125 related articles for article (PubMed ID: 8796322)
1. Random mutagenesis of the weak calcium binding site in subtilisin Carlsberg and screening for thermostability by temperature-gradient gel electrophoresis.
Sättler A; Kanka S; Schrörs W; Riesner D
Adv Exp Med Biol; 1996; 379():171-82. PubMed ID: 8796322
[TBL] [Abstract][Full Text] [Related]
2. Thermostable variants of subtilisin selected by temperature-gradient gel electrophoresis.
Sättler A; Kanka S; Maurer KH; Riesner D
Electrophoresis; 1996 Apr; 17(4):784-92. PubMed ID: 8738345
[TBL] [Abstract][Full Text] [Related]
3. Temperature-gradient gel electrophoresis for analysis and screening of thermostable proteases.
Sättler A; Riesner D
Electrophoresis; 1993 Aug; 14(8):782-8. PubMed ID: 8404822
[TBL] [Abstract][Full Text] [Related]
4. Effect of the weak Ca(2+)-binding site of subtilisin J by site-directed mutagenesis on heat stability.
Jang JS; Bae KH; Byun SM
Biochem Biophys Res Commun; 1992 Oct; 188(1):184-9. PubMed ID: 1358066
[TBL] [Abstract][Full Text] [Related]
5. Directed evolution study of temperature adaptation in a psychrophilic enzyme.
Miyazaki K; Wintrode PL; Grayling RA; Rubingh DN; Arnold FH
J Mol Biol; 2000 Apr; 297(4):1015-26. PubMed ID: 10736234
[TBL] [Abstract][Full Text] [Related]
6. Subtilisin from psychrophilic antarctic bacteria: characterization and site-directed mutagenesis of residues possibly involved in the adaptation to cold.
Narinx E; Baise E; Gerday C
Protein Eng; 1997 Nov; 10(11):1271-9. PubMed ID: 9514115
[TBL] [Abstract][Full Text] [Related]
7. Calcium-mediated thermostability in the subtilisin superfamily: the crystal structure of Bacillus Ak.1 protease at 1.8 A resolution.
Smith CA; Toogood HS; Baker HM; Daniel RM; Baker EN
J Mol Biol; 1999 Dec; 294(4):1027-40. PubMed ID: 10588904
[TBL] [Abstract][Full Text] [Related]
8. Enhanced stability of subtilisin by three point mutations.
Narhi LO; Stabinsky Y; Levitt M; Miller L; Sachdev R; Finley S; Park S; Kolvenbach C; Arakawa T; Zukowski M
Biotechnol Appl Biochem; 1991 Feb; 13(1):12-24. PubMed ID: 2054102
[TBL] [Abstract][Full Text] [Related]
9. Formation of the high-affinity calcium binding site in pro-subtilisin E with the insertion sequence IS1 of pro-Tk-subtilisin.
Uehara R; Angkawidjaja C; Koga Y; Kanaya S
Biochemistry; 2013 Dec; 52(50):9080-8. PubMed ID: 24279884
[TBL] [Abstract][Full Text] [Related]
10. Protein engineering of subtilisins to improve stability in detergent formulations.
von der Osten C; Branner S; Hastrup S; Hedegaard L; Rasmussen MD; Bisgård-Frantzen H; Carlsen S; Mikkelsen JM
J Biotechnol; 1993 Mar; 28(1):55-68. PubMed ID: 7763525
[TBL] [Abstract][Full Text] [Related]
11. Engineering thermostability in subtilisin BPN' by in vitro mutagenesis.
Rollence ML; Filpula D; Pantoliano MW; Bryan PN
Crit Rev Biotechnol; 1988; 8(3):217-24. PubMed ID: 3145814
[TBL] [Abstract][Full Text] [Related]
12. Molecular cloning of a subtilisin J gene from Bacillus stearothermophilus and its expression in Bacillus subtilis.
Jang JS; Kang DO; Chun MJ; Byun SM
Biochem Biophys Res Commun; 1992 Apr; 184(1):277-82. PubMed ID: 1567435
[TBL] [Abstract][Full Text] [Related]
13. Incorporation of a stabilizing Ca(2+)-binding loop into subtilisin BPN'.
Braxton S; Wells JA
Biochemistry; 1992 Sep; 31(34):7796-801. PubMed ID: 1510966
[TBL] [Abstract][Full Text] [Related]
14. Improvement of a useful enzyme (subtilisin BPN') by an experimental evolution system.
Tange T; Taguchi S; Kojima S; Miura K; Momose H
Appl Microbiol Biotechnol; 1994 Apr; 41(2):239-44. PubMed ID: 7764834
[TBL] [Abstract][Full Text] [Related]
15. The roles of surface loop insertions and disulfide bond in the stabilization of thermophilic WF146 protease.
Bian Y; Liang X; Fang N; Tang XF; Tang B; Shen P; Peng Z
FEBS Lett; 2006 Oct; 580(25):6007-14. PubMed ID: 17052711
[TBL] [Abstract][Full Text] [Related]
16. Gly or Ala substitutions for Pro(210)Thr(211)Asn(212) at the β8-β9 turn of subtilisin Carlsberg increase the catalytic rate and decrease thermostability.
Fuchita N; Arita S; Ikuta J; Miura M; Shimomura K; Motoshima H; Watanabe K
Biochim Biophys Acta; 2012 Apr; 1824(4):620-6. PubMed ID: 22326746
[TBL] [Abstract][Full Text] [Related]
17. Preprosubtilisin Carlsberg processing and secretion is blocked after deletion of amino acids 97-101 in the mature part of the enzyme.
Schülein R; Kreft J; Gonski S; Goebel W
Mol Gen Genet; 1991 May; 227(1):137-43. PubMed ID: 1904534
[TBL] [Abstract][Full Text] [Related]
18. Crystal structure of subtilisin DY, a random mutant of subtilisin Carlsberg.
Eschenburg S; Genov N; Peters K; Fittkau S; Stoeva S; Wilson KS; Betzel C
Eur J Biochem; 1998 Oct; 257(2):309-18. PubMed ID: 9826175
[TBL] [Abstract][Full Text] [Related]
19. Isolation, characterization and structure of subtilisin from a thermostable Bacillus subtilis isolate.
Kamal M; Höög JO; Kaiser R; Shafqat J; Razzaki T; Zaidi ZH; Jörnvall H
FEBS Lett; 1995 Nov; 374(3):363-6. PubMed ID: 7589571
[TBL] [Abstract][Full Text] [Related]
20. Enhanced thermostability of the single-Cys mutant subtilisin E under oxidizing conditions.
Takagi H; Hirai K; Wada M; Nakamori S
J Biochem; 2000 Oct; 128(4):585-9. PubMed ID: 11011140
[TBL] [Abstract][Full Text] [Related]
[Next] [New Search]