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  • Title: Preparation of a genomic library using a TA vector.
    Author: Kawata Y, Yano S, Thankappan AK, Daniel EC, Kojima H.
    Journal: Prep Biochem Biotechnol; 1999 Feb; 29(1):91-100. PubMed ID: 10069436.
    Abstract:
    An efficient and simple method for constructing a genomic DNA library is presented using a TA cloning vector. It is based on sonication cleavage of genomic DNA, blunting of the fragment ends with mung bean nuclease, and addition of a single 3'-deoxyadenylate with Taq DNA polymerase, followed by ligation with a TA vector. This method is useful for improving the quality of genomic libraries for organisms whose genomic DNA is not well digested with restriction enzymes owing to the presence of polysaccharides and/or DNA methylation.
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