197 related articles for article (PubMed ID: 33259700)
1. Concurrent transfection of randomized transgene configurations into targeted integration CHO host is an advantageous and cost-effective method for expression of complex molecules.
Dong E; Lam C; Tang D; Louie S; Yim M; Williams AJ; Sawyer W; Yip S; Carver J; AlBarakat A; Tsukuda J; Snedecor B; Misaghi S
Biotechnol J; 2021 Apr; 16(4):e2000230. PubMed ID: 33259700
[TBL] [Abstract][Full Text] [Related]
2. Genetic rearrangement during site specific integration event facilitates cell line development of a bispecific molecule.
Tevelev B; Patel H; Shields K; Wei W; Cooley C; Zhang S; Bitzas G; Duan W; Khetemenee L; Jackobek R; D'Antona A; Sievers A; King A; Tam A; Zhang Y; Sousa E; Cohen J; Wroblewska L; Marshall J; Jackson M; Scarcelli JJ
Biotechnol Prog; 2021 Jul; 37(4):e3158. PubMed ID: 33891804
[TBL] [Abstract][Full Text] [Related]
3. Targeted integration in CHO cells using CRIS-PITCh/Bxb1 recombinase-mediated cassette exchange hybrid system.
Ghanbari S; Bayat E; Azizi M; Fard-Esfahani P; Modarressi MH; Davami F
Appl Microbiol Biotechnol; 2023 Feb; 107(2-3):769-783. PubMed ID: 36536089
[TBL] [Abstract][Full Text] [Related]
4. Expressing antigen binding fragments with high titers in a targeted integration CHO host by optimizing expression vector gene copy numbers and position: A case study.
Tang D; Gunson J; Tran E; Lam C; Shen A; Snedecor B; Barnard G; Misaghi S
Biotechnol Prog; 2022 Nov; 38(6):e3290. PubMed ID: 36537257
[TBL] [Abstract][Full Text] [Related]
5. Development of a targeted integration Chinese hamster ovary host directly targeting either one or two vectors simultaneously to a single locus using the Cre/Lox recombinase-mediated cassette exchange system.
Ng D; Zhou M; Zhan D; Yip S; Ko P; Yim M; Modrusan Z; Joly J; Snedecor B; Laird MW; Shen A
Biotechnol Prog; 2021 Jul; 37(4):e3140. PubMed ID: 33666334
[TBL] [Abstract][Full Text] [Related]
6. Multicopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells.
Sergeeva D; Lee GM; Nielsen LK; Grav LM
ACS Synth Biol; 2020 Sep; 9(9):2546-2561. PubMed ID: 32835482
[TBL] [Abstract][Full Text] [Related]
7. Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell growth.
Lam C; Carver J; Ng D; Zhan D; Tang D; Kandamkalam T; Snedecor B; Barnard G; Shen A; Misaghi S
Biotechnol Prog; 2023; 39(3):e3337. PubMed ID: 36878664
[TBL] [Abstract][Full Text] [Related]
8. Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35.
Zhao M; Wang J; Luo M; Luo H; Zhao M; Han L; Zhang M; Yang H; Xie Y; Jiang H; Feng L; Lu H; Zhu J
Appl Microbiol Biotechnol; 2018 Jul; 102(14):6105-6117. PubMed ID: 29789882
[TBL] [Abstract][Full Text] [Related]
9. Controlling Ratios of Plasmid-Based Double Cut Donor and CRISPR/Cas9 Components to Enhance Targeted Integration of Transgenes in Chinese Hamster Ovary Cells.
Shin SW; Kim D; Lee JS
Int J Mol Sci; 2021 Feb; 22(5):. PubMed ID: 33673701
[TBL] [Abstract][Full Text] [Related]
10. The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability.
Matasci M; Baldi L; Hacker DL; Wurm FM
Biotechnol Bioeng; 2011 Sep; 108(9):2141-50. PubMed ID: 21495018
[TBL] [Abstract][Full Text] [Related]
11. Fast identification of reliable hosts for targeted cell line development from a limited-genome screening using combined φC31 integrase and CRE-Lox technologies.
Crawford Y; Zhou M; Hu Z; Joly J; Snedecor B; Shen A; Gao A
Biotechnol Prog; 2013; 29(5):1307-15. PubMed ID: 23926028
[TBL] [Abstract][Full Text] [Related]
12. Gene copy number, gene configuration and LC/HC mRNA ratio impact on antibody productivity and product quality in targeted integration CHO cell lines.
Lee Z; Wan J; Shen A; Barnard G
Biotechnol Prog; 2024; 40(3):e3433. PubMed ID: 38321634
[TBL] [Abstract][Full Text] [Related]
13. Generation of a host cell line containing a MAR-rich landing pad for site-specific integration and expression of transgenes.
Oliviero C; Hinz SC; Bogen JP; Kornmann H; Hock B; Kolmar H; Hagens G
Biotechnol Prog; 2022 Jul; 38(4):e3254. PubMed ID: 35396920
[TBL] [Abstract][Full Text] [Related]
14. Accumulative scFv-Fc antibody gene integration into the hprt chromosomal locus of Chinese hamster ovary cells.
Wang X; Kawabe Y; Kato R; Hada T; Ito A; Yamana Y; Kondo M; Kamihira M
J Biosci Bioeng; 2017 Nov; 124(5):583-590. PubMed ID: 28662917
[TBL] [Abstract][Full Text] [Related]
15. Structural analysis of random transgene integration in CHO manufacturing cell lines by targeted sequencing.
Stadermann A; Gamer M; Fieder J; Lindner B; Fehrmann S; Schmidt M; Schulz P; Gorr IH
Biotechnol Bioeng; 2022 Mar; 119(3):868-880. PubMed ID: 34935125
[TBL] [Abstract][Full Text] [Related]
16. Nanopore Cas9-targeted sequencing enables accurate and simultaneous identification of transgene integration sites, their structure and epigenetic status in recombinant Chinese hamster ovary cells.
Leitner K; Motheramgari K; Borth N; Marx N
Biotechnol Bioeng; 2023 Sep; 120(9):2403-2418. PubMed ID: 36938677
[TBL] [Abstract][Full Text] [Related]
17. Improved recombinant antibody production by CHO cells using a production enhancer DNA element with repeated transgene integration at a predetermined chromosomal site.
Kawabe Y; Inao T; Komatsu S; Huang G; Ito A; Omasa T; Kamihira M
J Biosci Bioeng; 2017 Mar; 123(3):390-397. PubMed ID: 27856232
[TBL] [Abstract][Full Text] [Related]
18. Utilizing targeted integration CHO pools to potentially accelerate the GMP manufacturing of monoclonal and bispecific antibodies.
Barnard GC; Zhou M; Shen A; Yuk IH; Laird MW
Biotechnol Prog; 2024; 40(1):e3399. PubMed ID: 37874920
[TBL] [Abstract][Full Text] [Related]
19. Cre-Mediated Transgene Integration in Chinese Hamster Ovary Cells Using Minicircle DNA Vectors.
Wang X; Kawabe Y; Hada T; Ito A; Kamihira M
Biotechnol J; 2018 Jul; 13(7):e1800063. PubMed ID: 29701326
[TBL] [Abstract][Full Text] [Related]
20. Generation of stable cell lines by site-specific integration of transgenes into engineered Chinese hamster ovary strains using an FLP-FRT system.
Zhou H; Liu ZG; Sun ZW; Huang Y; Yu WY
J Biotechnol; 2010 May; 147(2):122-9. PubMed ID: 20371256
[TBL] [Abstract][Full Text] [Related]
[Next] [New Search]
