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Title: Isolation of an active catalytic core of Streptococcus downei MFe28 GTF-I glucosyltransferase. Author: Monchois V, Arguello-Morales M, Russell RR. Journal: J Bacteriol; 1999 Apr; 181(7):2290-2. PubMed ID: 10094712. Abstract: Truncated variants of GTF-I from Streptococcus downei MFe28 were purified by means of a histidine tag. Sequential deletions showed that the C-terminal domain was not directly involved in the catalytic process but was required for primer activation. A fully active catalytic core of only 100 kDa was isolated.[Abstract] [Full Text] [Related] [New Search]