D Methods Enzymol; 2009; 462():151-75. PubMed ID: 19632474 [TBL] [Abstract][Full Text] [Related] [Next] [New Search]