These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
2. Identification by mutagenesis of arginines in the substrate binding site of the porcine NADP-dependent isocitrate dehydrogenase. Soundar S; Danek BL; Colman RF J Biol Chem; 2000 Feb; 275(8):5606-12. PubMed ID: 10681542 [TBL] [Abstract][Full Text] [Related]
3. Evaluation by site-directed mutagenesis of aspartic acid residues in the metal site of pig heart NADP-dependent isocitrate dehydrogenase. Grodsky NB; Soundar S; Colman RF Biochemistry; 2000 Mar; 39(9):2193-200. PubMed ID: 10694384 [TBL] [Abstract][Full Text] [Related]
4. Expression and mutagenesis of mammalian cytosolic NADP+-specific isocitrate dehydrogenase. Jennings GT; Minard KI; McAlister-Henn L Biochemistry; 1997 Nov; 36(44):13743-7. PubMed ID: 9354646 [TBL] [Abstract][Full Text] [Related]
5. Evaluation by mutagenesis of the roles of His309, His315, and His319 in the coenzyme site of pig heart NADP-dependent isocitrate dehydrogenase. Huang YC; Colman RF Biochemistry; 2002 Apr; 41(17):5637-43. PubMed ID: 11969425 [TBL] [Abstract][Full Text] [Related]
6. Characterization of purified recombinant Bet v 1 with authentic N-terminus, cloned in fusion with maltose-binding protein. Spangfort MD; Ipsen H; Sparholt SH; Aasmul-Olsen S; Larsen MR; Mørtz E; Roepstorff P; Larsen JN Protein Expr Purif; 1996 Nov; 8(3):365-73. PubMed ID: 8936599 [TBL] [Abstract][Full Text] [Related]
7. Expression of recombinant human phenylalanine hydroxylase as fusion protein in Escherichia coli circumvents proteolytic degradation by host cell proteases. Isolation and characterization of the wild-type enzyme. Martinez A; Knappskog PM; Olafsdottir S; Døskeland AP; Eiken HG; Svebak RM; Bozzini M; Apold J; Flatmark T Biochem J; 1995 Mar; 306 ( Pt 2)(Pt 2):589-97. PubMed ID: 7887915 [TBL] [Abstract][Full Text] [Related]
8. High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system. Pryor KD; Leiting B Protein Expr Purif; 1997 Aug; 10(3):309-19. PubMed ID: 9268677 [TBL] [Abstract][Full Text] [Related]
9. Implication by site-directed mutagenesis of Arg314 and Tyr316 in the coenzyme site of pig mitochondrial NADP-dependent isocitrate dehydrogenase. Lee P; Colman RF Arch Biochem Biophys; 2002 May; 401(1):81-90. PubMed ID: 12054490 [TBL] [Abstract][Full Text] [Related]
10. Crystal structure of porcine mitochondrial NADP+-dependent isocitrate dehydrogenase complexed with Mn2+ and isocitrate. Insights into the enzyme mechanism. Ceccarelli C; Grodsky NB; Ariyaratne N; Colman RF; Bahnson BJ J Biol Chem; 2002 Nov; 277(45):43454-62. PubMed ID: 12207025 [TBL] [Abstract][Full Text] [Related]
11. Purification and characterization of decorin core protein expressed in Escherichia coli as a maltose-binding protein fusion. Hering TM; Kollar J; Huynh TD; Varelas JB Anal Biochem; 1996 Aug; 240(1):98-108. PubMed ID: 8811884 [TBL] [Abstract][Full Text] [Related]
13. Overproduction and purification of Lon protease from Escherichia coli using a maltose-binding protein fusion system. Sonezaki S; Kondo A; Oba T; Ishii Y; Kato Y; Nakayama H Appl Microbiol Biotechnol; 1994 Nov; 42(2-3):313-8. PubMed ID: 7765772 [TBL] [Abstract][Full Text] [Related]
14. Thr373, Asp375, and Lys260 are in the coenzyme site of porcine NADP-dependent isocitrate dehydrogenase. Lee P; Colman RF Arch Biochem Biophys; 2006 Jun; 450(2):183-90. PubMed ID: 16712772 [TBL] [Abstract][Full Text] [Related]
15. The subcellular location of isozymes of NADP-isocitrate dehydrogenase in tissues from pig, ox and rat. Plaut GW; Cook M; Aogaichi T Biochim Biophys Acta; 1983 Oct; 760(2):300-8. PubMed ID: 6414522 [TBL] [Abstract][Full Text] [Related]
16. Expression in Escherichia coli and purification of human eosinophil-derived neurotoxin with ribonuclease activity. Sun L; Li MS; Fisher LM; Spry CJ Protein Expr Purif; 1995 Oct; 6(5):685-92. PubMed ID: 8535163 [TBL] [Abstract][Full Text] [Related]
17. Autoprocessing of the HIV-1 protease using purified wild-type and mutated fusion proteins expressed at high levels in Escherichia coli. Louis JM; McDonald RA; Nashed NT; Wondrak EM; Jerina DM; Oroszlan S; Mora PT Eur J Biochem; 1991 Jul; 199(2):361-9. PubMed ID: 2070793 [TBL] [Abstract][Full Text] [Related]
18. Ser95, Asn97, and Thr78 are important for the catalytic function of porcine NADP-dependent isocitrate dehydrogenase. Kim TK; Colman RF Protein Sci; 2005 Jan; 14(1):140-7. PubMed ID: 15576556 [TBL] [Abstract][Full Text] [Related]
19. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. di Guan C; Li P; Riggs PD; Inouye H Gene; 1988 Jul; 67(1):21-30. PubMed ID: 2843437 [TBL] [Abstract][Full Text] [Related]
20. Purification and activity of a wheat 9-kDa lipid transfer protein expressed in Escherichia coli as a fusion with the maltose binding protein. Dieryck W; Lullien-Pellerin V; Marion D; Joudrier P; Gautier MF Protein Expr Purif; 1995 Oct; 6(5):597-603. PubMed ID: 8535151 [TBL] [Abstract][Full Text] [Related] [Next] [New Search]