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  • Title: [A simple biochemical method for the determination of "dietary fibre" in plant foods (author's transl)].
    Author: Elchazly M, Thomas B.
    Journal: Z Lebensm Unters Forsch; 1976 Dec 31; 162(4):329-40. PubMed ID: 1007622.
    Abstract:
    A simple enzymatic method for the determination of total waterinsoluble unavailable unavailable carbohydrates (dietary fibre) and their components of hemicellulose, cellulose and lignin is proposed, which would replace the chemical determination of "crude fibre". In this method the sample is treated with amylolytic and proteolytic enzymes. The insoluble residue that remains after the starch and protein have been removed is considered as "unavailable carbohydrates" (dietary fibre) and fractionated to three main components (crude hemicellulose, curde cellulose and crude lignin) by applying separate treatments with 5% and 72% H2SO4. The complete separation process is carried out in a single new quartz-centrifugal tube fitted with a G3 quartzfilter. Experiments on a range of samples indicate that the method is simple, fast and reproducible.
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