BIOMARKERS

Molecular Biopsy of Human Tumors

- a resource for Precision Medicine *

154 related articles for article (PubMed ID: 25449543)

  • 1. DC-Analyzer-facilitated combinatorial strategy for rapid directed evolution of functional enzymes with multiple mutagenesis sites.
    Wang X; Zheng K; Zheng H; Nie H; Yang Z; Tang L
    J Biotechnol; 2014 Dec; 192 Pt A():102-7. PubMed ID: 25449543
    [TBL] [Abstract][Full Text] [Related]  

  • 2. MDC-Analyzer-facilitated combinatorial strategy for improving the activity and stability of halohydrin dehalogenase from Agrobacterium radiobacter AD1.
    Wang X; Lin H; Zheng Y; Feng J; Yang Z; Tang L
    J Biotechnol; 2015 Jul; 206():1-7. PubMed ID: 25896949
    [TBL] [Abstract][Full Text] [Related]  

  • 3. Exploring the thermostable properties of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by a combinatorial directed evolution strategy.
    Wu Z; Deng W; Tong Y; Liao Q; Xin D; Yu H; Feng J; Tang L
    Appl Microbiol Biotechnol; 2017 Apr; 101(8):3201-3211. PubMed ID: 28074221
    [TBL] [Abstract][Full Text] [Related]  

  • 4. Improvement of the thermostability and activity of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by engineering C-terminal amino acids.
    Wang X; Han S; Yang Z; Tang L
    J Biotechnol; 2015 Oct; 212():92-8. PubMed ID: 26302840
    [TBL] [Abstract][Full Text] [Related]  

  • 5. Enhancement of the thermostability of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by constructing a combinatorial smart library.
    Wang X; Xie Z; Yan J; He X; Liu W; Sun Y
    Int J Biol Macromol; 2019 Jun; 130():19-23. PubMed ID: 30790639
    [TBL] [Abstract][Full Text] [Related]  

  • 6. Iterative saturation mutagenesis: a powerful approach to engineer proteins by systematically simulating Darwinian evolution.
    Acevedo-Rocha CG; Hoebenreich S; Reetz MT
    Methods Mol Biol; 2014; 1179():103-28. PubMed ID: 25055773
    [TBL] [Abstract][Full Text] [Related]  

  • 7. Exploring the enantioselective mechanism of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by iterative saturation mutagenesis.
    Guo C; Chen Y; Zheng Y; Zhang W; Tao Y; Feng J; Tang L
    Appl Environ Microbiol; 2015 Apr; 81(8):2919-26. PubMed ID: 25681194
    [TBL] [Abstract][Full Text] [Related]  

  • 8. A high-throughput colorimetric assay for screening halohydrin dehalogenase saturation mutagenesis libraries.
    Tang L; Li Y; Wang X
    J Biotechnol; 2010 Jun; 147(3-4):164-8. PubMed ID: 20399816
    [TBL] [Abstract][Full Text] [Related]  

  • 9. Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes.
    Reetz MT; Carballeira JD
    Nat Protoc; 2007; 2(4):891-903. PubMed ID: 17446890
    [TBL] [Abstract][Full Text] [Related]  

  • 10. Enhanced catalytic efficiency and enantioselectivity of epoxide hydrolase from Agrobacterium radiobacter AD1 by iterative saturation mutagenesis for (R)-epichlorohydrin synthesis.
    Zou SP; Zheng YG; Wu Q; Wang ZC; Xue YP; Liu ZQ
    Appl Microbiol Biotechnol; 2018 Jan; 102(2):733-742. PubMed ID: 29151159
    [TBL] [Abstract][Full Text] [Related]  

  • 11. Directed evolution by using iterative saturation mutagenesis based on multiresidue sites.
    Parra LP; Agudo R; Reetz MT
    Chembiochem; 2013 Nov; 14(17):2301-9. PubMed ID: 24136881
    [TBL] [Abstract][Full Text] [Related]  

  • 12. Clarifying the Difference between Iterative Saturation Mutagenesis as a Rational Guide in Directed Evolution and OmniChange as a Gene Mutagenesis Technique.
    Acevedo-Rocha CG; Sun Z; Reetz MT
    Chembiochem; 2018 Dec; 19(24):2542-2544. PubMed ID: 30408315
    [TBL] [Abstract][Full Text] [Related]  

  • 13. Construction of "small-intelligent" focused mutagenesis libraries using well-designed combinatorial degenerate primers.
    Tang L; Gao H; Zhu X; Wang X; Zhou M; Jiang R
    Biotechniques; 2012 Mar; 52(3):149-58. PubMed ID: 22401547
    [TBL] [Abstract][Full Text] [Related]  

  • 14. Steady-state kinetics and tryptophan fluorescence properties of halohydrin dehalogenase from Agrobacterium radiobacter. Roles of W139 and W249 in the active site and halide-induced conformational change.
    Tang L; van Merode AE; Lutje Spelberg JH; Fraaije MW; Janssen DB
    Biochemistry; 2003 Dec; 42(47):14057-65. PubMed ID: 14636074
    [TBL] [Abstract][Full Text] [Related]  

  • 15. Nitrite-mediated synthesis of chiral epichlorohydrin using halohydrin dehalogenase from Agrobacterium radiobacter AD1.
    Jin HX; Hu ZC; Liu ZQ; Zheng YG
    Biotechnol Appl Biochem; 2012; 59(3):170-7. PubMed ID: 23586826
    [TBL] [Abstract][Full Text] [Related]  

  • 16. Improved catalytic properties of halohydrin dehalogenase by modification of the halide-binding site.
    Tang L; Torres Pazmiño DE; Fraaije MW; de Jong RM; Dijkstra BW; Janssen DB
    Biochemistry; 2005 May; 44(17):6609-18. PubMed ID: 15850394
    [TBL] [Abstract][Full Text] [Related]  

  • 17. Enhancing the thermal robustness of an enzyme by directed evolution: least favorable starting points and inferior mutants can map superior evolutionary pathways.
    Gumulya Y; Reetz MT
    Chembiochem; 2011 Nov; 12(16):2502-10. PubMed ID: 21913300
    [TBL] [Abstract][Full Text] [Related]  

  • 18. Enhancing the biocatalytic manufacture of the key intermediate of atorvastatin by focused directed evolution of halohydrin dehalogenase.
    Luo Y; Chen Y; Ma H; Tian Z; Zhang Y; Zhang J
    Sci Rep; 2017 Feb; 7():42064. PubMed ID: 28165015
    [TBL] [Abstract][Full Text] [Related]  

  • 19. Enhancing the recombinant protein expression of halohydrin dehalogenase HheA in Escherichia coli by applying a codon optimization strategy.
    Tang L; Jiang R; Zheng K; Zhu X
    Enzyme Microb Technol; 2011 Sep; 49(4):395-401. PubMed ID: 22112566
    [TBL] [Abstract][Full Text] [Related]  

  • 20. A single point mutation enhances hydroxynitrile synthesis by halohydrin dehalogenase.
    Schallmey M; Jekel P; Tang L; Majerić Elenkov M; Höffken HW; Hauer B; Janssen DB
    Enzyme Microb Technol; 2015 Mar; 70():50-7. PubMed ID: 25659632
    [TBL] [Abstract][Full Text] [Related]  

    [Next]    [New Search]
    of 8.