These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
163 related articles for article (PubMed ID: 34001951)
1. A high-efficiency method for site-directed mutagenesis of large plasmids based on large DNA fragment amplification and recombinational ligation. Zhang K; Yin X; Shi K; Zhang S; Wang J; Zhao S; Deng H; Zhang C; Wu Z; Li Y; Zhou X; Deng W Sci Rep; 2021 May; 11(1):10454. PubMed ID: 34001951 [TBL] [Abstract][Full Text] [Related]
2. A simple and economical site-directed mutagenesis method for large plasmids by direct transformation of two overlapping PCR fragments. Yang Z; Chen Z; Zhang Y Biotechniques; 2022 Nov; 73(5):239-245. PubMed ID: 36398840 [TBL] [Abstract][Full Text] [Related]
4. Efficient cloning of linear DNA inserts (ECOLI) into plasmids using site-directed mutagenesis. Paclikova P; Harnos J Sci Rep; 2024 Sep; 14(1):21591. PubMed ID: 39284917 [TBL] [Abstract][Full Text] [Related]
5. Seamless Ligation Cloning Extract (SLiCE) Method Using Cell Lysates from Laboratory Escherichia coli Strains and its Application to SLiP Site-Directed Mutagenesis. Motohashi K Methods Mol Biol; 2017; 1498():349-357. PubMed ID: 27709587 [TBL] [Abstract][Full Text] [Related]
6. [Site-directed mutagenesis of long gene by partial amplification combining with double fragments ligation]. Xiao J; Ma M; Liang M; He R; Chen H Sheng Wu Gong Cheng Xue Bao; 2020 Jun; 36(6):1232-1240. PubMed ID: 32597073 [TBL] [Abstract][Full Text] [Related]
7. Improvement of PCR reaction conditions for site-directed mutagenesis of big plasmids. Munteanu B; Braun M; Boonrod K J Zhejiang Univ Sci B; 2012 Apr; 13(4):244-7. PubMed ID: 22467364 [TBL] [Abstract][Full Text] [Related]
8. Highly efficient one-step PCR-based mutagenesis technique for large plasmids using high-fidelity DNA polymerase. Liu H; Ye R; Wang YY Genet Mol Res; 2015 Apr; 14(2):3466-73. PubMed ID: 25966113 [TBL] [Abstract][Full Text] [Related]
9. REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering. Trehan A; Kiełbus M; Czapinski J; Stepulak A; Huhtaniemi I; Rivero-Müller A Sci Rep; 2016 Jan; 6():19121. PubMed ID: 26750263 [TBL] [Abstract][Full Text] [Related]
10. PTO-QuickStep: A Fast and Efficient Method for Cloning Random Mutagenesis Libraries. Jajesniak P; Tee KL; Wong TS Int J Mol Sci; 2019 Aug; 20(16):. PubMed ID: 31405219 [TBL] [Abstract][Full Text] [Related]
11. A highly efficient procedure for site-specific mutagenesis of full-length plasmids using Vent DNA polymerase. Byrappa S; Gavin DK; Gupta KC Genome Res; 1995 Nov; 5(4):404-7. PubMed ID: 8750200 [TBL] [Abstract][Full Text] [Related]
12. Efficient method for site-directed mutagenesis in large plasmids without subcloning. Hallak LK; Berger K; Kaspar R; Kwilas AR; Montanaro F; Peeples ME PLoS One; 2017; 12(6):e0177788. PubMed ID: 28575024 [TBL] [Abstract][Full Text] [Related]
13. A method for multi-site-directed mutagenesis based on homologous recombination. Liang X; Peng L; Li K; Peterson T; Katzen F Anal Biochem; 2012 Aug; 427(1):99-101. PubMed ID: 22579595 [TBL] [Abstract][Full Text] [Related]
14. Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids. Jia H; Couto-Rodriguez R; Johnson S; Medina S; Novillo B; Huynh P; Kim M; Cooper C; Michalik M; Siew B; Turesson E; Maupin-Furlow JA N Biotechnol; 2022 Dec; 72():22-28. PubMed ID: 36007808 [TBL] [Abstract][Full Text] [Related]
15. Site-directed mutagenesis of large (13-kb) plasmids in a single-PCR procedure. Wang J; Wilkinson MF Biotechniques; 2000 Nov; 29(5):976-8. PubMed ID: 11084857 [No Abstract] [Full Text] [Related]
16. Multichange isothermal mutagenesis: a new strategy for multiple site-directed mutations in plasmid DNA. Mitchell LA; Cai Y; Taylor M; Noronha AM; Chuang J; Dai L; Boeke JD ACS Synth Biol; 2013 Aug; 2(8):473-7. PubMed ID: 23654272 [TBL] [Abstract][Full Text] [Related]
17. [Using multiple-fragment amplification combined with Gibson assembly to clone genes with site-directed mutations]. Cheng Y; Li G; Liu J; Chen W; Chen H Sheng Wu Gong Cheng Xue Bao; 2022 Mar; 38(3):1218-1226. PubMed ID: 35355487 [TBL] [Abstract][Full Text] [Related]
18. Single-round QuikChange PCR for engineering multiple site-directed mutations in plasmid DNA. Li Y; Pinones M; Breeland A; Jiang P Anal Biochem; 2024 Nov; 694():115621. PubMed ID: 39019205 [TBL] [Abstract][Full Text] [Related]
19. Efficient strategy for introducing large and multiple changes in plasmid DNA. Zeng F; Zhang S; Hao Z; Duan S; Meng Y; Li P; Dong J; Lin Y Sci Rep; 2018 Jan; 8(1):1714. PubMed ID: 29379085 [TBL] [Abstract][Full Text] [Related]
20. Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Ma Y; Zhang B; Wang M; Ou Y; Wang J; Li S Biomed Res Int; 2016; 2016():2906484. PubMed ID: 27981047 [TBL] [Abstract][Full Text] [Related] [Next] [New Search]