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  • Title: The synthesis of lipids from [1-14C]acetate by human venous endothelium in tissue culture.
    Author: Slater DN.
    Journal: Atherosclerosis; 1976; 25(2-3):237-44. PubMed ID: 1008911.
    Abstract:
    Endothelial cells from human umbilical cords were harvested, using a trypsin technique, grown in tissue culture and lipid synthesis studied using [1-14C]acetate as a precursor. Radiosubstrate was incorporated into fatty acids, mono-, di- and triglycerides, cholesterol esters and phospholipids. Radioactivity was also present in the culture medium in the mono-and diglyceride fractions and in the phospholipids running with the solvent front.
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