These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
3. Efficient genome editing by FACS enrichment of paired D10A Cas9 nickases coupled with fluorescent proteins. Gopalappa R; Song M; Chandrasekaran AP; Das S; Haq S; Koh HC; Ramakrishna S Arch Pharm Res; 2018 Sep; 41(9):911-920. PubMed ID: 29855892 [TBL] [Abstract][Full Text] [Related]
4. The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny. Schiml S; Fauser F; Puchta H Plant J; 2014 Dec; 80(6):1139-50. PubMed ID: 25327456 [TBL] [Abstract][Full Text] [Related]
5. Target-dependent nickase activities of the CRISPR-Cas nucleases Cpf1 and Cas9. Fu BXH; Smith JD; Fuchs RT; Mabuchi M; Curcuru J; Robb GB; Fire AZ Nat Microbiol; 2019 May; 4(5):888-897. PubMed ID: 30833733 [TBL] [Abstract][Full Text] [Related]
6. CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases. Schiml S; Fauser F; Puchta H Methods Mol Biol; 2016; 1469():111-22. PubMed ID: 27557689 [TBL] [Abstract][Full Text] [Related]
7. Multigene knockout utilizing off-target mutations of the CRISPR/Cas9 system in rice. Endo M; Mikami M; Toki S Plant Cell Physiol; 2015 Jan; 56(1):41-7. PubMed ID: 25392068 [TBL] [Abstract][Full Text] [Related]
9. Developing Heritable Mutations in Arabidopsis thaliana Using a Modified CRISPR/Cas9 Toolkit Comprising PAM-Altered Cas9 Variants and gRNAs. Yamamoto A; Ishida T; Yoshimura M; Kimura Y; Sawa S Plant Cell Physiol; 2019 Oct; 60(10):2255-2262. PubMed ID: 31198958 [TBL] [Abstract][Full Text] [Related]
10. Parameters affecting frequency of CRISPR/Cas9 mediated targeted mutagenesis in rice. Mikami M; Toki S; Endo M Plant Cell Rep; 2015 Oct; 34(10):1807-15. PubMed ID: 26134856 [TBL] [Abstract][Full Text] [Related]
11. Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs. Fu Y; Reyon D; Joung JK Methods Enzymol; 2014; 546():21-45. PubMed ID: 25398334 [TBL] [Abstract][Full Text] [Related]
12. A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice. Tang X; Liu G; Zhou J; Ren Q; You Q; Tian L; Xin X; Zhong Z; Liu B; Zheng X; Zhang D; Malzahn A; Gong Z; Qi Y; Zhang T; Zhang Y Genome Biol; 2018 Jul; 19(1):84. PubMed ID: 29973285 [TBL] [Abstract][Full Text] [Related]
13. Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases. Cho SW; Kim S; Kim Y; Kweon J; Kim HS; Bae S; Kim JS Genome Res; 2014 Jan; 24(1):132-41. PubMed ID: 24253446 [TBL] [Abstract][Full Text] [Related]
14. Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize. Lee K; Zhang Y; Kleinstiver BP; Guo JA; Aryee MJ; Miller J; Malzahn A; Zarecor S; Lawrence-Dill CJ; Joung JK; Qi Y; Wang K Plant Biotechnol J; 2019 Feb; 17(2):362-372. PubMed ID: 29972722 [TBL] [Abstract][Full Text] [Related]
15. Multiplex nucleotide editing by high-fidelity Cas9 variants with improved efficiency in rice. Xu W; Song W; Yang Y; Wu Y; Lv X; Yuan S; Liu Y; Yang J BMC Plant Biol; 2019 Nov; 19(1):511. PubMed ID: 31752697 [TBL] [Abstract][Full Text] [Related]
16. Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Fauser F; Schiml S; Puchta H Plant J; 2014 Jul; 79(2):348-59. PubMed ID: 24836556 [TBL] [Abstract][Full Text] [Related]
17. CRISPR Genome Editing Made Easy Through the CHOPCHOP Website. Labun K; Krause M; Torres Cleuren Y; Valen E Curr Protoc; 2021 Apr; 1(4):e46. PubMed ID: 33905612 [TBL] [Abstract][Full Text] [Related]
18. RNA-guided genome editing in plants using a CRISPR-Cas system. Xie K; Yang Y Mol Plant; 2013 Nov; 6(6):1975-83. PubMed ID: 23956122 [TBL] [Abstract][Full Text] [Related]
19. Targeted mutagenesis in cotton (Gossypium hirsutum L.) using the CRISPR/Cas9 system. Chen X; Lu X; Shu N; Wang S; Wang J; Wang D; Guo L; Ye W Sci Rep; 2017 Mar; 7():44304. PubMed ID: 28287154 [TBL] [Abstract][Full Text] [Related]
20. An efficient DNA- and selectable-marker-free genome-editing system using zygotes in rice. Toda E; Koiso N; Takebayashi A; Ichikawa M; Kiba T; Osakabe K; Osakabe Y; Sakakibara H; Kato N; Okamoto T Nat Plants; 2019 Apr; 5(4):363-368. PubMed ID: 30911123 [TBL] [Abstract][Full Text] [Related] [Next] [New Search]