118 related articles for article (PubMed ID: 8422991)
1. Increased antibody expression from Escherichia coli through wobble-base library mutagenesis by enzymatic inverse PCR.
Stemmer WP; Morris SK; Kautzer CR; Wilson BS
Gene; 1993 Jan; 123(1):1-7. PubMed ID: 8422991
[TBL] [Abstract][Full Text] [Related]
2. Selection of an active single chain Fv antibody from a protein linker library prepared by enzymatic inverse PCR.
Stemmer WP; Morris SK; Wilson BS
Biotechniques; 1993 Feb; 14(2):256-65. PubMed ID: 8431292
[TBL] [Abstract][Full Text] [Related]
3. Selection of antibody single-chain variable fragments with improved carbohydrate binding by phage display.
Deng SJ; MacKenzie CR; Sadowska J; Michniewicz J; Young NM; Bundle DR; Narang SA
J Biol Chem; 1994 Apr; 269(13):9533-8. PubMed ID: 8144539
[TBL] [Abstract][Full Text] [Related]
4. Single-chain anti-DNA FV.
Polymenis M; Stollar BD
Methods Mol Biol; 1995; 51():265-79. PubMed ID: 7581703
[No Abstract] [Full Text] [Related]
5. A rapid method of cloning functional variable-region antibody genes in Escherichia coli as single-chain immunotoxins.
Chaudhary VK; Batra JK; Gallo MG; Willingham MC; FitzGerald DJ; Pastan I
Proc Natl Acad Sci U S A; 1990 Feb; 87(3):1066-70. PubMed ID: 2105495
[TBL] [Abstract][Full Text] [Related]
6. Enzymatic inverse PCR: a restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis.
Stemmer WP; Morris SK
Biotechniques; 1992 Aug; 13(2):214-20. PubMed ID: 1327007
[TBL] [Abstract][Full Text] [Related]
7. Rapid restriction enzyme-free cloning of PCR products: a high-throughput method applicable for library construction.
Chaudhary VK; Shrivastava N; Verma V; Das S; Kaur C; Grover P; Gupta A
PLoS One; 2014; 9(10):e111538. PubMed ID: 25360695
[TBL] [Abstract][Full Text] [Related]
8. Easy preparation of a large-size random gene mutagenesis library in Escherichia coli.
You C; Percival Zhang YH
Anal Biochem; 2012 Sep; 428(1):7-12. PubMed ID: 22659340
[TBL] [Abstract][Full Text] [Related]
9. Synthetic antibody gene libraries for in vitro affinity maturation.
Deng SJ; MacKenzie CR; Narang SA
Methods Mol Biol; 1995; 51():329-42. PubMed ID: 7581707
[No Abstract] [Full Text] [Related]
10. MEGAWHOP cloning: a method of creating random mutagenesis libraries via megaprimer PCR of whole plasmids.
Miyazaki K
Methods Enzymol; 2011; 498():399-406. PubMed ID: 21601687
[TBL] [Abstract][Full Text] [Related]
11. Engineering strand-specific DNA nicking enzymes from the type IIS restriction endonucleases BsaI, BsmBI, and BsmAI.
Zhu Z; Samuelson JC; Zhou J; Dore A; Xu SY
J Mol Biol; 2004 Mar; 337(3):573-83. PubMed ID: 15019778
[TBL] [Abstract][Full Text] [Related]
12. A revised strategy for cloning antibody gene fragments in bacteria.
Posner B; Lee I; Itoh T; Pyati J; Graff R; Thorton GB; La Polla R; Benkovic SJ
Gene; 1993 Jun; 128(1):111-7. PubMed ID: 8508951
[TBL] [Abstract][Full Text] [Related]
13. Suppression of replication-deficient mutants of IncFII plasmid NR1 can occur by two different mechanisms that increase expression of the repA1 gene.
Wu R; Wang X; Womble DD; Rownd RH
J Bacteriol; 1993 May; 175(10):3161-73. PubMed ID: 8491730
[TBL] [Abstract][Full Text] [Related]
14. Isolated VH4 heavy chain variable regions bind DNA characterization of a recombinant antibody heavy chain library derived from patient(s) with active SLE.
Kieber-Emmons T; von Feldt JM; Godillot AP; McCallus D; Srikantan V; Weiner DB; Williams WV
Lupus; 1994 Oct; 3(5):379-92. PubMed ID: 7841991
[TBL] [Abstract][Full Text] [Related]
15. Increased protein expression through improved ribosome-binding sites obtained by library mutagenesis.
Wilson BS; Kautzer CR; Antelman DE
Biotechniques; 1994 Nov; 17(5):944-53. PubMed ID: 7840977
[TBL] [Abstract][Full Text] [Related]
16. Expression of a heterodimeric Fab antibody protein in one cloning step.
Mullinax RL; Gross EA; Hay BN; Amberg JR; Kubitz MM; Sorge JA
Biotechniques; 1992 Jun; 12(6):864-9. PubMed ID: 1642894
[TBL] [Abstract][Full Text] [Related]
17. The influence of messenger RNA secondary structure on expression of an immunoglobulin heavy chain in Escherichia coli.
Wood CR; Boss MA; Patel TP; Emtage JS
Nucleic Acids Res; 1984 May; 12(9):3937-50. PubMed ID: 6328446
[TBL] [Abstract][Full Text] [Related]
18. Phage-display libraries of murine and human antibody Fab fragments.
Engberg J; Andersen PS; Nielsen LK; Dziegiel M; Johansen LK; Albrechtsen B
Methods Mol Biol; 1995; 51():355-76. PubMed ID: 7581709
[No Abstract] [Full Text] [Related]
19. Comparison of the Fv fragments of different phosphorylcholine binding antibodies expressed in Escherichia coli.
Plückthun A; Pfitzinger I
Ann N Y Acad Sci; 1991 Dec; 646():115-24. PubMed ID: 1809182
[TBL] [Abstract][Full Text] [Related]
20. Secretion of recombinant archeal lipase mediated by SVP2 signal peptide in Escherichia coli and its optimization by response surface methodology.
Pournejati R; Karbalaei-Heidari HR; Budisa N
Protein Expr Purif; 2014 Sep; 101():84-90. PubMed ID: 24907409
[TBL] [Abstract][Full Text] [Related]
[Next] [New Search]