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  • Title: Molecular cloning and functional characterization of rat delta-6 fatty acid desaturase.
    Author: Aki T, Shimada Y, Inagaki K, Higashimoto H, Kawamoto S, Shigeta S, Ono K, Suzuki O.
    Journal: Biochem Biophys Res Commun; 1999 Feb 24; 255(3):575-9. PubMed ID: 10049752.
    Abstract:
    Mammalian cDNA fragments putatively encoding amino acid sequences characteristic of the fatty acid desaturase were obtained using expressed sequence tag (EST) sequence informations. These fragments were subsequently used to screen a rat liver cDNA library, yielding a 1573-bp clone. Expression of DNA fragment containing either of two possible open reading frames (nucleotide numbers 97-1431 and 148-1431) of the isolated clone in yeast led to the accumulation of gamma-linolenic acid in the presence of exogenous linoleic acid. In this system, the addition of alpha-linolenic acid also resulted in the accumulation of its Delta-6 desaturated product whereas dihomo-gamma-linolenic acid failed to be a substrate. These results indicate that the protein encoded by the rat cDNA is Delta-6 fatty acid desaturase, and the first 17 amino acids corresponding to the coding region 97-147 of the clone are not required to function in yeast.
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