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  • Title: Cloning, expression and crystallization of pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus.
    Author: Zhou M, Pugmire MJ, Vuong BQ, Ealick SE.
    Journal: Acta Crystallogr D Biol Crystallogr; 1999 Jan; 55(Pt 1):287-90. PubMed ID: 10089427.
    Abstract:
    Pyrimidine nucleoside phosphorylase (PYNP) from B. stearothermophilus has been cloned and purified for crystallization. Crystals of a potential protein-inhibitor complex have been prepared by co-crystallization techniques using the substrate analog pseudouridine. These crystals provide good-quality diffraction images to 2.7 A and belong to space group P21. The asymmetric unit contains the dimer structure of PYNP with unit-cell parameters a = 53.9, b = 71.9, c = 123.3 A and beta = 96.9 degrees.
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