BIOMARKERS

Molecular Biopsy of Human Tumors

- a resource for Precision Medicine *

324 related articles for article (PubMed ID: 27894274)

  • 1. Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ.
    Aida T; Nakade S; Sakuma T; Izu Y; Oishi A; Mochida K; Ishikubo H; Usami T; Aizawa H; Yamamoto T; Tanaka K
    BMC Genomics; 2016 Nov; 17(1):979. PubMed ID: 27894274
    [TBL] [Abstract][Full Text] [Related]  

  • 2. Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids.
    Sakuma T; Takenaga M; Kawabe Y; Nakamura T; Kamihira M; Yamamoto T
    Int J Mol Sci; 2015 Oct; 16(10):23849-66. PubMed ID: 26473830
    [TBL] [Abstract][Full Text] [Related]  

  • 3. Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9.
    Nakade S; Tsubota T; Sakane Y; Kume S; Sakamoto N; Obara M; Daimon T; Sezutsu H; Yamamoto T; Sakuma T; Suzuki KT
    Nat Commun; 2014 Nov; 5():5560. PubMed ID: 25410609
    [TBL] [Abstract][Full Text] [Related]  

  • 4. One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes.
    Owen JR; Hennig SL; McNabb BR; Mansour TA; Smith JM; Lin JC; Young AE; Trott JF; Murray JD; Delany ME; Ross PJ; Van Eenennaam AL
    BMC Genomics; 2021 Feb; 22(1):118. PubMed ID: 33581720
    [TBL] [Abstract][Full Text] [Related]  

  • 5. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.
    Sakuma T; Nakade S; Sakane Y; Suzuki KT; Yamamoto T
    Nat Protoc; 2016 Jan; 11(1):118-33. PubMed ID: 26678082
    [TBL] [Abstract][Full Text] [Related]  

  • 6. ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes.
    Yoshimi K; Kunihiro Y; Kaneko T; Nagahora H; Voigt B; Mashimo T
    Nat Commun; 2016 Jan; 7():10431. PubMed ID: 26786405
    [TBL] [Abstract][Full Text] [Related]  

  • 7. Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO.
    Nakamae K; Nishimura Y; Takenaga M; Nakade S; Sakamoto N; Ide H; Sakuma T; Yamamoto T
    Bioengineered; 2017 May; 8(3):302-308. PubMed ID: 28453368
    [TBL] [Abstract][Full Text] [Related]  

  • 8. Highly efficient CRISPR/HDR-mediated knock-in for mouse embryonic stem cells and zygotes.
    Wang B; Li K; Wang A; Reiser M; Saunders T; Lockey RF; Wang JW
    Biotechniques; 2015 Oct; 59(4):201-2, 204, 206-8. PubMed ID: 26458548
    [TBL] [Abstract][Full Text] [Related]  

  • 9. Efficient targeted integration directed by short homology in zebrafish and mammalian cells.
    Wierson WA; Welker JM; Almeida MP; Mann CM; Webster DA; Torrie ME; Weiss TJ; Kambakam S; Vollbrecht MK; Lan M; McKeighan KC; Levey J; Ming Z; Wehmeier A; Mikelson CS; Haltom JA; Kwan KM; Chien CB; Balciunas D; Ekker SC; Clark KJ; Webber BR; Moriarity BS; Solin SL; Carlson DF; Dobbs DL; McGrail M; Essner J
    Elife; 2020 May; 9():. PubMed ID: 32412410
    [TBL] [Abstract][Full Text] [Related]  

  • 10. Homologous recombination-mediated targeted integration in monkey embryos using TALE nucleases.
    Chu C; Yang Z; Yang J; Yan L; Si C; Kang Y; Chen Z; Chen Y; Ji W; Niu Y
    BMC Biotechnol; 2019 Jan; 19(1):7. PubMed ID: 30646876
    [TBL] [Abstract][Full Text] [Related]  

  • 11. Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated, homology-independent knock-in system.
    Katoh Y; Michisaka S; Nozaki S; Funabashi T; Hirano T; Takei R; Nakayama K
    Mol Biol Cell; 2017 Apr; 28(7):898-906. PubMed ID: 28179459
    [TBL] [Abstract][Full Text] [Related]  

  • 12. A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair.
    Suzuki KT; Sakane Y; Suzuki M; Yamamoto T
    Methods Mol Biol; 2018; 1865():91-103. PubMed ID: 30151761
    [TBL] [Abstract][Full Text] [Related]  

  • 13. CRISPR/Cas9 and TALEN-mediated knock-in approaches in zebrafish.
    Auer TO; Del Bene F
    Methods; 2014 Sep; 69(2):142-50. PubMed ID: 24704174
    [TBL] [Abstract][Full Text] [Related]  

  • 14. Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice.
    Aida T; Chiyo K; Usami T; Ishikubo H; Imahashi R; Wada Y; Tanaka KF; Sakuma T; Yamamoto T; Tanaka K
    Genome Biol; 2015 Apr; 16(1):87. PubMed ID: 25924609
    [TBL] [Abstract][Full Text] [Related]  

  • 15. Homology arms of targeting vectors for gene insertions and CRISPR/Cas9 technology: size does not matter; quality control of targeted clones does.
    Petrezselyova S; Kinsky S; Truban D; Sedlacek R; Burtscher I; Lickert H
    Cell Mol Biol Lett; 2015 Dec; 20(5):773-87. PubMed ID: 26540224
    [TBL] [Abstract][Full Text] [Related]  

  • 16. Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems.
    Kawabe Y; Komatsu S; Komatsu S; Murakami M; Ito A; Sakuma T; Nakamura T; Yamamoto T; Kamihira M
    J Biosci Bioeng; 2018 May; 125(5):599-605. PubMed ID: 29295784
    [TBL] [Abstract][Full Text] [Related]  

  • 17. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency.
    Song J; Yang D; Xu J; Zhu T; Chen YE; Zhang J
    Nat Commun; 2016 Jan; 7():10548. PubMed ID: 26817820
    [TBL] [Abstract][Full Text] [Related]  

  • 18. Assembling the Streptococcus thermophilus clustered regularly interspaced short palindromic repeats (CRISPR) array for multiplex DNA targeting.
    Guo L; Xu K; Liu Z; Zhang C; Xin Y; Zhang Z
    Anal Biochem; 2015 Jun; 478():131-3. PubMed ID: 25748774
    [TBL] [Abstract][Full Text] [Related]  

  • 19. Intracellular generation of single-strand template increases the knock-in efficiency by combining CRISPR/Cas9 with AAV.
    Xiao Q; Min T; Ma S; Hu L; Chen H; Lu D
    Mol Genet Genomics; 2018 Aug; 293(4):1051-1060. PubMed ID: 29671068
    [TBL] [Abstract][Full Text] [Related]  

  • 20. CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci.
    Tasan I; Sustackova G; Zhang L; Kim J; Sivaguru M; HamediRad M; Wang Y; Genova J; Ma J; Belmont AS; Zhao H
    Nucleic Acids Res; 2018 Sep; 46(17):e100. PubMed ID: 29912475
    [TBL] [Abstract][Full Text] [Related]  

    [Next]    [New Search]
    of 17.