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  • Title: Resolution and purification of histones on homologous series of hydrocarbon-coated agaroses.
    Author: Arfmann HA, Shaltiel S.
    Journal: Eur J Biochem; 1976 Nov 01; 70(1):269-74. PubMed ID: 1009930.
    Abstract:
    Hydrophobic chromatography on alkyl-agarose columns has been applied to the fractionation of histones. This paper describes: (a) a two-column method for the resolution of whole histone from calf thymus into its five main components (H1, H2a, H2b, H3 and H4), (b) a rapid one-step procedure for the isolation of the H3 fraction from whole histone, (c) an alternative one-step procedure for the resolution of H3 and H2a (which co-elute during gel exclusion chromatography on Biogel P-60). These experiments are also used for gaining further insight into the mechanism of action of hydrocarbon-coated agaroses.
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