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  • Title: [Quantitative Coombs' test in autoimmune hemolytic anemia].
    Author: Bally E, Rudolf H, Boreux G, Miescher PA.
    Journal: Schweiz Med Wochenschr; 1976 Oct 02; 106(40):1355-7. PubMed ID: 1006252.
    Abstract:
    The detection of iso- and autoantibodies to red cells and complement on the surface of erythrocytes has been studied in a continuous flow system using a single channel autoanalyzer. A macromolecule, polyvinyl pyrrolidone, is added to the washed cells in order to increase their aggregability. With this system it is possible to establish a direct relationship between the quantity of antibodies present on the surface of the cells and the red cells agglutinated by an antiglobulin serum. By this method it is possible to evaluate the degree of autoantibody coating of erythrocytes in autoimmune hemolytic anemia, and to adjust therapy accordingly.
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