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  • Title: Molecular characterization and crystallization of Diocleinae lectins.
    Author: Calvete JJ, Thole HH, Raida M, Urbanke C, Romero A, Grangeiro TB, Ramos MV, Almeida da Rocha IM, Guimarães FN, Cavada BS.
    Journal: Biochim Biophys Acta; 1999 Mar 19; 1430(2):367-75. PubMed ID: 10082964.
    Abstract:
    Molecular characterization of seven Diocleinae lectins was assessed by sequence analysis, determination of molecular masses by mass spectrometry, and analytical ultracentrifugation equilibrium sedimentation. The lectins show distinct pH-dependent dimer-tetramer equilibria, which we hypothesize are due to small primary structure differences at key positions. Lectins from Dioclea guianensis, Dioclea virgata, and Cratylia floribunda seeds have been crystallized and preliminary X-ray diffraction analyses are reported.
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