162 related articles for article (PubMed ID: 33191460)
1. Amplification of EBNA-1 through a single-plasmid vector-based gene amplification system in HEK293 cells as an efficient transient gene expression system.
Park SH; Park JH; Lee JH; Lee HM; Kang YJ; Lee EJ; Shin S; Lee GM; Kim YG
Appl Microbiol Biotechnol; 2021 Jan; 105(1):67-76. PubMed ID: 33191460
[TBL] [Abstract][Full Text] [Related]
2. Co-amplification of EBNA-1 and PyLT through dhfr-mediated gene amplification for improving foreign protein production in transient gene expression in CHO cells.
Lee JH; Park JH; Park SH; Kim SH; Kim JY; Min JK; Lee GM; Kim YG
Appl Microbiol Biotechnol; 2018 Jun; 102(11):4729-4739. PubMed ID: 29654557
[TBL] [Abstract][Full Text] [Related]
3. A single-plasmid vector for transgene amplification using short hairpin RNA targeting the 3'-UTR of amplifiable dhfr.
Kang SY; Kim YG; Lee HW; Lee EG
Appl Microbiol Biotechnol; 2015 Dec; 99(23):10117-26. PubMed ID: 26245680
[TBL] [Abstract][Full Text] [Related]
4. Comprehensive characterization of dihydrofolate reductase-mediated gene amplification for the establishment of recombinant human embryonic kidney 293 cells producing monoclonal antibodies.
Lee SY; Baek M; Lee GM
Biotechnol J; 2021 May; 16(5):e2000351. PubMed ID: 33314785
[TBL] [Abstract][Full Text] [Related]
5. Short hairpin RNA targeted to dihydrofolate reductase enhances the immunoglobulin G expression in gene-amplified stable Chinese hamster ovary cells.
Wu SC; Hong WW; Liu JH
Vaccine; 2008 Sep; 26(38):4969-74. PubMed ID: 18602963
[TBL] [Abstract][Full Text] [Related]
6. Establishment of DHFR-deficient HEK293 cells for high yield of therapeutic glycoproteins.
Mensah EO; Guo XY; Gao XD; Fujita M
J Biosci Bioeng; 2019 Oct; 128(4):487-494. PubMed ID: 31031194
[TBL] [Abstract][Full Text] [Related]
7. Plasmid amplification-promoting sequences from the origin region of Chinese hamster dihydrofolate reductase gene do not promote position-independent chromosomal gene amplification.
Brinton BT; Heintz NH
Chromosoma; 1995 Nov; 104(2):143-51. PubMed ID: 8585992
[TBL] [Abstract][Full Text] [Related]
8. Improved elongation factor-1 alpha-based vectors for stable high-level expression of heterologous proteins in Chinese hamster ovary cells.
Orlova NA; Kovnir SV; Hodak JA; Vorobiev II; Gabibov AG; Skryabin KG
BMC Biotechnol; 2014 Jun; 14():56. PubMed ID: 24929670
[TBL] [Abstract][Full Text] [Related]
9. Fusion of the Dhfr/Mtx and IR/MAR gene amplification methods produces a rapid and efficient method for stable recombinant protein production.
Noguchi C; Araki Y; Miki D; Shimizu N
PLoS One; 2012; 7(12):e52990. PubMed ID: 23300841
[TBL] [Abstract][Full Text] [Related]
10. A comparison of the propensity for gene amplification between near-tetraploid and near-diploid V79 clones resistant to 150 nM methotrexate.
Hashimoto MW; Nikaido O; Kobayashi N; Chang CC; Trosko JE; Mori T
Carcinogenesis; 1996 Mar; 17(3):389-94. PubMed ID: 8631121
[TBL] [Abstract][Full Text] [Related]
11. A novel RNA silencing vector to improve antigen expression and stability in Chinese hamster ovary cells.
Hong WW; Wu SC
Vaccine; 2007 May; 25(20):4103-11. PubMed ID: 17428585
[TBL] [Abstract][Full Text] [Related]
12. Key determinants in the occurrence of clonal variation in humanized antibody expression of cho cells during dihydrofolate reductase mediated gene amplification.
Kim NS; Byun TH; Lee GM
Biotechnol Prog; 2001; 17(1):69-75. PubMed ID: 11170482
[TBL] [Abstract][Full Text] [Related]
13. [Dual gene amplification and selection system with dihydrofolate reductase and glutamine synthetase genes effectively increase the foreign gene expression].
Wang Z; Wei B; Tian S; Zhang Y; Wang X; Chu Y; Ruan L
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 2002 Mar; 16(1):59-61. PubMed ID: 11986749
[TBL] [Abstract][Full Text] [Related]
14. Construction of engineered CHO strains for high-level production of recombinant proteins.
Kito M; Itami S; Fukano Y; Yamana K; Shibui T
Appl Microbiol Biotechnol; 2002 Dec; 60(4):442-8. PubMed ID: 12466885
[TBL] [Abstract][Full Text] [Related]
15. Selection for methotrexate resistance in mammalian cells bearing a Drosophila dihydrofolate reductase transgene: Methotrexate resistance in transgenic mammalian cells.
Affleck JG; Nowickyj SM; Walker VK
Cell Biol Toxicol; 2010 Apr; 26(2):117-26. PubMed ID: 19337845
[TBL] [Abstract][Full Text] [Related]
16. Cell cycle-dependent DHFR and t-PA production in cotransfected, MTX-amplified CHO cells revealed by dual-laser flow cytometry.
Kubbies M; Stockinger H
Exp Cell Res; 1990 Jun; 188(2):267-71. PubMed ID: 2110526
[TBL] [Abstract][Full Text] [Related]
17. Non-replicating Epstein-Barr virus-based plasmids extend gene expression and can improve gene therapy in vivo.
Tu G; Kirchmaier AL; Liggitt D; Liu Y; Liu S; Yu WH; Heath TD; Thor A; Debs RJ
J Biol Chem; 2000 Sep; 275(39):30408-16. PubMed ID: 10856307
[TBL] [Abstract][Full Text] [Related]
18. Evaluating the interaction between UCOE and DHFR-linked amplification and stability of recombinant protein expression.
Betts Z; Croxford AS; Dickson AJ
Biotechnol Prog; 2015; 31(4):1014-25. PubMed ID: 25829363
[TBL] [Abstract][Full Text] [Related]
19. Generation of high-expressing cells by methotrexate amplification of destabilized dihydrofolate reductase selection marker.
Ng SK
Methods Mol Biol; 2012; 801():161-72. PubMed ID: 21987253
[TBL] [Abstract][Full Text] [Related]
20. Transfection of a nonactive site mutant murine DHFR cDNA (the tryptophan 15 mutant) into Chinese hamster ovary and mouse marrow progenitor cells imparts MTX resistance in vitro.
Banerjee D; Zhao SC; Tong Y; Steinherz J; Gritsman K; Bertino JR
Cancer Gene Ther; 1994 Sep; 1(3):181-4. PubMed ID: 7621249
[TBL] [Abstract][Full Text] [Related]
[Next] [New Search]
