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Title: Pseudomonas aeruginosa exoenzyme S is a biglutamic acid ADP-ribosyltransferase. Author: Radke J, Pederson KJ, Barbieri JT. Journal: Infect Immun; 1999 Mar; 67(3):1508-10. PubMed ID: 10024602. Abstract: Kinetic analysis of two mutations within Pseudomonas aeruginosa exoenzyme S (ExoS) showed that a E379D mutation inhibited expression of ADP-ribosyltransferase activity but had little effect on the expression of NAD glycohydrolase activity while a E381D mutation inhibited expression of both activities. These data identify ExoS as a biglutamic acid ADP-ribosyltransferase, where E381 is the catalytic residue and E379 contributes to the transfer of ADP-ribose to the target protein.[Abstract] [Full Text] [Related] [New Search]