122 related articles for article (PubMed ID: 9681871)
1. Protein engineering of Aspergillus awamori glucoamylase to increase its pH optimum.
Fang TY; Ford C
Protein Eng; 1998 May; 11(5):383-8. PubMed ID: 9681871
[TBL] [Abstract][Full Text] [Related]
2. Mutations to alter Aspergillus awamori glucoamylase selectivity. I. Tyr48Phe49-->Trp, Tyr116-->Trp, Tyr175-->Phe, Arg241-->Lys, Ser411-->Ala and Ser411-->Gly.
Fang TY; Coutinho PM; Reilly PJ; Ford C
Protein Eng; 1998 Feb; 11(2):119-26. PubMed ID: 9605546
[TBL] [Abstract][Full Text] [Related]
3. Catalytic mechanism of glucoamylase probed by mutagenesis in conjunction with hydrolysis of alpha-D-glucopyranosyl fluoride and maltooligosaccharides.
Sierks MR; Svensson B
Biochemistry; 1996 Feb; 35(6):1865-71. PubMed ID: 8639668
[TBL] [Abstract][Full Text] [Related]
4. Enzymatic properties of the cysteinesulfinic acid derivative of the catalytic-base mutant Glu400-->Cys of glucoamylase from Aspergillus awamori.
Fierobe HP; Clarke AJ; Tull D; Svensson B
Biochemistry; 1998 Mar; 37(11):3753-9. PubMed ID: 9521694
[TBL] [Abstract][Full Text] [Related]
5. Mutations to alter Aspergillus awamori glucoamylase selectivity. III. Asn20-->Cys/Ala27-->Cys, Ala27-->Pro, Ser30-->Pro, Lys108-->Arg, Lys108-->Met, Gly137-->Ala, 311-314 Loop, Tyr312-->Trp and Ser436-->Pro.
Liu HL; Coutinho PM; Ford C; Reilly PJ
Protein Eng; 1998 May; 11(5):389-98. PubMed ID: 9681872
[TBL] [Abstract][Full Text] [Related]
6. Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of Asp176, Glu179 and Glu180 in the enzyme from Aspergillus awamori.
Sierks MR; Ford C; Reilly PJ; Svensson B
Protein Eng; 1990 Jan; 3(3):193-8. PubMed ID: 1970434
[TBL] [Abstract][Full Text] [Related]
7. Mutations to alter Aspergillus awamori glucoamylase selectivity. II. Mutation of residues 119 and 121.
Fang TY; Honzatko RB; Reilly PJ; Ford C
Protein Eng; 1998 Feb; 11(2):127-33. PubMed ID: 9605547
[TBL] [Abstract][Full Text] [Related]
8. Functional roles of the invariant aspartic acid 55, tyrosine 306, and aspartic acid 309 in glucoamylase from Aspergillus awamori studied by mutagenesis.
Sierks MR; Svensson B
Biochemistry; 1993 Feb; 32(4):1113-7. PubMed ID: 8424940
[TBL] [Abstract][Full Text] [Related]
9. Restoration of catalytic activity beyond wild-type level in glucoamylase from Aspergillus awamori by oxidation of the Glu400-->Cys catalytic-base mutant to cysteinesulfinic acid.
Fierobe HP; Mirgorodskaya E; McGuire KA; Roepstorff P; Svensson B; Clarke AJ
Biochemistry; 1998 Mar; 37(11):3743-52. PubMed ID: 9521693
[TBL] [Abstract][Full Text] [Related]
10. Site-directed mutagenesis of the catalytic base glutamic acid 400 in glucoamylase from Aspergillus niger and of tyrosine 48 and glutamine 401, both hydrogen-bonded to the gamma-carboxylate group of glutamic acid 400.
Frandsen TP; Dupont C; Lehmbeck J; Stoffer B; Sierks MR; Honzatko RB; Svensson B
Biochemistry; 1994 Nov; 33(46):13808-16. PubMed ID: 7947792
[TBL] [Abstract][Full Text] [Related]
11. Identification of enzyme-substrate and enzyme-product complexes in the catalytic mechanism of glucoamylase from Aspergillus awamori.
Natarajan SK; Sierks MR
Biochemistry; 1996 Dec; 35(48):15269-79. PubMed ID: 8952477
[TBL] [Abstract][Full Text] [Related]
12. Stabilization of Aspergillus awamori glucoamylase by proline substitution and combining stabilizing mutations.
Allen MJ; Coutinho PM; Ford CF
Protein Eng; 1998 Sep; 11(9):783-8. PubMed ID: 9796827
[TBL] [Abstract][Full Text] [Related]
13. Protein engineering of the relative specificity of glucoamylase from Aspergillus awamori based on sequence similarities between starch-degrading enzymes.
Sierks MR; Svensson B
Protein Eng; 1994 Dec; 7(12):1479-84. PubMed ID: 7716159
[TBL] [Abstract][Full Text] [Related]
14. Functional roles and subsite locations of Leu177, Trp178 and Asn182 of Aspergillus awamori glucoamylase determined by site-directed mutagenesis.
Sierks MR; Ford C; Reilly PJ; Svensson B
Protein Eng; 1993 Jan; 6(1):75-9. PubMed ID: 8433972
[TBL] [Abstract][Full Text] [Related]
15. Cassette mutagenesis of Aspergillus awamori glucoamylase near its general acid residue to probe its catalytic and pH properties.
Bakir U; Coutinho PM; Sullivan PA; Ford C; Reilly PJ
Protein Eng; 1993 Nov; 6(8):939-46. PubMed ID: 8309943
[TBL] [Abstract][Full Text] [Related]
16. Overexpression and characterization of Aspergillus awamori wild-type and mutant glucoamylase secreted by the methylotrophic yeast Pichia pastoris: comparison with wild-type recombinant glucoamylase produced using Saccharomyces cerevisiae and Aspergillus niger as hosts.
Fierobe HP; Mirgorodskaya E; Frandsen TP; Roepstorff P; Svensson B
Protein Expr Purif; 1997 Mar; 9(2):159-70. PubMed ID: 9056481
[TBL] [Abstract][Full Text] [Related]
17. Identification and elimination by site-directed mutagenesis of thermolabile aspartyl bonds in Aspergillus awamori glucoamylase.
Chen HM; Ford C; Reilly PJ
Protein Eng; 1995 Jun; 8(6):575-82. PubMed ID: 8532682
[TBL] [Abstract][Full Text] [Related]
18. Cloning, heterologous expression, and enzymatic characterization of a thermostable glucoamylase from Talaromyces emersonii.
Nielsen BR; Lehmbeck J; Frandsen TP
Protein Expr Purif; 2002 Oct; 26(1):1-8. PubMed ID: 12356463
[TBL] [Abstract][Full Text] [Related]
19. Structure and energetics of the glucoamylase-isomaltose transition-state complex probed by using modeling and deoxygenated substrates coupled with site-directed mutagenesis.
Frandsen TP; Stoffer BB; Palcic MM; Hof S; Svensson B
J Mol Biol; 1996 Oct; 263(1):79-89. PubMed ID: 8890914
[TBL] [Abstract][Full Text] [Related]
20. Mutational modulation of substrate bond-type specificity and thermostability of glucoamylase from Aspergillus awamori by replacement with short homologue active site sequences and thiol/disulfide engineering.
Fierobe HP; Stoffer BB; Frandsen TP; Svensson B
Biochemistry; 1996 Jul; 35(26):8696-704. PubMed ID: 8679632
[TBL] [Abstract][Full Text] [Related]
[Next] [New Search]