These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: In vivo distribution and identification of 11C-activity after injection of [methyl-11C]thymidine in Wistar rats.
    Author: Goethals P, van Eijkeren M, Lemahieu I.
    Journal: J Nucl Med; 1999 Mar; 40(3):491-6. PubMed ID: 10086716.
    Abstract:
    UNLABELLED: [Methyl-11C]thymidine and PET offer an in vivo, noninvasive quantitative approach for studying nucleoside uptake in cells on the condition the fraction of [methyl-11C]thymidine (in deoxyribonucleic acid [DNA] or as DNA precursors) versus the total accumulated activity is known. METHODS: In a group of normal (n = 6) and a group of tumor-bearing (n = 3) Wistar rats, the biodistribution of 11C-activity was studied dynamically. In a second group of rats (n = 6), the animals were killed at 20 min postinjection and the organs and tissues of interest (liver, heart, brain, duodenum and tumor) were measured for activity and then homogenized. 11C-activity in each fraction (cell debris, protein/ DNA-fraction, lipids and supernatant) was measured. The supernatant was analyzed by high-performance liquid chromatography (HPLC)-radiochromatography for identification of different 11C-labeled compounds. RESULTS: After venous injection, most of the 11C-activity was rapidly trapped in the liver and in fast-dividing tissue (e.g., duodenum); minor activity was located in the bladder, kidneys, heart and brain. HPLC separation showed that the 11C-activity of the liver tissue consisted of metabolites only. For the duodenum and tumor, at least 55% of the 11C-activity was precipitated in the protein/DNA-fraction and about 60% as DNA precursors (thymidine, 2'-deoxythymidine 5'-monophosphate and 2'-deoxythymidine 5'- triphosphate ) in the supernatant. CONCLUSION: Despite the in vivo metabolism, major 11C-activity in rapidly dividing tissue consists of [methyl-11C]thymidine incorporated in the DNA. Catabolism takes place mainly in the liver where the degradation products are stored. PET quantification data using [methyl-11C] thymidine can give information about thymidine incorporation in DNA and cell proliferation of tumors.
    [Abstract] [Full Text] [Related] [New Search]