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  • Title: Influence of glycerol concentration and freezing rate on post-thaw motility of bovine spermatozoa in Continental straws.
    Author: Mortimer RG, Berndtson WE, Ennen BD, Pickett BW.
    Journal: J Dairy Sci; 1976 Dec; 59(12):2134-7. PubMed ID: 1010884.
    Abstract:
    Semen from each of six bulls was used to study the effects of percent glycerol and rate of freezing on spermatozoa extended in egg yolk-sodium citrate extender and packaged in .25-ml Continental straws. Treatments were arranged factorially and included 5, 6, 7, 8, 9, 10, or 11% (volume/volume) glycerol; freezing rates (time from 5 to -130 C) of 1, 2, 3.5, 7, 12, or 20 min; and post-thaw incubation times of 0 and 2 h at 38 C. Post-thaw motility was similar for spermatozoa frozen in 2 to 20 min, and all means were greater than for spermatozoa frozen in 1 min. Post-thaw motility of spermatozoa frozen in 7% glycerol did not differ from that with 5 to 9% but was superior to that with 10 or 11% glycerol. Although glycerol and freezing rate interacted, 7% glycerol provided survival equal to or greater than that with the other percents of glycerol, regardless of freezing rate.
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