These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


BIOMARKERS

Molecular Biopsy of Human Tumors

- a resource for Precision Medicine *

205 related articles for article (PubMed ID: 27709589)

  • 1. Revised Mechanism and Improved Efficiency of the QuikChange Site-Directed Mutagenesis Method.
    Xia Y; Xun L
    Methods Mol Biol; 2017; 1498():367-374. PubMed ID: 27709589
    [TBL] [Abstract][Full Text] [Related]  

  • 2. New insights into the QuikChange™ process guide the use of Phusion DNA polymerase for site-directed mutagenesis.
    Xia Y; Chu W; Qi Q; Xun L
    Nucleic Acids Res; 2015 Jan; 43(2):e12. PubMed ID: 25399421
    [TBL] [Abstract][Full Text] [Related]  

  • 3. A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method.
    Tseng WC; Lin JW; Wei TY; Fang TY
    Anal Biochem; 2008 Apr; 375(2):376-8. PubMed ID: 18198125
    [TBL] [Abstract][Full Text] [Related]  

  • 4. Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange Site-Directed Mutagenesis.
    Wang W; Malcolm BA
    Biotechniques; 1999 Apr; 26(4):680-2. PubMed ID: 10343905
    [TBL] [Abstract][Full Text] [Related]  

  • 5. Multichange isothermal mutagenesis: a new strategy for multiple site-directed mutations in plasmid DNA.
    Mitchell LA; Cai Y; Taylor M; Noronha AM; Chuang J; Dai L; Boeke JD
    ACS Synth Biol; 2013 Aug; 2(8):473-7. PubMed ID: 23654272
    [TBL] [Abstract][Full Text] [Related]  

  • 6. An efficient approach for site-directed mutagenesis using central overlapping primers.
    Wang H; Zhou N; Ding F; Li Z; Chen R; Han A; Liu R
    Anal Biochem; 2011 Nov; 418(2):304-6. PubMed ID: 21803022
    [TBL] [Abstract][Full Text] [Related]  

  • 7. An In Vitro Single-Primer Site-Directed Mutagenesis Method for Use in Biotechnology.
    Huang Y; Zhang L
    Methods Mol Biol; 2017; 1498():375-383. PubMed ID: 27709590
    [TBL] [Abstract][Full Text] [Related]  

  • 8. Facile Site-Directed Mutagenesis of Large Constructs Using Gibson Isothermal DNA Assembly.
    Yonemoto IT; Weyman PD
    Methods Mol Biol; 2017; 1498():359-366. PubMed ID: 27709588
    [TBL] [Abstract][Full Text] [Related]  

  • 9. Improved thermostability and PCR efficiency of Thermococcus celericrescens DNA polymerase via site-directed mutagenesis.
    Kim KP; Cho SS; Lee KK; Youn MH; Kwon ST
    J Biotechnol; 2011 Sep; 155(2):156-63. PubMed ID: 21723333
    [TBL] [Abstract][Full Text] [Related]  

  • 10. Two-stage polymerase chain reaction protocol allowing introduction of multiple mutations, deletions, and insertions, using QuikChange site-directed mutagenesis.
    Wang W; Malcolm BA
    Methods Mol Biol; 2002; 182():37-43. PubMed ID: 11768973
    [No Abstract]   [Full Text] [Related]  

  • 11. Improved PCR method for high-efficiency site-directed mutagenesis using class 2S restriction enzymes.
    Vilardaga JP; Di Paolo E; Bollen A
    Biotechniques; 1995 Apr; 18(4):604-6. PubMed ID: 7598888
    [No Abstract]   [Full Text] [Related]  

  • 12. An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol.
    Liu H; Naismith JH
    BMC Biotechnol; 2008 Dec; 8():91. PubMed ID: 19055817
    [TBL] [Abstract][Full Text] [Related]  

  • 13. A one-step PCR-based method for rapid and efficient site-directed fragment deletion, insertion, and substitution mutagenesis.
    Qi D; Scholthof KB
    J Virol Methods; 2008 Apr; 149(1):85-90. PubMed ID: 18314204
    [TBL] [Abstract][Full Text] [Related]  

  • 14. Directed mutagenesis using the polymerase chain reaction.
    Cormack B
    Curr Protoc Neurosci; 2001 May; Chapter 4():Unit 4.11. PubMed ID: 18428475
    [TBL] [Abstract][Full Text] [Related]  

  • 15. Long distance multiple-site directed plasmid mutagenesis by one-step PCR using non-overlapped primers.
    Bu Y; Wang H; Li J; Zhang Z; Hu Y; Xu Z
    Mol Biotechnol; 2013 Sep; 55(1):49-53. PubMed ID: 23644994
    [TBL] [Abstract][Full Text] [Related]  

  • 16. A rapid and efficient method for multiple-site mutagenesis with a modified overlap extension PCR.
    An Y; Ji J; Wu W; Lv A; Huang R; Wei Y
    Appl Microbiol Biotechnol; 2005 Oct; 68(6):774-8. PubMed ID: 16249879
    [TBL] [Abstract][Full Text] [Related]  

  • 17. A fast and simple method for simultaneous mixed site-specific mutagenesis of a wide coding sequence.
    Follo C; Isidoro C
    Biotechnol Appl Biochem; 2008 Feb; 49(Pt 2):175-83. PubMed ID: 17640176
    [TBL] [Abstract][Full Text] [Related]  

  • 18. SORS: a universal one-round PCR-based method for site-directed mutagenesis.
    Wang BL; Quan JX; Liang H; Jiao YL; Li XX; Guo G; Zhang JY
    Mol Biotechnol; 2008 Sep; 40(1):69-75. PubMed ID: 18415714
    [TBL] [Abstract][Full Text] [Related]  

  • 19. QuikChange shuffling: a convenient and robust method for site-directed mutagenesis and random recombination of homologous genes.
    An Y; Chen L; Sun S; Lv A; Wu W
    N Biotechnol; 2011 Jul; 28(4):320-5. PubMed ID: 21402187
    [TBL] [Abstract][Full Text] [Related]  

  • 20. Gene splicing and mutagenesis by PCR-driven overlap extension.
    Heckman KL; Pease LR
    Nat Protoc; 2007; 2(4):924-32. PubMed ID: 17446874
    [TBL] [Abstract][Full Text] [Related]  

    [Next]    [New Search]
    of 11.