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  • Title: Alginate beads as an affinity material for alpha amylases.
    Author: Sardar M, Gupta MN.
    Journal: Bioseparation; 1998; 7(3):159-65. PubMed ID: 10036753.
    Abstract:
    Calcium-alginate beads were found to bind a variety of enzymes in a nonspecific fashion. However, alpha amylases from porcine pancreas, Bacillus subtilis (BAN 240L) and wheat germ bound at a significant level and B. subtilis and wheat germ amylases could be eluted with 1M maltose. The wheat germ alpha amylase could be purified 45 fold with 70% recovery. The SDS-PAGE pattern showed significant purification by this single step strategy.
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