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  • Title: Cell surface distribution of lectin receptors determined by resonance energy transfer.
    Author: Fernandez SM, Berlin RD.
    Journal: Nature; 1976 Dec 02; 264(5585):411-5. PubMed ID: 1004566.
    Abstract:
    The surface topography of concanavalin A (con A) bound to normal and transformed murine fibroblasts has been studied by a new technique involving fluorescence resonance energy transfer (RET), RET can provide a high resolution "map" of the distances separating con A-receptor complexes in single living cells. The distribution of con A is non-random in both normal and transformed cells, but sites are more closely approximated in the transformed. Approximation is induced by the con A but occurs at extremely slow rates indicating that the topography is not primarily determined by simple diffusion of complexes.
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