BIOMARKERS

Molecular Biopsy of Human Tumors

- a resource for Precision Medicine *

167 related articles for article (PubMed ID: 2659077)

  • 1. Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymatic activity, not, however, the preference of this restriction endonuclease for cleavage within the site-GAATTC-.
    Alves J; Rüter T; Geiger R; Fliess A; Maass G; Pingoud A
    Biochemistry; 1989 Mar; 28(6):2678-84. PubMed ID: 2659077
    [TBL] [Abstract][Full Text] [Related]  

  • 2. Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutants.
    Geiger R; Rüter T; Alves J; Fliess A; Wolfes H; Pingoud V; Urbanke C; Maass G; Pingoud A; Düsterhöft A
    Biochemistry; 1989 Mar; 28(6):2667-77. PubMed ID: 2499352
    [TBL] [Abstract][Full Text] [Related]  

  • 3. Structure of the DNA-Eco RI endonuclease recognition complex at 3 A resolution.
    McClarin JA; Frederick CA; Wang BC; Greene P; Boyer HW; Grable J; Rosenberg JM
    Science; 1986 Dec; 234(4783):1526-41. PubMed ID: 3024321
    [TBL] [Abstract][Full Text] [Related]  

  • 4. Asn141 is essential for DNA recognition by EcoRI restriction endonuclease.
    Fritz A; Küster W; Alves J
    FEBS Lett; 1998 Oct; 438(1-2):66-70. PubMed ID: 9821960
    [TBL] [Abstract][Full Text] [Related]  

  • 5. Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition.
    Heitman J; Model P
    EMBO J; 1990 Oct; 9(10):3369-78. PubMed ID: 2209548
    [TBL] [Abstract][Full Text] [Related]  

  • 6. Thermodynamic and kinetic basis for the relaxed DNA sequence specificity of "promiscuous" mutant EcoRI endonucleases.
    Sapienza PJ; Dela Torre CA; McCoy WH; Jana SV; Jen-Jacobson L
    J Mol Biol; 2005 Apr; 348(2):307-24. PubMed ID: 15811370
    [TBL] [Abstract][Full Text] [Related]  

  • 7. Influence of enzyme-substrate contacts located outside the EcoRI recognition site on cleavage of duplex oligodeoxyribonucleotide substrates by EcoRI endonuclease.
    Van Cleve MD; Gumport RI
    Biochemistry; 1992 Jan; 31(2):334-9. PubMed ID: 1731891
    [TBL] [Abstract][Full Text] [Related]  

  • 8. A dodecapeptide comprising the extended chain-alpha 4 region of the restriction endonuclease EcoRI specifically binds to the EcoRI recognition site.
    Jeltsch A; Alves J; Urbanke C; Maass G; Eckstein H; Lianshan Z; Bayer E; Pingoud A
    J Biol Chem; 1995 Mar; 270(10):5122-9. PubMed ID: 7890621
    [TBL] [Abstract][Full Text] [Related]  

  • 9. Mutational analysis of the function of Gln115 in the EcoRI restriction endonuclease, a critical amino acid for recognition of the inner thymidine residue in the sequence -GAATTC- and for coupling specific DNA binding to catalysis.
    Jeltsch A; Alves J; Oelgeschläger T; Wolfes H; Maass G; Pingoud A
    J Mol Biol; 1993 Jan; 229(1):221-34. PubMed ID: 8421302
    [TBL] [Abstract][Full Text] [Related]  

  • 10. Saturation mutagenesis of His114 of EcoRI reveals relaxed-specificity mutants.
    Flores H; Osuna J; Heitman J; Soberón X
    Gene; 1995 May; 157(1-2):295-301. PubMed ID: 7607511
    [TBL] [Abstract][Full Text] [Related]  

  • 11. Determinants of EcoRI endonuclease sequence discrimination.
    Needels MC; Fried SR; Love R; Rosenberg JM; Boyer HW; Greene PJ
    Proc Natl Acad Sci U S A; 1989 May; 86(10):3579-83. PubMed ID: 2657723
    [TBL] [Abstract][Full Text] [Related]  

  • 12. How the EcoRI endonuclease recognizes and cleaves DNA.
    Heitman J
    Bioessays; 1992 Jul; 14(7):445-54. PubMed ID: 1445286
    [TBL] [Abstract][Full Text] [Related]  

  • 13. Asp-59 is not important for the catalytic activity of the restriction endonuclease EcoRI.
    Grabowski G; Maass G; Alves J
    FEBS Lett; 1996 Feb; 381(1-2):106-10. PubMed ID: 8641414
    [TBL] [Abstract][Full Text] [Related]  

  • 14. Site-directed mutagenesis in the catalytic center of the restriction endonuclease EcoRI.
    Grabowski G; Jeltsch A; Wolfes H; Maass G; Alves J
    Gene; 1995 May; 157(1-2):113-8. PubMed ID: 7607470
    [TBL] [Abstract][Full Text] [Related]  

  • 15. Probing the function of individual amino acid residues in the DNA binding site of the EcoRI restriction endonuclease by analysing the toxicity of genetically engineered mutants.
    Oelgeschläger T; Geiger R; Rüter T; Alves J; Fliess A; Pingoud A
    Gene; 1990 Apr; 89(1):19-27. PubMed ID: 2142667
    [TBL] [Abstract][Full Text] [Related]  

  • 16. Stereoselective interaction with chiral phosphorothioates at the central DNA kink of the EcoRI endonuclease-GAATTC complex.
    Lesser DR; Grajkowski A; Kurpiewski MR; Koziolkiewicz M; Stec WJ; Jen-Jacobson L
    J Biol Chem; 1992 Dec; 267(34):24810-8. PubMed ID: 1447218
    [TBL] [Abstract][Full Text] [Related]  

  • 17. 8-Oxoguanine Affects DNA Backbone Conformation in the EcoRI Recognition Site and Inhibits Its Cleavage by the Enzyme.
    Hoppins JJ; Gruber DR; Miears HL; Kiryutin AS; Kasymov RD; Petrova DV; Endutkin AV; Popov AV; Yurkovskaya AV; Fedechkin SO; Brockerman JA; Zharkov DO; Smirnov SL
    PLoS One; 2016; 11(10):e0164424. PubMed ID: 27749894
    [TBL] [Abstract][Full Text] [Related]  

  • 18. Structural and thermodynamic basis for enhanced DNA binding by a promiscuous mutant EcoRI endonuclease.
    Sapienza PJ; Rosenberg JM; Jen-Jacobson L
    Structure; 2007 Nov; 15(11):1368-82. PubMed ID: 17997963
    [TBL] [Abstract][Full Text] [Related]  

  • 19. Structure-based redesign of the catalytic/metal binding site of Cfr10I restriction endonuclease reveals importance of spatial rather than sequence conservation of active centre residues.
    Skirgaila R; Grazulis S; Bozic D; Huber R; Siksnys V
    J Mol Biol; 1998 Jun; 279(2):473-81. PubMed ID: 9642051
    [TBL] [Abstract][Full Text] [Related]  

  • 20. Sequence context influencing cleavage activity of the K130E mutant of the restriction endonuclease EcoRI identified by a site selection assay.
    Windolph S; Fritz A; Oelgeschläger T; Wolfes H; Alves J
    Biochemistry; 1997 Aug; 36(31):9478-85. PubMed ID: 9235992
    [TBL] [Abstract][Full Text] [Related]  

    [Next]    [New Search]
    of 9.