These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
7. Developing Heritable Mutations in Arabidopsis thaliana Using a Modified CRISPR/Cas9 Toolkit Comprising PAM-Altered Cas9 Variants and gRNAs. Yamamoto A; Ishida T; Yoshimura M; Kimura Y; Sawa S Plant Cell Physiol; 2019 Oct; 60(10):2255-2262. PubMed ID: 31198958 [TBL] [Abstract][Full Text] [Related]
8. DNA targeting by Clostridium cellulolyticum CRISPR-Cas9 Type II-C system. Fedorova I; Arseniev A; Selkova P; Pobegalov G; Goryanin I; Vasileva A; Musharova O; Abramova M; Kazalov M; Zyubko T; Artamonova T; Artamonova D; Shmakov S; Khodorkovskii M; Severinov K Nucleic Acids Res; 2020 Feb; 48(4):2026-2034. PubMed ID: 31943070 [TBL] [Abstract][Full Text] [Related]
9. Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA. Liu MS; Gong S; Yu HH; Jung K; Johnson KA; Taylor DW Nat Commun; 2020 Jul; 11(1):3576. PubMed ID: 32681021 [TBL] [Abstract][Full Text] [Related]
10. Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease. Chen W; Zhang H; Zhang Y; Wang Y; Gan J; Ji Q PLoS Biol; 2019 Oct; 17(10):e3000496. PubMed ID: 31603896 [TBL] [Abstract][Full Text] [Related]
11. Manipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency. Xie Q; Li S; Zhao D; Ye L; Li Q; Zhang X; Zhu L; Bi C Microb Cell Fact; 2020 Dec; 19(1):229. PubMed ID: 33317552 [TBL] [Abstract][Full Text] [Related]
12. Design and Assembly of CRISPR/Cas9 Lentiviral and rAAV Vectors for Targeted Genome Editing. Sandoval IM; Collier TJ; Manfredsson FP Methods Mol Biol; 2019; 1937():29-45. PubMed ID: 30706388 [TBL] [Abstract][Full Text] [Related]
13. Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels. Depardieu F; Bikard D Methods; 2020 Feb; 172():61-75. PubMed ID: 31377338 [TBL] [Abstract][Full Text] [Related]
14. Precise Regulation of Cas9-Mediated Genome Engineering by Anti-CRISPR-Based Inducible CRISPR Controllers. Jain S; Xun G; Abesteh S; Ho S; Lingamaneni M; Martin TA; Tasan I; Yang C; Zhao H ACS Synth Biol; 2021 Jun; 10(6):1320-1327. PubMed ID: 34006094 [TBL] [Abstract][Full Text] [Related]
15. Gene silencing based on RNA-guided catalytically inactive Cas9 (dCas9): a new tool for genetic engineering in Leptospira. Fernandes LGV; Guaman LP; Vasconcellos SA; Heinemann MB; Picardeau M; Nascimento ALTO Sci Rep; 2019 Feb; 9(1):1839. PubMed ID: 30755626 [TBL] [Abstract][Full Text] [Related]
16. Coordinated Actions of Cas9 HNH and RuvC Nuclease Domains Are Regulated by the Bridge Helix and the Target DNA Sequence. Babu K; Kathiresan V; Kumari P; Newsom S; Parameshwaran HP; Chen X; Liu J; Qin PZ; Rajan R Biochemistry; 2021 Dec; 60(49):3783-3800. PubMed ID: 34757726 [TBL] [Abstract][Full Text] [Related]
17. Electronic Circular Dichroism of the Cas9 Protein and gRNA:Cas9 Ribonucleoprotein Complex. Halat M; Klimek-Chodacka M; Orleanska J; Baranska M; Baranski R Int J Mol Sci; 2021 Mar; 22(6):. PubMed ID: 33805827 [TBL] [Abstract][Full Text] [Related]