These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


BIOMARKERS

Molecular Biopsy of Human Tumors

- a resource for Precision Medicine *

115 related articles for article (PubMed ID: 8346048)

  • 1. DISEC-TRISEC: di- and trinucleotide-sticky-end cloning of PCR-amplified DNA.
    Dietmaier W; Fabry S; Schmitt R
    Nucleic Acids Res; 1993 Jul; 21(15):3603-4. PubMed ID: 8346048
    [No Abstract]   [Full Text] [Related]  

  • 2. An optimized recipe for cloning of the polymerase chain reaction-amplified DNA inserts into plasmid vectors.
    Topcu Z
    Acta Biochim Pol; 2000; 47(3):841-6. PubMed ID: 11310983
    [TBL] [Abstract][Full Text] [Related]  

  • 3. Functional cloning vectors for use in directional cDNA cloning using cohesive ends produced with T4 DNA polymerase.
    Kuijper JL; Wiren KM; Mathies LD; Gray CL; Hagen FS
    Gene; 1992 Mar; 112(2):147-55. PubMed ID: 1532564
    [TBL] [Abstract][Full Text] [Related]  

  • 4. Polishing with T4 or Pfu polymerase increases the efficiency of cloning of PCR fragments.
    Costa GL; Weiner MP
    Nucleic Acids Res; 1994 Jun; 22(12):2423. PubMed ID: 8036174
    [No Abstract]   [Full Text] [Related]  

  • 5. Limited T4 exonuclease activity and partial fill-in expand insertion site options for PCR subcloning.
    Green N; Vu S; Farahmand S; Sharp SB
    Biotechniques; 1999 Nov; 27(5):914-6. PubMed ID: 10572636
    [No Abstract]   [Full Text] [Related]  

  • 6. Preparation of long sticky ends for universal ligation-independent cloning: sequential T4 DNA polymerase treatments.
    Tachibana A; Tohiguchi K; Ueno T; Setogawa Y; Harada A; Tanabe T
    J Biosci Bioeng; 2009 Jun; 107(6):668-9. PubMed ID: 19447347
    [TBL] [Abstract][Full Text] [Related]  

  • 7. Ligation-independent cloning of PCR products (LIC-PCR).
    Aslanidis C; de Jong PJ
    Nucleic Acids Res; 1990 Oct; 18(20):6069-74. PubMed ID: 2235490
    [TBL] [Abstract][Full Text] [Related]  

  • 8. General method for site-directed mutagenesis and cloning of synthetic single-stranded DNA in circular vectors.
    Drutsa VL; Kaberdin VR
    Nucleic Acids Symp Ser; 1991; (24):300. PubMed ID: 1841357
    [No Abstract]   [Full Text] [Related]  

  • 9. Using T4 DNA polymerase to generate clonable PCR products.
    Wang K
    Methods Mol Biol; 2002; 192():121-4. PubMed ID: 12494644
    [No Abstract]   [Full Text] [Related]  

  • 10. Use of M13 ping-pong vectors and T4 DNA polymerase in oligodeoxynucleotide-directed mutagenesis.
    Waye MM
    Methods Enzymol; 1993; 217():258-70. PubMed ID: 8386290
    [No Abstract]   [Full Text] [Related]  

  • 11. General method for direct cloning of DNA fragments generated by the polymerase chain reaction.
    Kovalic D; Kwak JH; Weisblum B
    Nucleic Acids Res; 1991 Aug; 19(16):4560. PubMed ID: 1886782
    [No Abstract]   [Full Text] [Related]  

  • 12. Directional enrichment of directly cloned PCR products.
    Horn D
    Biotechniques; 2005 Jul; 39(1):40, 42, 44, 46. PubMed ID: 16060367
    [No Abstract]   [Full Text] [Related]  

  • 13. Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase.
    Abdus Sattar AK; Lin TC; Jones C; Konigsberg WH
    Biochemistry; 1996 Dec; 35(51):16621-9. PubMed ID: 8987997
    [TBL] [Abstract][Full Text] [Related]  

  • 14. One-step sequence- and ligation-independent cloning as a rapid and versatile cloning method for functional genomics studies.
    Jeong JY; Yim HS; Ryu JY; Lee HS; Lee JH; Seen DS; Kang SG
    Appl Environ Microbiol; 2012 Aug; 78(15):5440-3. PubMed ID: 22610439
    [TBL] [Abstract][Full Text] [Related]  

  • 15. Quick and clean cloning.
    Thieme F; Marillonnet S
    Methods Mol Biol; 2014; 1116():37-48. PubMed ID: 24395355
    [TBL] [Abstract][Full Text] [Related]  

  • 16. A modified plasmid vector pCMV-3Tag-LIC for rapid, reliable, ligation-independent cloning of polymerase chain reaction products.
    Du R; Li S; Zhang X
    Anal Biochem; 2011 Jan; 408(2):357-9. PubMed ID: 20816743
    [TBL] [Abstract][Full Text] [Related]  

  • 17. PCR cloning using T-vectors.
    Trower MK; Elgar GS
    Methods Mol Biol; 1994; 31():19-33. PubMed ID: 7921018
    [No Abstract]   [Full Text] [Related]  

  • 18. Generation of plasmid vectors expressing FLAG-tagged proteins under the regulation of human elongation factor-1α promoter using Gibson assembly.
    Grozdanov PN; MacDonald CC
    J Vis Exp; 2015 Feb; (96):. PubMed ID: 25742071
    [TBL] [Abstract][Full Text] [Related]  

  • 19. Plasmid construction by SLIC or sequence and ligation-independent cloning.
    Hill RE; Eaton-Rye JJ
    Methods Mol Biol; 2014; 1116():25-36. PubMed ID: 24395354
    [TBL] [Abstract][Full Text] [Related]  

  • 20. Cloning Polymerase Chain Reaction (PCR) Products: Making T Vectors.
    Green MR; Sambrook J
    Cold Spring Harb Protoc; 2021 Jun; 2021(6):. PubMed ID: 34074738
    [TBL] [Abstract][Full Text] [Related]  

    [Next]    [New Search]
    of 6.